##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926883_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 580343 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.832009690820772 31.0 28.0 31.0 16.0 34.0 2 28.225406354517933 31.0 28.0 31.0 16.0 34.0 3 27.82680070234327 31.0 28.0 31.0 16.0 34.0 4 31.670946319676467 35.0 32.0 37.0 19.0 37.0 5 31.84956482631823 35.0 32.0 37.0 19.0 37.0 6 30.861290650529085 35.0 31.0 37.0 11.0 37.0 7 30.666316299154122 35.0 30.0 37.0 11.0 37.0 8 30.770039442191944 35.0 32.0 37.0 11.0 37.0 9 33.010188802139425 37.0 33.0 39.0 17.0 39.0 10 33.164144307762825 37.0 33.0 39.0 17.0 39.0 11 33.32765623088415 37.0 33.0 39.0 18.0 39.0 12 33.19517595628792 38.0 33.0 39.0 17.0 39.0 13 33.31697978609202 38.0 34.0 39.0 17.0 39.0 14 34.334817513091394 39.0 34.0 40.0 17.0 41.0 15 33.896817916301224 39.0 33.0 40.0 11.0 41.0 16 33.990254039421515 39.0 33.0 40.0 17.0 41.0 17 34.209674278831656 39.0 34.0 41.0 17.0 41.0 18 34.25299176521471 39.0 34.0 41.0 17.0 41.0 19 33.64085894031633 39.0 33.0 40.0 10.0 41.0 20 33.91272402699783 39.0 34.0 41.0 10.0 41.0 21 33.91035646160977 39.0 34.0 41.0 10.0 41.0 22 33.9261195534365 39.0 34.0 41.0 8.0 41.0 23 33.90291948037626 39.0 34.0 41.0 2.0 41.0 24 32.61250501858384 38.0 32.0 40.0 2.0 41.0 25 33.184223812469526 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.26738457233085455 1101 2 -0.3267619616451043 1101 3 -1.1357207154212716 1101 4 -0.3104903616761234 1101 5 -0.13249066492264205 1101 6 -2.20730101861038 1101 7 -0.7472764182021336 1101 8 -0.326051265635229 1101 9 -0.393240937629038 1101 10 -9.278405512144502 1101 11 -4.102183470174701 1101 12 -1.6767385318999715 1101 13 -0.8921948287384893 1101 14 -0.855387222345648 1101 15 -2.0958606015983463 1101 16 -0.5017617387846656 1101 17 -0.3979883794487975 1101 18 -0.18782035864879987 1101 19 -0.7931666407505915 1101 20 -0.20699594442028513 1101 21 -0.2360611639265784 1101 22 -0.11359068855741583 1101 23 0.019298818354833713 1101 24 -0.35170803946075324 1101 25 -0.12000189505872072 1102 1 -0.2478350656664965 1102 2 -0.05998205858123029 1102 3 -0.558096083259251 1102 4 -0.2164644831924818 1102 5 0.18544035463768438 1102 6 -0.904884106718395 1102 7 -0.1769760893117045 1102 8 0.28147643040077597 1102 9 0.36004796664653327 1102 10 0.44680869938397194 1102 11 0.26389104947217845 1102 12 0.4004425309354076 1102 13 0.02122260245417351 1102 14 -0.23979954708167384 1102 15 0.553611442715308 1102 16 0.3017803245700179 1102 17 0.02096825894483345 1102 18 -0.13934147687625398 1102 19 0.20600031441644973 1102 20 0.3746532679736916 1102 21 0.007512490219170331 1102 22 0.11510716499979168 1102 23 -0.2879216247817382 1102 24 -3.1030928412609917 1102 25 -1.635883840705251 1103 1 -0.22252160232011775 1103 2 -0.2530817656146134 1103 3 0.054238303150597744 1103 4 -0.2665735258038211 1103 5 -0.2937281339606663 1103 6 0.17557103003579755 1103 7 -0.21919935610809205 1103 8 0.0062996119307605625 1103 9 -0.004862175682767145 1103 10 0.050091729183485256 1103 11 0.07123680473573302 1103 12 -0.300963818132125 1103 13 -0.029400224892292215 1103 14 -0.13956233157630038 1103 15 0.5178788075461966 1103 16 0.1759918836333938 1103 17 -0.18378998893989973 1103 18 -0.02438897463527212 1103 19 -1.4081897094144153 1103 20 -0.45486448492864895 1103 21 -0.20612904107797192 1103 22 -0.1714111782084089 1103 23 -0.11432934029804187 1103 24 1.1818199246499361 1103 25 0.5750439233473088 1104 1 0.011961430290376995 1104 2 -0.001651869901984071 1104 3 -0.9859815280031867 1104 4 -0.08188092524099844 1104 5 0.15899426299348107 1104 6 -1.3270795245081501 1104 7 -0.27822137233057376 1104 8 0.30563680775926727 1104 9 0.8550749208244355 1104 10 0.7507399662303342 1104 11 0.6628977880166502 1104 12 0.576482962202256 1104 13 0.40015117388274746 1104 14 0.36261015642237027 1104 15 0.805603356461944 1104 16 0.044180594111793425 1104 17 0.21197230207152273 1104 18 0.4441639139593292 1104 19 -0.9433514376051093 1104 20 -0.02303002043601765 1104 21 0.08628877054262318 1104 22 0.11684705718307953 1104 23 0.1866592377519538 1104 24 1.6614597193859098 1104 25 1.0766363749281709 1105 1 -0.6049682853029559 1105 2 -0.4751438607486662 1105 3 0.04126005421322532 1105 4 -0.3947517980752586 1105 5 -0.19347517146222515 1105 6 -0.5736672982904345 1105 7 -0.47921908400105195 1105 8 -0.039836876451261105 1105 9 -0.8384916041837371 1105 10 -0.32950978859150126 1105 11 -0.4939103192079344 1105 12 -0.5874411960193839 1105 13 -0.6619867796968535 1105 14 -0.8551982777064282 1105 15 -0.21540220090975026 1105 16 -0.8711747507721554 1105 17 -0.7578544386934354 1105 18 -0.840004768616673 1105 19 -1.7503838011225987 1105 20 -1.1101460714330713 1105 21 -0.9789626188003027 1105 22 -0.8613915828953793 1105 23 -0.8751021221695865 1105 24 -5.413857704608862 1105 25 -3.02956826711171 1106 1 -0.1251814268524818 1106 2 -0.6690804413305536 1106 3 -0.3448386708603266 1106 4 -0.5630237823838549 1106 5 -0.023862879863663977 1106 6 -0.04822238165100856 1106 7 -2.6907928009020026 1106 8 -0.4029346208121609 1106 9 -0.5117822220327071 1106 10 0.07188282337319407 1106 11 -0.3245307834119231 1106 12 0.12048296220226007 1106 13 -0.12784882611725834 1106 14 -0.6419612721490537 1106 15 0.25017478503337287 1106 16 0.09103773696893569 1106 17 -0.29202769792848216 1106 18 -0.5661218003263855 1106 19 0.04407713382346401 1106 20 0.10839855099255402 1106 21 -0.08513980088594764 1106 22 -0.33458151424549243 1106 23 -0.2784836193909044 1106 24 -3.2356831377569506 1106 25 -1.6285064822146822 1107 1 0.22078230206893323 1107 2 0.2379205131715132 1107 3 0.4498644789964956 1107 4 0.43296349092189956 1107 5 0.19723543384935027 1107 6 0.2696993491794082 1107 7 -2.1975354035109724 1107 8 -1.1022562880091087 1107 9 -0.1125483690260296 1107 10 0.209738375393556 1107 11 -0.06075859123883731 1107 12 0.281609274642598 1107 13 0.06061569822254853 1107 14 0.2026826985795438 1107 15 -1.0504615496786869 1107 16 -0.35061546061299254 1107 17 -0.20288611345504393 1107 18 -0.37392199122680125 1107 19 0.6067204682173539 1107 20 0.6097170365159386 1107 21 0.30642621884042853 1107 22 0.012785969050725043 1107 23 -0.31164047589742694 1107 24 -4.474363379564139 1107 25 -2.3488361035955307 1108 1 0.4983381280485162 1108 2 0.20033667534429256 1108 3 0.90590654268901 1108 4 0.786505918156152 1108 5 0.8071997940031359 1108 6 -2.708563406033271 1108 7 -1.0105741787452338 1108 8 0.581824665720319 1108 9 0.21205741141517365 1108 10 0.6690944090383866 1108 11 0.3351766294501317 1108 12 0.6789578435637367 1108 13 0.38455307209907374 1108 14 0.41446680836149596 1108 15 -0.6614272440944333 1108 16 0.5710521372307582 1108 17 0.5438656092225571 1108 18 0.6323347534291344 1108 19 0.6308838757642263 1108 20 -0.8034181729478789 1108 21 0.12745780997970257 1108 22 0.5533176839217475 1108 23 0.5990774999021724 1108 24 -0.5065298076964027 1108 25 0.20877186830077932 1109 1 0.9673824163839271 1109 2 0.8369852982396111 1109 3 -0.2608323497478118 1109 4 0.9579698965036272 1109 5 1.2515720126774248 1109 6 1.0822276815601164 1109 7 1.4590441143950912 1109 8 1.8580438874052874 1109 9 1.0808903443389681 1109 10 1.285043379631091 1109 11 1.0660596084970564 1109 12 0.8030556549961858 1109 13 0.9908022484718728 1109 14 0.9871373372061925 1109 15 -0.4749200316543707 1109 16 0.9408582173734885 1109 17 0.578375589050026 1109 18 1.0299376181312638 1109 19 -0.5295878472132003 1109 20 0.7911773120545007 1109 21 1.098303941212663 1109 22 1.2202060963739783 1109 23 1.2402028534283147 1109 24 0.16919802532775208 1109 25 0.7048032522985892 1110 1 0.4076479031095914 1110 2 0.5571180416024397 1110 3 1.0289906591017441 1110 4 0.7975716664151307 1110 5 0.7936074109075157 1110 6 0.964971044391973 1110 7 0.9800618135101331 1110 8 1.259592559971658 1110 9 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21 1.00239105196966 1111 22 1.20459962187455 1111 23 0.7545122464795568 1111 24 1.1309032961735355 1111 25 0.8705546110329081 1112 1 0.5490733994023458 1112 2 0.7200778598277466 1112 3 -1.395124385146044 1112 4 0.16185652649645377 1112 5 0.3578205178197358 1112 6 1.3452293558007256 1112 7 1.5029678168586145 1112 8 1.7328800510025104 1112 9 0.6678934536429679 1112 10 0.8457824372728027 1112 11 0.7272375563564211 1112 12 0.9629540046732998 1112 13 0.41683071056228016 1112 14 0.8309653687775835 1112 15 1.3514875263461121 1112 16 1.2344817524129539 1112 17 0.5313545414537586 1112 18 0.9816465394419538 1112 19 1.4861775199238494 1112 20 1.2958580104520152 1112 21 1.1495165697704195 1112 22 1.1849860533220777 1112 23 1.096898619991336 1112 24 2.2272666691928578 1112 25 1.4216556799474773 1113 1 -0.23519704467517144 1113 2 0.17761501112695655 1113 3 -4.350322670247866 1113 4 -0.9992975536286899 1113 5 0.2547933749212099 1113 6 1.0502274728888779 1113 7 1.417358477615835 1113 8 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0.5142588302319488 2215 2 -0.09091976356251408 2215 3 0.3316649809071279 2215 4 0.49200630817997393 2215 5 0.4565437809601782 2215 6 1.1630273992429423 2215 7 0.9336559282829242 2215 8 -0.37817505318142963 2215 9 -0.043446843709602945 2215 10 -0.2152687980115573 2215 11 -0.38088602734998744 2215 12 -0.6967475373595278 2215 13 -0.25549007648243105 2215 14 -0.44459755611049445 2215 15 -0.6289955334007544 2215 16 -0.1325894906092131 2215 17 -0.8138529973734094 2215 18 -1.4562111955936956 2215 19 -0.07968331023145936 2215 20 -4.036975682136276 2215 21 -2.4013793568310255 2215 22 -1.5099434774356766 2215 23 -1.36458703744524 2215 24 -1.5213366580607754 2215 25 -1.2518357255684656 2216 1 -0.7103860600571146 2216 2 -1.5293192295693423 2216 3 -0.5570786471003046 2216 4 -0.9269168622769364 2216 5 -1.0945703345711166 2216 6 -1.8540637834924105 2216 7 -10.64052490663411 2216 8 -11.380300822178363 2216 9 -6.329528583779073 2216 10 -3.9228246313342616 2216 11 -2.9957562066373455 2216 12 -2.733844332125038 2216 13 -2.1426287488971774 2216 14 -2.386124028311812 2216 15 -3.526807691949106 2216 16 -2.5766201186889184 2216 17 -2.9744250843258655 2216 18 -2.5780624002669867 2216 19 -2.086670712924384 2216 20 -2.949090014496008 2216 21 -5.6542672140133625 2216 22 -4.175970287634264 2216 23 -4.034535891443177 2216 24 -3.8676605657343757 2216 25 -3.410277791985994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34676.0 3 6.0 4 268.0 5 1619.0 6 1890.0 7 1593.0 8 1228.0 9 1169.0 10 1037.0 11 1098.0 12 1117.0 13 1036.0 14 1093.0 15 1099.0 16 1182.0 17 1319.0 18 1566.0 19 1857.0 20 2366.0 21 3176.0 22 3980.0 23 4650.0 24 5455.0 25 6130.0 26 7120.0 27 7893.0 28 8648.0 29 10116.0 30 12280.0 31 15050.0 32 19269.0 33 25115.0 34 33725.0 35 47614.0 36 69420.0 37 107974.0 38 135509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4011436624486 18.364227647892896 12.747639533899696 25.48698915575881 2 14.182686501240523 19.995031004461744 42.04425856720394 23.778023927093795 3 19.18402418319599 24.099686667494858 29.060727152263034 27.655561997046117 4 11.753813049370496 16.911559324254892 38.319637505991565 33.01499012038305 5 12.72820967110921 37.76513512907968 36.56990501927707 12.936750180534037 6 31.65010728411764 37.0115556627918 18.15454083259369 13.183796220496868 7 27.619210568612505 31.133094488774134 22.069963136364343 19.177731806249017 8 24.460889191515246 37.526743836388206 21.283773274482492 16.728593697614052 9 25.896174712707072 15.70684057051861 20.128945753855273 38.26803896291905 10 14.347176328839662 29.53775667974951 34.81347033262145 21.30159665878938 11 34.43399315476909 22.758841756597928 24.058263737854542 18.748901350778446 12 22.936970380059662 24.964713546379066 31.926011329579783 20.172304743981485 13 28.935772006045557 22.04576905909582 25.556085968210855 23.462372966647767 14 20.364772547100227 22.445592604592488 27.58404968875592 29.605585159551367 15 23.136060717295432 31.136570830812925 23.06092237485416 22.66644607703748 16 21.736118172401966 28.40253206879258 28.21347139186229 21.647878366943164 17 21.49710206621042 28.774940068006227 27.899441783672646 21.82851608211071 18 21.358689564250817 28.195221148957806 29.567525182459736 20.878564104331645 19 23.664190551696798 27.096913186604848 27.0777821823133 22.16111407938505 20 23.155656125753342 27.87123414683747 28.074595818677217 20.89851390873197 21 22.78495846689415 27.11784372522662 27.03908592699824 23.058111880880986 22 22.546549382673494 28.383748905632807 27.3076498852207 21.762051826473 23 22.216975360238244 27.884103329478616 27.528216723740652 22.370704586542487 24 23.171019637917812 27.20441874693023 27.968737882482703 21.65582373266926 25 22.59799570878321 27.40381384046669 27.931168193294326 22.067022257455772 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 148.0 1 148.0 2 210.0 3 272.0 4 272.0 5 272.0 6 400.5 7 529.0 8 529.0 9 529.0 10 808.0 11 1087.0 12 1087.0 13 1087.0 14 1819.0 15 2551.0 16 2551.0 17 2551.0 18 4610.0 19 6669.0 20 6669.0 21 6669.0 22 10376.0 23 14083.0 24 14083.0 25 14083.0 26 20454.0 27 26825.0 28 26825.0 29 26825.0 30 35483.0 31 44141.0 32 44141.0 33 44141.0 34 50178.5 35 56216.0 36 56216.0 37 56216.0 38 60902.0 39 65588.0 40 65588.0 41 65588.0 42 69055.5 43 72523.0 44 72523.0 45 72523.0 46 74708.0 47 76893.0 48 76893.0 49 76893.0 50 74084.0 51 71275.0 52 71275.0 53 71275.0 54 62987.5 55 54700.0 56 54700.0 57 54700.0 58 46981.5 59 39263.0 60 39263.0 61 39263.0 62 31895.0 63 24527.0 64 24527.0 65 24527.0 66 18920.0 67 13313.0 68 13313.0 69 13313.0 70 9697.0 71 6081.0 72 6081.0 73 6081.0 74 4257.0 75 2433.0 76 2433.0 77 2433.0 78 1640.0 79 847.0 80 847.0 81 847.0 82 555.5 83 264.0 84 264.0 85 264.0 86 176.5 87 89.0 88 89.0 89 89.0 90 53.0 91 17.0 92 17.0 93 17.0 94 11.0 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22848556801753445 2 0.12906160667053793 3 0.13216322071602482 4 0.062204592801153806 5 0.02067742696991262 6 0.018609684272921357 7 0.29706570080107797 8 0.2937917748641751 9 0.017058877250177913 10 0.03342850693469207 11 0.014646510770354774 12 0.01585269401026634 13 0.014129575096106958 14 0.015680382118850405 15 0.013784951313275081 16 0.01826506049008948 17 0.01757581292442573 18 0.013440327530443203 19 0.02291748155831982 20 0.016541941575930096 21 0.014301886987522897 22 0.017058877250177913 23 0.01757581292442573 24 0.015163446444602588 25 0.015163446444602588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 580343.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.07561457991682 #Duplication Level Percentage of deduplicated Percentage of total 1 94.20162767428313 82.02664624166704 2 4.3997581078259245 7.662232824838286 3 0.6985958372195759 1.824919856065983 4 0.24742338827964724 0.8617817438358268 5 0.11940859878838896 0.5198788562812839 6 0.07102549462039463 0.3710753156948064 7 0.045206160997355034 0.27554479761503486 8 0.035616658274123254 0.24810739268017296 9 0.02445959662548791 0.19168509676870973 >10 0.13090154114917543 2.169007142096704 >50 0.012691060000163802 0.7737290309889483 >100 0.011501211228809888 1.9729058211946693 >500 0.001586373962516663 0.9217023694432576 >1k 1.9829674531458288E-4 0.1807835108293538 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1047 0.18041055031248762 No Hit GTCCTACAGTGTGCATTTCTCATTT 769 0.13250784449885672 No Hit GTCCTAAAGTGTGTATTTCTCATTT 762 0.13130166125894513 No Hit GTATCAACGCAGAGTACTTTTTTTT 749 0.12906160667053793 No Hit CTGTAGGACGTGGAATATGGCAAGA 737 0.12699386397354667 No Hit GTACATGGGAAGCAGTGGTATCAAC 689 0.11872289318558163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAC 35 0.0021132415 16.348175 1 ATCTATG 40 0.005141529 14.304653 1 GTACTAG 40 0.005141529 14.304653 1 ACCGTCT 40 0.005239885 14.263867 8 GTCTTAG 100 9.496944E-9 13.351009 1 GGTGAGC 60 4.0517526E-4 12.678993 8 TAGACAA 70 1.070972E-4 12.235686 5 ACTGATC 70 1.0787806E-4 12.226172 8 AGTACCT 55 0.003024526 12.112093 5 GTCTTAA 95 9.891919E-7 12.046023 1 GTCCTAC 610 0.0 11.88146 1 TATAGGA 105 2.5973895E-7 11.799858 2 TGAGTTA 190 0.0 11.480442 12 ATAGGAC 75 2.0085038E-4 11.436786 3 TGTAGGA 650 0.0 11.436785 2 TAGGACC 225 0.0 11.421949 4 TAGTACT 75 2.032878E-4 11.421949 4 GTATTAA 85 5.1061434E-5 11.219336 1 GTATAAG 85 5.1061434E-5 11.219336 1 CTGTAGG 700 0.0 11.171252 1 >>END_MODULE