FastQCFastQC Report
Thu 26 May 2016
SRR1926883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580343
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12740.21952534966390566No Hit
GTCCTACAGTGGACATTTCTAAATT10560.18196135733523106No Hit
CTGTAGGACGTGGAATATGGCAAGA9570.16490248008505315No Hit
GGTATCAACGCAGAGTACTTTTTTT8540.14715435526921147No Hit
GTCCTACAGTGTGCATTTCTCATTT7820.13474789908726392No Hit
GTACATGGGAAGCAGTGGTATCAAC7790.1342309634130161No Hit
GTCCTAAAGTGTGTATTTCTCATTT7200.12406456181947571No Hit
CCCATGTACTCTGCGTTGATACCAC7180.12371993803664384No Hit
CTTTAGGACGTGAAATATGGCGAGG7050.12147988344823665No Hit
TATCAACGCAGAGTACTTTTTTTTT6730.1159659029229266No Hit
CTGTAGGACCTGGAATATGGCGAGA6130.1056271894379703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG456.599059E-414.822861
TCTAGGA1350.014.1157882
CTAGGAC1204.9112714E-1113.4842253
TATTAGG500.0014645313.339422
GGCGAGG2050.012.96248719
TAGGACA2000.012.8474544
ATAGGAC1051.9310392E-812.6910363
CTTAGAC701.0692618E-412.2377843
TCCAACG701.0989609E-412.20193318
AATCCCG1251.4242687E-912.14770219
GTCTAAG550.002995315212.1277951
TAGACCT550.003024561312.1120774
CAACCCT550.003024561312.1120774
TAGGACC2950.011.9361154
TAGAGTG907.2944113E-611.6314395
GCATAAG752.0117885E-411.4347781
CCAGACT752.0361788E-411.4199594
TAGGACG5700.011.3531754
AGGACGT5800.011.3215115
TGGCGAG5250.011.20770318
Produced by FastQC (version 0.11.5)