FastQCFastQC Report
Thu 26 May 2016
SRR1926882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542380
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15030.27711198790515873No Hit
GTCCTAAAGTGTGTATTTCTCATTT13380.24669051218702753No Hit
CTGTAGGACGTGGAATATGGCAAGA13270.24466241380581877No Hit
GTCCTACAGTGTGCATTTCTCATTT10660.19654117039713853No Hit
CTTTAGGACGTGAAATATGGCGAGG10580.19506618975625944No Hit
GTACATGGGAAGCAGTGGTATCAAC8720.16077288985582064No Hit
GTATCAACGCAGAGTACTTTTTTTT7730.14252000442494192No Hit
CCCATGTACTCTGCGTTGATACCAC7260.13385449315977727No Hit
CTGTAGGACCTGGAATATGGCGAGA6520.12021092223164571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG307.569144E-419.0535584
GTTCTAG400.005118604714.3140431
CCTGTAC903.443529E-813.7749433
GTCCTAT500.001447574913.3597731
CCAACGA500.001521052613.2723119
GTGTAGG956.933078E-813.0584251
GATATAC1550.012.9288121
TTCTAGA603.9543078E-412.7153332
TAGGACC2450.012.4431414
ACTGTTC1800.012.1550868
ATAAGAC550.002977224812.1373633
CTGTACT1103.6470738E-812.1249914
AGAGGTC550.003012582212.1182555
GGCGAGG2600.012.0325919
TGTACTG1353.5470293E-1011.9900195
GTACATA1209.309588E-911.9283691
ACTGGGG657.919484E-411.707918
TCCAACG907.6099186E-611.58693718
GTCCTAC9000.011.4512341
CTCTAAA751.9846116E-411.4512341
Produced by FastQC (version 0.11.5)