##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926882_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 542380 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.032656071389063 31.0 28.0 31.0 16.0 34.0 2 28.45679597330285 31.0 28.0 31.0 20.0 34.0 3 28.015743574615584 31.0 28.0 31.0 16.0 34.0 4 31.876765367454553 35.0 32.0 37.0 19.0 37.0 5 32.04502747151444 35.0 33.0 37.0 19.0 37.0 6 31.08014860429957 35.0 32.0 37.0 17.0 37.0 7 30.892626940521406 35.0 31.0 37.0 15.0 37.0 8 30.998818171761496 35.0 32.0 37.0 13.0 37.0 9 33.202996054426784 37.0 33.0 39.0 17.0 39.0 10 33.348043806925034 37.0 33.0 39.0 17.0 39.0 11 33.52001548729673 38.0 34.0 39.0 19.0 39.0 12 33.40305136620082 38.0 34.0 39.0 18.0 39.0 13 33.51489361702128 38.0 34.0 39.0 19.0 39.0 14 34.53239794977691 39.0 34.0 41.0 19.0 41.0 15 34.11670784320956 39.0 33.0 41.0 17.0 41.0 16 34.20921309782809 39.0 34.0 40.0 17.0 41.0 17 34.432027360890885 39.0 34.0 41.0 18.0 41.0 18 34.46750064530403 39.0 34.0 41.0 18.0 41.0 19 33.84713669383089 39.0 33.0 41.0 10.0 41.0 20 34.127908477451236 39.0 34.0 41.0 16.0 41.0 21 34.11519967550426 39.0 34.0 41.0 11.0 41.0 22 34.140764777462294 39.0 34.0 41.0 10.0 41.0 23 34.13816328035695 39.0 34.0 41.0 8.0 41.0 24 32.84265459640842 39.0 32.0 40.0 2.0 41.0 25 33.427419890113946 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.008646926042018777 1101 2 0.08251209976147678 1101 3 -0.9229528646999121 1101 4 -0.09941931649241553 1101 5 0.10228473467465449 1101 6 -1.9164982091610838 1101 7 -0.40809835495914726 1101 8 0.1592810511312237 1101 9 0.2315924756067389 1101 10 -8.70526444452926 1101 11 -4.033873435290214 1101 12 -1.1591493509679225 1101 13 -0.39259030664737793 1101 14 -0.08503781699635482 1101 15 -1.3858614776409581 1101 16 0.10119940927689441 1101 17 0.29944658036970395 1101 18 0.3560029155328337 1101 19 -0.24789343762446236 1101 20 0.19144498373181307 1101 21 -0.08627342702613561 1101 22 0.29855006111899485 1101 23 0.17118127354901702 1101 24 0.02830947051560173 1101 25 0.015818144616567054 1102 1 -0.2869343454982314 1102 2 -0.23365908276908698 1102 3 -0.6887007920102235 1102 4 -0.3797001998022438 1102 5 -0.2570447817340735 1102 6 -1.187322397130611 1102 7 -0.37632852858874344 1102 8 -0.0330035307972274 1102 9 -0.14059814646480362 1102 10 -0.10511220340921312 1102 11 -0.06691972094935039 1102 12 -0.004886132609321692 1102 13 -0.15339584585802868 1102 14 -0.41235622509595515 1102 15 0.04836195254761577 1102 16 -0.032654470381011436 1102 17 -0.46584991621654126 1102 18 -0.5266250962105943 1102 19 0.03311129150154102 1102 20 -0.1718633496793558 1102 21 -0.3070822040847361 1102 22 -0.2008537389937075 1102 23 -0.6821675640946978 1102 24 -4.007775410913787 1102 25 -2.4374214156587755 1103 1 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-1.3512419898318129 2214 25 -0.5979035255421934 2215 1 0.5629704330368313 2215 2 -0.07102136633806566 2215 3 0.49644356809607615 2215 4 0.684240058960043 2215 5 0.6377368637146716 2215 6 1.5269231705693933 2215 7 0.8645190243014618 2215 8 -0.36148757233452145 2215 9 0.13952757860982246 2215 10 0.022741030027980003 2215 11 0.03959189934346341 2215 12 -0.28796945275534824 2215 13 0.08469328579860758 2215 14 0.12113499215632118 2215 15 0.18518073709608984 2215 16 0.40246669825101833 2215 17 -0.17293886779820156 2215 18 -0.8573955442698775 2215 19 0.43737415731293794 2215 20 -3.581181956881263 2215 21 -1.8915428249614266 2215 22 -1.0022910647166015 2215 23 -0.613930061570926 2215 24 -0.7870389588273738 2215 25 -0.45605099051812203 2216 1 -0.02019799730266314 2216 2 -0.956024581323387 2216 3 -0.07215275435947532 2216 4 -0.26509775375335565 2216 5 -0.4114225048522471 2216 6 -1.2598659812227737 2216 7 -9.223686216373835 2216 8 -10.329719031339721 2216 9 -5.371208977019393 2216 10 -2.9537821004206712 2216 11 -2.0734410031652253 2216 12 -1.888795379992004 2216 13 -1.4743502934520265 2216 14 -1.5397314090040695 2216 15 -2.710424452737577 2216 16 -1.6698854248854076 2216 17 -1.7473869619848088 2216 18 -1.3897946211454126 2216 19 -0.8752669655892191 2216 20 -1.6863942206795315 2216 21 -4.488124473127389 2216 22 -3.1230698388352245 2216 23 -2.633720913068508 2216 24 -2.7397836590860685 2216 25 -2.3091884672780196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30062.0 3 0.0 4 209.0 5 1416.0 6 1827.0 7 1475.0 8 1106.0 9 1095.0 10 983.0 11 918.0 12 919.0 13 943.0 14 970.0 15 1022.0 16 1089.0 17 1211.0 18 1334.0 19 1629.0 20 2160.0 21 2913.0 22 3558.0 23 4256.0 24 4902.0 25 5610.0 26 6461.0 27 7170.0 28 8040.0 29 9587.0 30 11205.0 31 13863.0 32 17959.0 33 23631.0 34 32412.0 35 44679.0 36 65065.0 37 101735.0 38 128966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43247087422579 19.106544825152994 12.705432070031161 25.75555223059005 2 13.559124939088319 20.442329558039603 43.226990150765644 22.771555352106436 3 18.657154459539278 25.256755759007675 29.44569551092735 26.64039427052569 4 11.491824632745013 17.544290158193636 39.09434421783267 31.869540991228686 5 12.139145123871838 38.363386899866114 37.087969844390265 12.40949813187178 6 30.312871126454077 37.86448767030324 18.900248585570235 12.922392617672443 7 26.58900753478482 31.415152775851706 23.22562751352101 18.770212175842463 8 23.4952655261533 38.24238435571594 22.14498840876571 16.117361709365053 9 26.063977900756253 15.555957151366172 20.772256450069246 37.60780849780833 10 14.357962300343061 29.63406986609613 35.344719465860045 20.663248367700763 11 33.28858565369722 22.650009219988938 25.71952793656648 18.341877189747372 12 23.21069975087268 24.904249178949385 32.72658876290124 19.158462307276693 13 28.863122140656206 22.71340086040778 25.921029096387432 22.50244790254858 14 19.78952298761553 23.325072101377117 27.985660861676 28.899744049331353 15 22.342632391257446 32.79360811929642 23.190666644539224 21.673092844906908 16 20.72656862293117 28.527776497164727 29.81826564012724 20.92738923977687 17 20.844758752316586 29.395244198161485 28.814184423319837 20.94581262620209 18 20.688275383911844 28.028699346501647 31.198830193689243 20.084195075897266 19 23.446305803900742 27.186974417987074 27.51818329349975 21.848536484612435 20 23.022709042126518 28.335868853668206 28.629620409555685 20.01180169464959 21 22.011395696188526 27.443528609098117 27.45293282439933 23.092142870314028 22 22.35134447366189 29.452105315566833 27.50242488391906 20.694125326852227 23 21.147133181262458 28.925429335910056 28.46147179281972 21.465965690007764 24 23.06558979181649 27.59491803580978 28.837934022975787 20.501558149397948 25 21.98558020320481 28.13630580295403 28.587154948276815 21.290959045564346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 161.0 1 161.0 2 240.0 3 319.0 4 319.0 5 319.0 6 444.0 7 569.0 8 569.0 9 569.0 10 868.0 11 1167.0 12 1167.0 13 1167.0 14 1974.0 15 2781.0 16 2781.0 17 2781.0 18 4837.5 19 6894.0 20 6894.0 21 6894.0 22 10940.5 23 14987.0 24 14987.0 25 14987.0 26 21846.5 27 28706.0 28 28706.0 29 28706.0 30 38268.5 31 47831.0 32 47831.0 33 47831.0 34 52978.0 35 58125.0 36 58125.0 37 58125.0 38 61675.5 39 65226.0 40 65226.0 41 65226.0 42 67387.0 43 69548.0 44 69548.0 45 69548.0 46 70966.0 47 72384.0 48 72384.0 49 72384.0 50 67449.0 51 62514.0 52 62514.0 53 62514.0 54 53671.0 55 44828.0 56 44828.0 57 44828.0 58 37770.5 59 30713.0 60 30713.0 61 30713.0 62 24655.5 63 18598.0 64 18598.0 65 18598.0 66 14190.0 67 9782.0 68 9782.0 69 9782.0 70 7160.5 71 4539.0 72 4539.0 73 4539.0 74 3159.5 75 1780.0 76 1780.0 77 1780.0 78 1209.5 79 639.0 80 639.0 81 639.0 82 408.5 83 178.0 84 178.0 85 178.0 86 129.5 87 81.0 88 81.0 89 81.0 90 49.0 91 17.0 92 17.0 93 17.0 94 12.5 95 8.0 96 8.0 97 8.0 98 6.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18824440429219366 2 0.11283601902725027 3 0.11431099966812935 4 0.07116781592241603 5 0.023230945093845642 6 0.02064972897230724 7 0.2866993620708728 8 0.2680777314797743 9 0.01825288543087872 10 0.03687451602197721 11 0.014749806408790884 12 0.016040414469560088 13 0.014565433828681 14 0.016224787049669974 15 0.012352962867362365 16 0.019359120911538037 17 0.017515395110439175 18 0.012537335447472252 19 0.02286219993362587 20 0.015671669309340313 21 0.012906080607692024 22 0.015856041889450202 23 0.01640915962977986 24 0.012906080607692024 25 0.012906080607692024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 542380.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.92402963821428 #Duplication Level Percentage of deduplicated Percentage of total 1 93.7561785983937 78.68396311457305 2 4.6601443357524825 7.821961827040955 3 0.7430103058624251 1.870692567920905 4 0.27569660646689037 0.9255028068912963 5 0.14000613744794557 0.5874939614356642 6 0.0864042818556033 0.4350837306790962 7 0.059932418605463514 0.3520839052734349 8 0.04053381218531487 0.2721408684152141 9 0.03146910436403394 0.23769126426017184 >10 0.1656968974231445 2.6730355675413384 >50 0.02112388336945822 1.2477933474221101 >100 0.01760321624318223 3.073221643979557 >500 0.0011002010151917425 0.6574131145614501 >1k 0.0011002010151917425 1.161922280005799 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1503 0.27711198790515873 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1338 0.24669051218702753 No Hit CTGTAGGACGTGGAATATGGCAAGA 1327 0.24466241380581877 No Hit GTCCTACAGTGTGCATTTCTCATTT 1066 0.19654117039713853 No Hit CTTTAGGACGTGAAATATGGCGAGG 1058 0.19506618975625944 No Hit GTACATGGGAAGCAGTGGTATCAAC 872 0.16077288985582064 No Hit GTATCAACGCAGAGTACTTTTTTTT 773 0.14252000442494192 No Hit CCCATGTACTCTGCGTTGATACCAC 726 0.13385449315977727 No Hit CTGTAGGACCTGGAATATGGCGAGA 652 0.12021092223164571 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.8437258010988605E-4 0.0 12 0.0 0.0 0.0 1.8437258010988605E-4 0.0 13 0.0 0.0 0.0 1.8437258010988605E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 30 7.569144E-4 19.053558 4 GTTCTAG 40 0.0051186047 14.314043 1 CCTGTAC 90 3.443529E-8 13.774943 3 GTCCTAT 50 0.0014475749 13.359773 1 CCAACGA 50 0.0015210526 13.27231 19 GTGTAGG 95 6.933078E-8 13.058425 1 GATATAC 155 0.0 12.928812 1 TTCTAGA 60 3.9543078E-4 12.715333 2 TAGGACC 245 0.0 12.443141 4 ACTGTTC 180 0.0 12.155086 8 ATAAGAC 55 0.0029772248 12.137363 3 CTGTACT 110 3.6470738E-8 12.124991 4 AGAGGTC 55 0.0030125822 12.118255 5 GGCGAGG 260 0.0 12.03259 19 TGTACTG 135 3.5470293E-10 11.990019 5 GTACATA 120 9.309588E-9 11.928369 1 ACTGGGG 65 7.919484E-4 11.70791 8 TCCAACG 90 7.6099186E-6 11.586937 18 GTCCTAC 900 0.0 11.451234 1 CTCTAAA 75 1.9846116E-4 11.451234 1 >>END_MODULE