FastQCFastQC Report
Thu 26 May 2016
SRR1926882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542380
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16660.30716471846307014No Hit
CTGTAGGACGTGGAATATGGCAAGA14810.27305579114274126No Hit
CTTTAGGACGTGAAATATGGCGAGG13280.2448467863859287No Hit
GTCCTAAAGTGTGTATTTCTCATTT13260.24447804122570893No Hit
GTATCAACGCAGAGTACTTTTTTTT12620.23267819609867618No Hit
GTCCTACAGTGTGCATTTCTCATTT9880.18216010914856742No Hit
GTACATGGGAAGCAGTGGTATCAAC9510.17533832368450164No Hit
CCCATGTACTCTGCGTTGATACCAC8970.1653822043585678No Hit
CTGTAGGACCTGGAATATGGCGAGA8260.15229175117076588No Hit
GGTATCAACGCAGAGTACTTTTTTT8060.14860429956856816No Hit
TATCAACGCAGAGTACTTTTTTTTT7250.1336701205796674No Hit
CTGAAGGACCTGGAATATGGCGAGA6400.11799845127032707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT508.5264146E-515.235384
TAGGACC2850.013.6984794
AATGTCC1352.7284841E-1112.6528358
AATGCCG550.003091324612.07659318
ATTTAGA1501.2732926E-1112.0680431
GGCGAGG3700.012.05327919
TCTTACA951.0013064E-612.03352
AGGACCT5150.011.4635155
CCAGTAC752.0231033E-411.4275933
TAGAAAT1803.6379788E-1211.1091324
TACACTT600.00576658811.1091325
ACTATGC600.00591520611.0712328
CAGATTA600.00591520611.0712329
TTTAGAA1900.011.0307072
TGGCGAG8000.011.03067318
GAAATGT1657.6397555E-1110.9648586
GTTCTAG700.001453328110.8884591
TCCTAGA1053.3385695E-610.8874512
ATGCCGG700.00150347710.84428719
AAATGTC1502.1464075E-910.7568957
Produced by FastQC (version 0.11.5)