##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926882_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 542380 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.976516464471405 31.0 30.0 33.0 26.0 34.0 2 30.22468380102511 31.0 30.0 33.0 26.0 34.0 3 30.324645082783288 31.0 30.0 34.0 26.0 34.0 4 33.924634020428485 35.0 35.0 37.0 30.0 37.0 5 33.928754747593935 37.0 35.0 37.0 30.0 37.0 6 33.90248534237988 37.0 35.0 37.0 30.0 37.0 7 33.915511265164646 37.0 35.0 37.0 30.0 37.0 8 33.956654006416166 37.0 35.0 37.0 30.0 37.0 9 35.512926361591504 39.0 35.0 39.0 30.0 39.0 10 35.37904236881891 39.0 35.0 39.0 30.0 39.0 11 35.44245916147351 39.0 35.0 39.0 30.0 39.0 12 35.328291972417865 39.0 35.0 39.0 30.0 39.0 13 35.358990007006156 39.0 35.0 39.0 30.0 39.0 14 36.44667207492902 40.0 37.0 41.0 30.0 41.0 15 36.453056897378225 40.0 37.0 41.0 30.0 41.0 16 36.439700947675064 40.0 37.0 41.0 30.0 41.0 17 36.41727571075629 40.0 37.0 41.0 30.0 41.0 18 36.385950809395624 40.0 37.0 41.0 30.0 41.0 19 36.45675541133522 40.0 37.0 41.0 30.0 41.0 20 36.42636159150411 40.0 37.0 41.0 30.0 41.0 21 36.36876728492938 40.0 37.0 41.0 30.0 41.0 22 36.293770050518084 40.0 37.0 41.0 30.0 41.0 23 36.22507467089495 40.0 37.0 41.0 30.0 41.0 24 36.16578782403481 40.0 37.0 41.0 29.0 41.0 25 36.05131457649618 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2030467888713261 1101 2 0.0681978929223348 1101 3 0.4738628611010576 1101 4 0.3784947438226638 1101 5 0.23926406674455336 1101 6 0.21480197432642 1101 7 0.290748447667589 1101 8 0.3918577806345027 1101 9 0.3670194936523785 1101 10 0.508213212728748 1101 11 0.521005748980464 1101 12 0.3614939375496036 1101 13 0.46010980238577304 1101 14 0.15877667613970914 1101 15 0.310595561718209 1101 16 0.12315703014797919 1101 17 0.3410129612316197 1101 18 0.4945524665790799 1101 19 0.4084551899959763 1101 20 0.3559609984976859 1101 21 0.2828925987219719 1101 22 0.2787688496100884 1101 23 0.27902093481631596 1101 24 0.4320907407309491 1101 25 0.2923922009801103 1102 1 -0.9119030400519961 1102 2 -0.8286984430168438 1102 3 -0.5732665064911977 1102 4 -0.9086525564659382 1102 5 -0.9155595483749508 1102 6 -0.730453488763672 1102 7 -0.6833160789061168 1102 8 -0.7120506754412901 1102 9 -0.7637553171358817 1102 10 -0.7747077619880187 1102 11 -0.7333272076417146 1102 12 -0.7977225655123377 1102 13 -0.7204593739243919 1102 14 -0.6861226236756721 1102 15 -0.6068424288235406 1102 16 -0.872574614523117 1102 17 -0.8706722452278868 1102 18 -0.8133108339710446 1102 19 -0.9616962105289275 1102 20 -0.8594835362329007 1102 21 -1.0346453292421387 1102 22 -0.7843162590913124 1102 23 -0.8687697009538056 1102 24 -0.8442549119911789 1102 25 -0.7953534785165246 1103 1 -0.7296780639365927 1103 2 -0.7285876429057367 1103 3 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22 0.9300842802922205 1108 23 0.7155119208476535 1108 24 0.621059075766702 1108 25 0.7432616130344059 1109 1 0.60752168744499 1109 2 0.3707818478526228 1109 3 0.5032409044532287 1109 4 0.6493851224460272 1109 5 0.6445542727145863 1109 6 0.6589361823167295 1109 7 0.6420185437209938 1109 8 0.5878882527484421 1109 9 0.3583703411074666 1109 10 0.481942030098395 1109 11 0.5351702162655059 1109 12 0.44056676518253113 1109 13 0.47796413513010094 1109 14 0.6419751989945439 1109 15 0.5565396322303116 1109 16 0.3362939703172998 1109 17 0.41461543592936323 1109 18 0.3144759415870624 1109 19 0.4665997961530479 1109 20 0.4193841205554989 1109 21 0.6294791734267591 1109 22 0.5332511668815698 1109 23 0.588866221245496 1109 24 0.5507764172487484 1109 25 0.5710175087811322 1110 1 0.27774680228431237 1110 2 0.16819833510868065 1110 3 0.22864842283248166 1110 4 0.23393960539623038 1110 5 0.17698211988651025 1110 6 0.21791349224896805 1110 7 0.323583731779685 1110 8 0.21193952615036693 1110 9 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24 -0.05298866420282877 2212 25 -0.0472405206876374 2213 1 0.43117753710081885 2213 2 0.39833307219286596 2213 3 0.44621197736710627 2213 4 0.4872659146269953 2213 5 0.42293312142280826 2213 6 0.36001117630073765 2213 7 0.2235009668148038 2213 8 0.24980148916418443 2213 9 0.1911705053407715 2213 10 0.24545558954255853 2213 11 0.18357363199193344 2213 12 0.20213481256003263 2213 13 0.20761681160273326 2213 14 0.11918714832693666 2213 15 0.10403609400867708 2213 16 0.00504165058986672 2213 17 0.18555892091112725 2213 18 0.19973695425940008 2213 19 0.18349856018866717 2213 20 0.09605796109964615 2213 21 0.32755364670418174 2213 22 0.15903035737996873 2213 23 0.3658800845484649 2213 24 0.3045760118317702 2213 25 0.27369812802770355 2214 1 0.4451269585610689 2214 2 0.3473037543251358 2214 3 0.4405593432791406 2214 4 0.31645805849067443 2214 5 0.4109646536608551 2214 6 0.4067253743294188 2214 7 0.46722496216623455 2214 8 0.2034046883303091 2214 9 0.2849669339430747 2214 10 0.1639339108201483 2214 11 0.3060617789974671 2214 12 0.3852347086958119 2214 13 0.31974817699111924 2214 14 0.5811630878905589 2214 15 0.3148582268437252 2214 16 0.4078829112371807 2214 17 0.24450464352079848 2214 18 0.39734573072409063 2214 19 0.40635585124683615 2214 20 0.2673721707374028 2214 21 0.4157295514405632 2214 22 0.34209073324246475 2214 23 0.4281393350711795 2214 24 0.19873330992948723 2214 25 0.1668130789175848 2215 1 0.5307894628264762 2215 2 0.3864393143403113 2215 3 0.3150597238847226 2215 4 0.35222424496593163 2215 5 0.5379019837902632 2215 6 0.2916930049582618 2215 7 0.19931323402600043 2215 8 0.29578804286142457 2215 9 0.16177003796651945 2215 10 0.169048820298066 2215 11 0.04378635418675714 2215 12 0.2333158122849639 2215 13 0.35479013905464996 2215 14 0.40840036862475415 2215 15 0.34042561550764816 2215 16 0.33740645032024474 2215 17 0.2171797877094619 2215 18 0.3474852065621974 2215 19 0.2778853607070957 2215 20 0.4108008185257148 2215 21 0.4730542402149638 2215 22 0.2753212990920062 2215 23 0.4664671522086934 2215 24 0.49106106502470936 2215 25 0.48709685080240916 2216 1 -0.0493583766535437 2216 2 0.30858931708177195 2216 3 -1.9036165993683447 2216 4 -1.2746879236288962 2216 5 -0.42358049827809197 2216 6 -0.16481746492804206 2216 7 0.013549388405103002 2216 8 0.07322133814660958 2216 9 0.09496064921248859 2216 10 -0.05158390875094909 2216 11 0.03800187284613088 2216 12 0.03660107868014251 2216 13 0.060486893664041474 2216 14 -0.1805301129252186 2216 15 -0.07945645185252914 2216 16 0.03384953012664482 2216 17 -0.06605792680980471 2216 18 0.13374351194094203 2216 19 0.0789226296144463 2216 20 0.19358993044209427 2216 21 0.10722380202990678 2216 22 0.20729071436343105 2216 23 -0.1374516407512374 2216 24 -0.15498151234677948 2216 25 -0.29604366648624136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12206.0 3 11.0 4 158.0 5 1168.0 6 1753.0 7 1212.0 8 979.0 9 828.0 10 750.0 11 692.0 12 656.0 13 592.0 14 566.0 15 604.0 16 650.0 17 751.0 18 703.0 19 937.0 20 1229.0 21 1556.0 22 1933.0 23 2301.0 24 2787.0 25 3385.0 26 4008.0 27 4584.0 28 5257.0 29 6515.0 30 8413.0 31 10933.0 32 14264.0 33 19098.0 34 25770.0 35 36739.0 36 54212.0 37 94420.0 38 219760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.259524793998644 19.991730411412032 10.724991878082637 28.023752916506687 2 13.562493771476872 21.30745466557906 41.17469023478302 23.955361328161047 3 18.338103070579816 25.55374436666986 29.47642299210043 26.631729570649888 4 11.918581068623475 18.00582617353147 38.4166082820163 31.658984475828756 5 12.398637062604404 39.168224678164606 35.794253979843575 12.638884279387415 6 30.775478938545625 38.67014761335588 18.213911698423214 12.34046174967528 7 27.252157139722165 32.033179104835426 22.328217176346058 18.38644657909635 8 24.238390081462114 39.283251404105926 20.172659854186872 16.30569866024509 9 26.199023854171855 15.764736685861374 20.24866457571584 37.78757488425094 10 14.767941825047384 30.034626192003895 34.36806277702797 20.82936920592075 11 33.525825050105 22.718816434072508 25.353592902144513 18.401765613677977 12 23.41480772670033 25.32620178878607 32.094162937724356 19.164827546789237 13 29.204540007153685 22.829834543436498 25.693520017404836 22.272105432004984 14 20.30642722814263 23.570006268667722 27.47778310409676 28.64578339909289 15 22.141177988992652 32.90440939219898 23.181252477575065 21.7731601412333 16 20.969508350264945 28.775319057926392 29.353882347735343 20.901290244073323 17 20.7590619123124 29.833142815000553 28.418083262657177 20.98971201002987 18 20.643313992614022 28.010671504612088 31.264669170450922 20.081345332322968 19 23.299205905833375 27.300651389526514 27.58108260091191 21.819060103728205 20 23.083574709104553 28.38523757460217 28.491436768152038 20.03975094814124 21 22.03446711683637 27.444801351246696 27.407369162514705 23.11336236940223 22 22.53973229101368 29.49592536597957 27.16453409049006 20.799808252516687 23 21.14609894557127 29.129262010512946 28.21753792090033 21.507101123015456 24 22.838047125631476 27.63431542461005 28.91717983701464 20.610457612743833 25 22.01276299947082 28.300411727503878 28.442386728840653 21.24443854418465 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 167.0 1 167.0 2 185.0 3 203.0 4 203.0 5 203.0 6 350.5 7 498.0 8 498.0 9 498.0 10 779.5 11 1061.0 12 1061.0 13 1061.0 14 1773.0 15 2485.0 16 2485.0 17 2485.0 18 4474.0 19 6463.0 20 6463.0 21 6463.0 22 10364.5 23 14266.0 24 14266.0 25 14266.0 26 21006.0 27 27746.0 28 27746.0 29 27746.0 30 37106.0 31 46466.0 32 46466.0 33 46466.0 34 52114.5 35 57763.0 36 57763.0 37 57763.0 38 61086.0 39 64409.0 40 64409.0 41 64409.0 42 66971.0 43 69533.0 44 69533.0 45 69533.0 46 71580.0 47 73627.0 48 73627.0 49 73627.0 50 69108.0 51 64589.0 52 64589.0 53 64589.0 54 55077.5 55 45566.0 56 45566.0 57 45566.0 58 38462.5 59 31359.0 60 31359.0 61 31359.0 62 25292.0 63 19225.0 64 19225.0 65 19225.0 66 14598.0 67 9971.0 68 9971.0 69 9971.0 70 7175.5 71 4380.0 72 4380.0 73 4380.0 74 3059.0 75 1738.0 76 1738.0 77 1738.0 78 1168.5 79 599.0 80 599.0 81 599.0 82 391.5 83 184.0 84 184.0 85 184.0 86 121.0 87 58.0 88 58.0 89 58.0 90 41.0 91 24.0 92 24.0 93 24.0 94 12.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11726096094988753 2 0.09550499649692099 3 0.013274825767911795 4 0.0 5 0.0040561967624174935 6 0.3528891183303219 7 0.02046535639219735 8 0.0077436483646152144 9 0.007928020944725101 10 0.0029499612817581768 11 0.003503079022087835 12 1.8437258010988605E-4 13 3.687451602197721E-4 14 0.0 15 0.002765588701648291 16 3.687451602197721E-4 17 0.0 18 1.8437258010988605E-4 19 1.8437258010988605E-4 20 5.531177403296581E-4 21 0.01216859028725248 22 0.0 23 1.8437258010988605E-4 24 0.0 25 0.005715549983406468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 542380.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.03258338609831 #Duplication Level Percentage of deduplicated Percentage of total 1 92.06878780264938 74.60571724875177 2 5.965245224187803 9.667584620950457 3 0.9961801614343874 2.421691559970266 4 0.32798422492773216 1.0630963622312513 5 0.1594100931729349 0.6458705833810774 6 0.10029656604919192 0.4876373911032276 7 0.06573360692142795 0.37285947888907334 8 0.04352461107460574 0.282152934100039 9 0.03598914432875847 0.26246640049330033 >10 0.18948209003962674 2.8970011367411113 >50 0.024594141660108926 1.4133170004473679 >100 0.01981193019208086 3.406241148420471 >500 0.0018217866843248868 1.1710280257787957 >1k 0.0011386166777030543 1.3033361087418271 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1666 0.30716471846307014 No Hit CTGTAGGACGTGGAATATGGCAAGA 1481 0.27305579114274126 No Hit CTTTAGGACGTGAAATATGGCGAGG 1328 0.2448467863859287 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1326 0.24447804122570893 No Hit GTATCAACGCAGAGTACTTTTTTTT 1262 0.23267819609867618 No Hit GTCCTACAGTGTGCATTTCTCATTT 988 0.18216010914856742 No Hit GTACATGGGAAGCAGTGGTATCAAC 951 0.17533832368450164 No Hit CCCATGTACTCTGCGTTGATACCAC 897 0.1653822043585678 No Hit CTGTAGGACCTGGAATATGGCGAGA 826 0.15229175117076588 No Hit GGTATCAACGCAGAGTACTTTTTTT 806 0.14860429956856816 No Hit TATCAACGCAGAGTACTTTTTTTTT 725 0.1336701205796674 No Hit CTGAAGGACCTGGAATATGGCGAGA 640 0.11799845127032707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8437258010988605E-4 0.0 10 0.0 0.0 0.0 1.8437258010988605E-4 0.0 11 0.0 0.0 0.0 1.8437258010988605E-4 0.0 12 0.0 0.0 0.0 1.8437258010988605E-4 0.0 13 0.0 0.0 0.0 1.8437258010988605E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATAGT 50 8.5264146E-5 15.23538 4 TAGGACC 285 0.0 13.698479 4 AATGTCC 135 2.7284841E-11 12.652835 8 AATGCCG 55 0.0030913246 12.076593 18 ATTTAGA 150 1.2732926E-11 12.068043 1 GGCGAGG 370 0.0 12.053279 19 TCTTACA 95 1.0013064E-6 12.0335 2 AGGACCT 515 0.0 11.463515 5 CCAGTAC 75 2.0231033E-4 11.427593 3 TAGAAAT 180 3.6379788E-12 11.109132 4 TACACTT 60 0.005766588 11.109132 5 ACTATGC 60 0.005915206 11.071232 8 CAGATTA 60 0.005915206 11.071232 9 TTTAGAA 190 0.0 11.030707 2 TGGCGAG 800 0.0 11.030673 18 GAAATGT 165 7.6397555E-11 10.964858 6 GTTCTAG 70 0.0014533281 10.888459 1 TCCTAGA 105 3.3385695E-6 10.887451 2 ATGCCGG 70 0.001503477 10.844287 19 AAATGTC 150 2.1464075E-9 10.756895 7 >>END_MODULE