FastQCFastQC Report
Thu 26 May 2016
SRR1926881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549186
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7700.14020750711052357No Hit
GTATCAACGCAGAGTACTTTTTTTT6550.1192674248797311No Hit
CTGTAGGACGTGGAATATGGCAAGA6390.11635402213457735No Hit
GTCCTAAAGTGTGTATTTCTCATTT6340.11544358377671682No Hit
GTCCTACAGTGTGCATTTCTCATTT6320.1150794084335726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCACG250.005930328719.0533644
TGCGAGT453.4481633E-516.9363234
TAGATTG400.005194982514.282185
TCCCGTT551.9905793E-413.79009211
CTAGGAT500.001457986713.3471283
GTATTAT603.9521445E-412.716211
GCCAGTA603.964388E-412.7115512
GTAGGAC4600.012.6424673
TCCAACG905.512029E-712.6420718
CCAACGA604.1520564E-412.6420719
TAGGACC2150.012.4068424
GGACCTG3150.012.3930026
TGTTAAG853.7776972E-612.3376822
CTTACAC853.7776972E-612.3376823
GTATTGG550.002975766312.1382011
ACCTAGG550.002975766312.1382011
CAGTACC550.003000372812.1248674
AGACAGT1103.6759957E-812.1182136
AGGACCT3600.011.9018155
ATACTGA1209.624273E-911.9018156
Produced by FastQC (version 0.11.5)