##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926881_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 549186 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.56501076138139 31.0 28.0 31.0 16.0 34.0 2 27.501873682140477 31.0 28.0 31.0 16.0 34.0 3 26.95027914040052 31.0 27.0 31.0 10.0 34.0 4 31.134286016030998 35.0 32.0 37.0 17.0 37.0 5 30.57621461581322 35.0 30.0 37.0 10.0 37.0 6 30.101368206764192 35.0 30.0 37.0 10.0 37.0 7 30.210589854803292 35.0 30.0 37.0 10.0 37.0 8 30.256066250778424 35.0 30.0 37.0 10.0 37.0 9 32.355843011293075 37.0 32.0 39.0 11.0 39.0 10 32.59808880779918 37.0 32.0 39.0 11.0 39.0 11 32.782971525129916 37.0 33.0 39.0 13.0 39.0 12 32.685556441715555 37.0 33.0 39.0 10.0 39.0 13 32.76712079331957 37.0 33.0 39.0 10.0 39.0 14 33.67768843342693 38.0 33.0 40.0 10.0 41.0 15 33.29145681062518 38.0 33.0 40.0 10.0 41.0 16 33.32270487594367 38.0 33.0 40.0 10.0 41.0 17 33.52690891610493 38.0 33.0 40.0 10.0 41.0 18 33.54855914025485 38.0 33.0 40.0 8.0 41.0 19 32.95810526852469 38.0 32.0 40.0 2.0 41.0 20 33.275027040019225 39.0 33.0 40.0 2.0 41.0 21 33.24732786341968 39.0 33.0 40.0 2.0 41.0 22 33.25132286693397 39.0 33.0 40.0 2.0 41.0 23 33.215244015688675 39.0 33.0 40.0 2.0 41.0 24 31.92183158347081 38.0 31.0 40.0 2.0 41.0 25 32.47581693633851 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.279628801402243 1101 2 -0.6739154030251946 1101 3 -1.388096114571475 1101 4 -0.7751920053115313 1101 5 -0.4820588285084675 1101 6 -2.8109452085172677 1101 7 -0.8143829963682201 1101 8 -0.3267325543989621 1101 9 -0.5653206442988363 1101 10 -8.498007010691325 1101 11 -3.984088319804293 1101 12 -1.687405078846421 1101 13 -1.033963534341865 1101 14 -0.992617423899766 1101 15 -2.3201944669957584 1101 16 -0.6943750797819277 1101 17 -0.6804665889205239 1101 18 -0.41523557364354957 1101 19 -1.1819996880754324 1101 20 -0.6823155305106212 1101 21 -0.6948023311681553 1101 22 -0.3901080354404982 1101 23 -0.47909835646257193 1101 24 -0.5039528086387293 1101 25 -0.43823893747023845 1102 1 -1.3153676227081377 1102 2 -1.4868693382555165 1102 3 -1.9566097720031905 1102 4 -1.6511606254684317 1102 5 -1.79340477177875 1102 6 -2.8075147256696837 1102 7 -1.6386873748463273 1102 8 -1.0950827126481713 1102 9 -1.1108664165699729 1102 10 -1.0939840308062223 1102 11 -1.4401085397031927 1102 12 -1.2628157017933042 1102 13 -1.553980934254863 1102 14 -1.5889649421621783 1102 15 -1.0676272298319418 1102 16 -1.3007635478395834 1102 17 -1.407476953868695 1102 18 -1.9498269006869187 1102 19 -1.0308829436591544 1102 20 -1.2961109615334614 1102 21 -1.4903593533830417 1102 22 -1.7293118394214808 1102 23 -1.8519664921218926 1102 24 -4.919650230151621 1102 25 -3.4014021216543178 1103 1 -0.6282510582909566 1103 2 -0.8414705652493844 1103 3 -0.6253214284449058 1103 4 -0.7419581714807002 1103 5 -1.3430980233124927 1103 6 -0.6602754618660001 1103 7 -1.1073990312880468 1103 8 -0.4177820991512391 1103 9 -1.0740566006190555 1103 10 -0.6399963007448726 1103 11 -0.8793723433841727 1103 12 -1.0996255177442258 1103 13 -0.8987662103284855 1103 14 -1.0760815065793494 1103 15 -0.6958480887276508 1103 16 -0.9989230570420418 1103 17 -1.041832782089557 1103 18 -1.3694588025274115 1103 19 -3.0247479743340016 1103 20 -1.4740250719629095 1103 21 -1.3136722368186255 1103 22 -1.2556922075196404 1103 23 -1.514543179238455 1103 24 0.15887737721034156 1103 25 -0.6529358639856078 1104 1 0.034266138213968844 1104 2 0.013721849697631683 1104 3 -1.1830979668349428 1104 4 -0.28977746874785026 1104 5 -0.4427745431117103 1104 6 -1.5586506245356446 1104 7 -0.6916935098156536 1104 8 0.22293922451487092 1104 9 0.1152202166332259 1104 10 0.22362890840180683 1104 11 -0.019807368481778553 1104 12 0.2700565792998333 1104 13 -0.03287292161311228 1104 14 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0.6476104030374152 2215 4 0.750409611053378 2215 5 1.124907421226201 2215 6 1.8198079158521594 2215 7 1.081982803153263 2215 8 -0.0599877948751697 2215 9 0.1996945418792535 2215 10 -0.10431418815865356 2215 11 0.10719253408083063 2215 12 -0.30749774204869595 2215 13 0.16669055885068929 2215 14 0.3902590138080768 2215 15 0.18574897595583906 2215 16 0.2126485603410302 2215 17 -0.1577633181925009 2215 18 -0.8062423163590324 2215 19 0.5030961067028272 2215 20 -3.513334904352657 2215 21 -1.860010653036511 2215 22 -1.0007273537265462 2215 23 -0.8424188228184377 2215 24 -0.9286613362214915 2215 25 -0.6502898707847038 2216 1 -0.08789836395053285 2216 2 -0.4188322628564798 2216 3 0.5418803881434116 2216 4 -0.34808915705954036 2216 5 0.15977956944239935 2216 6 -1.0253605812456001 2216 7 -9.28853333665548 2216 8 -10.444420082844434 2216 9 -5.194217012804007 2216 10 -2.8809598361869355 2216 11 -2.1418852905596992 2216 12 -1.6283416890984341 2216 13 -1.4768556055957944 2216 14 -1.5465632743003823 2216 15 -2.6507441129488285 2216 16 -1.4729105215203013 2216 17 -1.7647287000775016 2216 18 -1.3555873978584572 2216 19 -0.931269367051712 2216 20 -2.125032959780615 2216 21 -4.297915192227226 2216 22 -3.035195832728789 2216 23 -2.7557656581909953 2216 24 -3.012213903696889 2216 25 -2.409859615081139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38591.0 3 1.0 4 289.0 5 1687.0 6 1907.0 7 1489.0 8 1208.0 9 1124.0 10 1123.0 11 1088.0 12 1125.0 13 1137.0 14 1140.0 15 1121.0 16 1236.0 17 1364.0 18 1467.0 19 1903.0 20 2568.0 21 3197.0 22 4041.0 23 4846.0 24 5574.0 25 6422.0 26 7199.0 27 7977.0 28 9002.0 29 10472.0 30 12684.0 31 15275.0 32 19670.0 33 25088.0 34 33999.0 35 47353.0 36 67251.0 37 101203.0 38 106365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.228943359392815 18.64040649145693 12.45108145337116 25.679568695779093 2 14.6236974704128 19.99660923018587 42.02239366472943 23.357299634671897 3 19.852516247823747 24.27575268670185 28.988669820521935 26.883061244952465 4 11.725989055743549 16.794677658300852 39.21874117361935 32.26059211233625 5 13.554552607422302 37.79505616995235 35.90396468068366 12.746426541941688 6 31.351425188962757 37.249430835078776 18.42965121573627 12.9694927602222 7 28.62173515981735 30.849315068493148 22.423013698630136 18.10593607305936 8 24.360030316035537 38.30668505108982 20.62805329047693 16.705231342397706 9 26.091390897335494 15.737155553936658 20.222922396051505 37.948531152676345 10 14.236608891277221 29.566547958430867 35.104970262220725 21.09187288807119 11 34.22018816450371 22.822503979282235 24.32570743807071 18.63160041814334 12 22.88876423729616 25.038154314624666 32.16235708067588 19.910724367403283 13 28.747352490707538 22.216131458511278 25.6847100988707 23.351805951910485 14 20.579145305371664 22.47029148492492 27.86016736935083 29.090395840352585 15 22.93868363494645 31.138602411569416 23.3712072234171 22.551506730067036 16 21.819503053328063 28.695455795495317 27.96914071535115 21.515900435825472 17 21.572305624509404 28.993277834337132 27.7592113338882 21.675205207265257 18 21.34685359004338 28.30712690902601 29.37942313085729 20.96659637007332 19 23.60921893327311 27.222772673956914 27.144636859705233 22.023371533064743 20 23.059789834089127 27.809284452457707 28.35017938771422 20.78074632573895 21 22.695849177557296 27.240591809082566 27.185228083978032 22.878330929382113 22 22.58528625439817 28.391052865457883 27.552395591267036 21.47126528887691 23 22.188610933038056 27.97675418147911 27.70011364298619 22.13452124249665 24 23.12109357926483 27.56421463642059 27.94647930416539 21.36821248014919 25 22.653697518475756 27.67757303796408 27.87098113633632 21.797748307223845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 172.0 2 198.5 3 225.0 4 225.0 5 225.0 6 383.5 7 542.0 8 542.0 9 542.0 10 820.0 11 1098.0 12 1098.0 13 1098.0 14 1883.5 15 2669.0 16 2669.0 17 2669.0 18 4621.0 19 6573.0 20 6573.0 21 6573.0 22 10227.0 23 13881.0 24 13881.0 25 13881.0 26 19746.5 27 25612.0 28 25612.0 29 25612.0 30 33416.0 31 41220.0 32 41220.0 33 41220.0 34 47382.0 35 53544.0 36 53544.0 37 53544.0 38 58636.5 39 63729.0 40 63729.0 41 63729.0 42 66894.0 43 70059.0 44 70059.0 45 70059.0 46 71288.0 47 72517.0 48 72517.0 49 72517.0 50 69593.5 51 66670.0 52 66670.0 53 66670.0 54 59000.5 55 51331.0 56 51331.0 57 51331.0 58 43775.0 59 36219.0 60 36219.0 61 36219.0 62 29221.0 63 22223.0 64 22223.0 65 22223.0 66 17079.0 67 11935.0 68 11935.0 69 11935.0 70 8688.0 71 5441.0 72 5441.0 73 5441.0 74 3830.5 75 2220.0 76 2220.0 77 2220.0 78 1529.5 79 839.0 80 839.0 81 839.0 82 581.0 83 323.0 84 323.0 85 323.0 86 210.5 87 98.0 88 98.0 89 98.0 90 66.0 91 34.0 92 34.0 93 34.0 94 21.0 95 8.0 96 8.0 97 8.0 98 6.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19683677296944932 2 0.11617193446300525 3 0.11853907419344266 4 0.07374550698670396 5 0.02512809867695098 6 0.024763923333806764 7 0.306999814270575 8 0.2951641156183879 9 0.02112216990236459 10 0.040241375417435984 11 0.016752065784633983 12 0.018208767157210856 13 0.015477452083629224 14 0.018390854828782964 15 0.015113276740485009 16 0.020575906887648265 17 0.019483380858215615 18 0.015295364412057116 19 0.02585644936323941 20 0.01748041647092242 21 0.01638789044148977 22 0.017116241127778202 23 0.017844591814066637 24 0.01638789044148977 25 0.01602371509834555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 549186.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.40531590562527 #Duplication Level Percentage of deduplicated Percentage of total 1 95.05654699591854 84.98560613068011 2 3.760834063615908 6.724771150524333 3 0.5711624325059881 1.5319487313486968 4 0.20734796429788 0.7415204100176108 5 0.11463553918232514 0.5124513297303731 6 0.06898463327714266 0.370055575846599 7 0.04248805476995778 0.26590605712465093 8 0.027835273845506055 0.1990897161181646 9 0.018598996523466742 0.1496564243737353 >10 0.11178680884934805 1.81229897933253 >50 0.00999158926785918 0.6437232587506398 >100 0.008564191952883049 1.3620065019766716 >500 0.0012234559932407143 0.7009657341759289 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 770 0.14020750711052357 No Hit GTATCAACGCAGAGTACTTTTTTTT 655 0.1192674248797311 No Hit CTGTAGGACGTGGAATATGGCAAGA 639 0.11635402213457735 No Hit GTCCTAAAGTGTGTATTTCTCATTT 634 0.11544358377671682 No Hit GTCCTACAGTGTGCATTTCTCATTT 632 0.1150794084335726 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.8208767157210855E-4 13 0.0 0.0 0.0 0.0 1.8208767157210855E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCACG 25 0.0059303287 19.053364 4 TGCGAGT 45 3.4481633E-5 16.936323 4 TAGATTG 40 0.0051949825 14.28218 5 TCCCGTT 55 1.9905793E-4 13.790092 11 CTAGGAT 50 0.0014579867 13.347128 3 GTATTAT 60 3.9521445E-4 12.71621 1 GCCAGTA 60 3.964388E-4 12.711551 2 GTAGGAC 460 0.0 12.642467 3 TCCAACG 90 5.512029E-7 12.64207 18 CCAACGA 60 4.1520564E-4 12.64207 19 TAGGACC 215 0.0 12.406842 4 GGACCTG 315 0.0 12.393002 6 TGTTAAG 85 3.7776972E-6 12.337682 2 CTTACAC 85 3.7776972E-6 12.337682 3 GTATTGG 55 0.0029757663 12.138201 1 ACCTAGG 55 0.0029757663 12.138201 1 CAGTACC 55 0.0030003728 12.124867 4 AGACAGT 110 3.6759957E-8 12.118213 6 AGGACCT 360 0.0 11.901815 5 ATACTGA 120 9.624273E-9 11.901815 6 >>END_MODULE