FastQCFastQC Report
Thu 26 May 2016
SRR1926881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549186
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12230.22269322233268873No Hit
GTCCTACAGTGGACATTTCTAAATT8120.14785518931655214No Hit
CTGTAGGACGTGGAATATGGCAAGA8070.14694475095869158No Hit
GGTATCAACGCAGAGTACTTTTTTT7570.13784036738008618No Hit
GTCCTACAGTGTGCATTTCTCATTT6640.12090621392388007No Hit
CTTTAGGACGTGAAATATGGCGAGG6550.1192674248797311No Hit
GTCCTAAAGTGTGTATTTCTCATTT6350.11562567144828892No Hit
TATCAACGCAGAGTACTTTTTTTTT5950.10834216458540458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG307.787277E-418.97332218
GTCTAGG400.005142076414.3042281
TTTAGAA1152.3646862E-1114.0943412
ACTGTAC707.042754E-613.60812953
TAGGACC2400.013.0966264
CTCTTAG701.0504864E-412.2607681
GAACTGC550.003004779612.1224986
GGACCTG3500.011.9740586
ATAGGAC907.2155253E-611.6425113
TAAGACA907.222876E-611.6414464
GGTATCA3100.011.3818591
CTGTAGG5200.011.37002851
GTAGGAC5050.011.3176523
CTACACT1354.5056368E-911.2886754
TAGGACA1354.5056368E-911.2886754
AGGACCT3800.011.2793915
GTCCAAG855.106532E-511.2190031
TGTAGGG855.1160678E-511.2169492
CTGCGTT855.384619E-511.16077811
CTATTAG600.00570382111.125511
Produced by FastQC (version 0.11.5)