##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926881_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 549186 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.927243229069934 31.0 30.0 33.0 26.0 34.0 2 30.244669383414728 31.0 30.0 34.0 26.0 34.0 3 30.298204979733644 31.0 30.0 34.0 26.0 34.0 4 33.88929251656087 35.0 35.0 37.0 30.0 37.0 5 33.85894396433995 37.0 35.0 37.0 30.0 37.0 6 33.830565600725436 37.0 35.0 37.0 30.0 37.0 7 33.82411423452164 37.0 35.0 37.0 29.0 37.0 8 33.84936979456869 37.0 35.0 37.0 30.0 37.0 9 35.41104653068359 39.0 35.0 39.0 30.0 39.0 10 35.29006019818422 39.0 35.0 39.0 30.0 39.0 11 35.3324957300441 39.0 35.0 39.0 30.0 39.0 12 35.22166624786502 39.0 35.0 39.0 29.0 39.0 13 35.24187797940952 39.0 35.0 39.0 30.0 39.0 14 36.334507798814975 40.0 36.0 41.0 30.0 41.0 15 36.33079867294505 40.0 36.0 41.0 29.0 41.0 16 36.30688509903749 40.0 36.0 41.0 29.0 41.0 17 36.288654481359686 40.0 36.0 41.0 29.0 41.0 18 36.2638122603271 40.0 36.0 41.0 29.0 41.0 19 36.34080803225137 40.0 37.0 41.0 29.0 41.0 20 36.29370195161567 40.0 37.0 41.0 29.0 41.0 21 36.2373694886614 40.0 37.0 41.0 29.0 41.0 22 36.154535985986534 40.0 37.0 41.0 29.0 41.0 23 36.07813564074831 40.0 37.0 41.0 29.0 41.0 24 36.02692166224194 40.0 36.0 41.0 29.0 41.0 25 35.90414722880772 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01722798144136206 1101 2 -0.04207484429563024 1101 3 0.39509792052299275 1101 4 0.2051154431382045 1101 5 0.07266115760970848 1101 6 -0.12186030912184975 1101 7 0.009965781556147135 1101 8 -0.13474071865343973 1101 9 0.15683463506435658 1101 10 0.16922694897327517 1101 11 0.14661296779770794 1101 12 0.10990764693057287 1101 13 0.13795300051497605 1101 14 0.45387576063603774 1101 15 0.16049434053841338 1101 16 0.2398241430322301 1101 17 0.2574579127970438 1101 18 0.02740861549706608 1101 19 0.04193149402860996 1101 20 0.041700314233331426 1101 21 0.021175013863988568 1101 22 0.02158952840824213 1101 23 0.03826613244861221 1101 24 0.03899235059467543 1101 25 0.06243341016958226 1102 1 -0.4053180409910624 1102 2 -0.37881416059904893 1102 3 -0.14469088053300183 1102 4 -0.11848693884988393 1102 5 -0.04900473406083705 1102 6 -0.256660135122722 1102 7 -0.01883557443707673 1102 8 0.022461495335491577 1102 9 0.07296555440975538 1102 10 3.3079345404729565E-4 1102 11 -0.0663739672676158 1102 12 -0.1146037305125418 1102 13 -0.18089290525549018 1102 14 -0.3187448762607801 1102 15 -0.11987375762109309 1102 16 -0.04162694971230252 1102 17 -0.09265083665921026 1102 18 -0.14077804356963242 1102 19 -0.013056731030268054 1102 20 0.009934973060040875 1102 21 -0.13477320639491097 1102 22 -0.05496408882367376 1102 23 -0.29738118931478397 1102 24 -0.2845200318434138 1102 25 -0.22464715442759342 1103 1 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0.4374031382708168 2211 12 0.31145858042014396 2211 13 0.1736560190044969 2211 14 0.2539106890328213 2211 15 0.28952659860293295 2211 16 0.28250157365429374 2211 17 0.09191702967196846 2211 18 0.11903932093801473 2211 19 0.22655905591383885 2211 20 0.28418789211801965 2211 21 0.2550037295130352 2211 22 0.38086752464498375 2211 23 0.32806197033578144 2211 24 0.18573950280361373 2211 25 0.3349115235854683 2212 1 0.06581557042374087 2212 2 0.046793939510280325 2212 3 0.400189101082983 2212 4 0.39178373794656096 2212 5 0.25235665811064223 2212 6 0.27325776352842013 2212 7 -0.0025935739217644027 2212 8 -0.0025226623770961965 2212 9 0.11077256401262048 2212 10 0.0360347829250216 2212 11 0.17756801166039793 2212 12 0.19448451973293146 2212 13 0.287836856657556 2212 14 0.2057511300900643 2212 15 0.15992680364851708 2212 16 0.12749207983443256 2212 17 0.4494116550714651 2212 18 0.020047287377487066 2212 19 0.12833725098960969 2212 20 0.2933050976396103 2212 21 0.2527911202556439 2212 22 0.36349095738009396 2212 23 0.32839543095081325 2212 24 0.3499054328056559 2212 25 0.4465240606584402 2213 1 0.2964305603980897 2213 2 0.3403880231257155 2213 3 0.22971927054399544 2213 4 0.10388002420516784 2213 5 0.22434733750480262 2213 6 0.29207773682668403 2213 7 0.24035471771718875 2213 8 0.18619085400147384 2213 9 0.12004941700806881 2213 10 0.15106726003603654 2213 11 -0.05544588424283603 2213 12 0.10032964966637081 2213 13 0.21590718122865837 2213 14 -0.04769409384949341 2213 15 0.23097892203620773 2213 16 0.1819818716708994 2213 17 0.10234659584261152 2213 18 0.028690349176507368 2213 19 -0.0346520933177743 2213 20 0.09859744164197082 2213 21 0.06074988757552546 2213 22 0.16667316422353906 2213 23 0.26026725261922223 2213 24 0.14017930060705908 2213 25 0.1583803313160388 2214 1 0.2620967712367026 2214 2 0.18241757781798285 2214 3 0.17485074302633663 2214 4 0.15468986819841035 2214 5 0.3346805967069244 2214 6 0.1804633484327951 2214 7 -0.09334217397711342 2214 8 7.63077511791721E-4 2214 9 0.03650630813456246 2214 10 -0.17538764374323534 2214 11 -0.17811607450767042 2214 12 -0.09260746025758948 2214 13 -0.060717356831410996 2214 14 -0.2466263678952103 2214 15 -0.3029857883552509 2214 16 -0.027544855783354194 2214 17 -0.17173461095033105 2214 18 -0.15191456146384752 2214 19 -0.17463113153635135 2214 20 -0.17511705227060048 2214 21 -0.034660523284856026 2214 22 -0.17919622920969402 2214 23 -0.15276856221679935 2214 24 -0.22922359618810617 2214 25 -0.21372677723570632 2215 1 0.26170764960395587 2215 2 0.07650051138064384 2215 3 0.004496055204345595 2215 4 0.09565965601199622 2215 5 0.2291940968099624 2215 6 0.1043325068594072 2215 7 0.20410907932840416 2215 8 0.3655174070655747 2215 9 0.1331224465513401 2215 10 0.12020664745098486 2215 11 -0.03802422798737837 2215 12 0.06572359936206595 2215 13 0.20898998455835738 2215 14 0.07798050988721172 2215 15 0.20926175747302977 2215 16 0.19909662158945451 2215 17 0.25565103276392165 2215 18 0.04823556120157235 2215 19 -0.039965114173234895 2215 20 -0.0918450228631329 2215 21 -0.09735055106445856 2215 22 0.014797022177845065 2215 23 -0.2480699760637748 2215 24 -0.20423807312459274 2215 25 -0.13705715186313228 2216 1 -0.13912544013588501 2216 2 -0.029841967148328763 2216 3 -1.8962604765811264 2216 4 -1.4835534674133442 2216 5 -0.46892744938232056 2216 6 -0.2653101762880965 2216 7 -0.3096052342251383 2216 8 -0.248268592572515 2216 9 -0.29682702519877324 2216 10 -0.11290188388905875 2216 11 -0.10232594984536547 2216 12 0.1465374800151764 2216 13 -0.1102079664723945 2216 14 -0.15409796897583306 2216 15 -0.2908043607602906 2216 16 -0.14669825877134457 2216 17 -0.1502279221504068 2216 18 0.004914198748373622 2216 19 -0.0520682307779623 2216 20 -0.2194079743810704 2216 21 -0.0750130279230774 2216 22 -0.17701545259283336 2216 23 -0.2807915417435396 2216 24 -0.4403602039412533 2216 25 -0.227649571233691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12435.0 3 14.0 4 207.0 5 1369.0 6 1827.0 7 1308.0 8 1034.0 9 865.0 10 830.0 11 750.0 12 657.0 13 626.0 14 673.0 15 629.0 16 687.0 17 725.0 18 799.0 19 1003.0 20 1227.0 21 1555.0 22 2139.0 23 2578.0 24 2981.0 25 3659.0 26 4374.0 27 4666.0 28 5516.0 29 6820.0 30 8693.0 31 11516.0 32 14872.0 33 19711.0 34 26596.0 35 37289.0 36 54926.0 37 94675.0 38 218955.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87726618941105 19.331951516079577 10.445481111214413 28.345301183294957 2 14.130710162599403 20.815676324936344 40.34674263509092 24.70687087737333 3 18.61709005414285 24.55131368796018 29.223661134614087 27.607935123282886 4 11.925467874272105 17.496076010677623 37.96710039950035 32.61135571554992 5 12.791447239035902 38.578108952647334 35.54709408619773 13.083349722119033 6 31.60443295295698 38.394744776296676 17.562606780992756 12.438215489753594 7 28.175185672189784 31.784092109432766 21.568023952532027 18.472698265845423 8 24.78160710220806 38.43296152970635 19.679307989984064 17.106123378101525 9 26.055174850262325 15.773036110043762 19.79760601827992 38.37418302141399 10 14.36484813169204 30.138939471192366 34.161628669240294 21.3345837278753 11 34.38700126192501 22.934804183108415 24.07982358543305 18.598370969533526 12 22.832363100689935 25.37305551357385 31.819928220386355 19.974653165349856 13 28.91576137427209 22.12870872467579 25.632672831031105 23.322857070021012 14 20.485773490220073 22.61601715994217 27.66803960771032 29.230169742127437 15 22.623495906534977 31.205204964600995 23.233691693616525 22.9376074352475 16 21.740088386482366 28.870914583405412 27.67003108276172 21.718965947350497 17 21.264965357757404 29.167220517676196 27.68629878820434 21.881515336362064 18 21.115845442295786 28.375760224334464 29.538948978477002 20.96944535489275 19 23.02553233330784 27.41766905929867 27.51745310332019 22.039345504073303 20 22.99477217727618 27.849855328974083 28.124994309667013 21.030378184082725 21 22.625703410974122 27.178889475697037 27.194004844202436 23.001402269126405 22 22.474535039130643 28.41314235978339 27.40565127297491 21.70667132811106 23 22.032608260225135 28.029119460437812 27.659845662489573 22.27842661684748 24 22.419039121607472 27.40205941531542 28.51989766654224 21.659003796534865 25 22.318006093019612 27.71540900259126 27.94940572117424 22.01717918321488 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 143.0 1 143.0 2 148.0 3 153.0 4 153.0 5 153.0 6 279.0 7 405.0 8 405.0 9 405.0 10 723.0 11 1041.0 12 1041.0 13 1041.0 14 1708.0 15 2375.0 16 2375.0 17 2375.0 18 4348.0 19 6321.0 20 6321.0 21 6321.0 22 9919.0 23 13517.0 24 13517.0 25 13517.0 26 19319.5 27 25122.0 28 25122.0 29 25122.0 30 33076.5 31 41031.0 32 41031.0 33 41031.0 34 47167.5 35 53304.0 36 53304.0 37 53304.0 38 58205.5 39 63107.0 40 63107.0 41 63107.0 42 66707.5 43 70308.0 44 70308.0 45 70308.0 46 71895.0 47 73482.0 48 73482.0 49 73482.0 50 70450.5 51 67419.0 52 67419.0 53 67419.0 54 59776.0 55 52133.0 56 52133.0 57 52133.0 58 44119.5 59 36106.0 60 36106.0 61 36106.0 62 29267.0 63 22428.0 64 22428.0 65 22428.0 66 17294.0 67 12160.0 68 12160.0 69 12160.0 70 8755.0 71 5350.0 72 5350.0 73 5350.0 74 3733.5 75 2117.0 76 2117.0 77 2117.0 78 1471.0 79 825.0 80 825.0 81 825.0 82 536.0 83 247.0 84 247.0 85 247.0 86 161.5 87 76.0 88 76.0 89 76.0 90 45.5 91 15.0 92 15.0 93 15.0 94 8.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11526149610514472 2 0.09778107963422228 3 0.015113276740485009 4 0.0 5 0.005462630147163257 6 0.3497904170900205 7 0.018208767157210856 8 0.011107347965898622 9 0.010743172622754404 10 0.004734279460874822 11 0.004552191789302714 12 9.104383578605427E-4 13 0.0014567013725768684 14 0.0 15 0.0032775780882979538 16 0.0012746137010047598 17 1.8208767157210855E-4 18 0.0010925260294326513 19 0.0 20 0.0016387890441489769 21 0.01383866303948025 22 0.0 23 0.0 24 1.8208767157210855E-4 25 0.006008893161879582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 549186.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.80238053715115 #Duplication Level Percentage of deduplicated Percentage of total 1 93.55830456100698 81.21083554915212 2 4.95614523837769 8.604104099580999 3 0.7242350558573991 1.8859598075063666 4 0.26611906784863903 0.9239907438235804 5 0.13972533667439246 0.6064245922346089 6 0.07190034597282925 0.3744672715131808 7 0.0576525071241849 0.35030624036200203 8 0.0345878826442726 0.2401848441009981 9 0.026129148774599435 0.20412650835401552 >10 0.14273980698186162 2.219381326928245 >50 0.01007599998859519 0.6043990473305771 >100 0.010495804024294342 1.5573672887053647 >500 0.0016793286438543309 0.9956072148570383 >1k 2.0991608048179136E-4 0.2228454655509074 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1223 0.22269322233268873 No Hit GTCCTACAGTGGACATTTCTAAATT 812 0.14785518931655214 No Hit CTGTAGGACGTGGAATATGGCAAGA 807 0.14694475095869158 No Hit GGTATCAACGCAGAGTACTTTTTTT 757 0.13784036738008618 No Hit GTCCTACAGTGTGCATTTCTCATTT 664 0.12090621392388007 No Hit CTTTAGGACGTGAAATATGGCGAGG 655 0.1192674248797311 No Hit GTCCTAAAGTGTGTATTTCTCATTT 635 0.11562567144828892 No Hit TATCAACGCAGAGTACTTTTTTTTT 595 0.10834216458540458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8208767157210855E-4 0.0 10 0.0 0.0 0.0 1.8208767157210855E-4 0.0 11 0.0 0.0 0.0 1.8208767157210855E-4 0.0 12 0.0 0.0 0.0 1.8208767157210855E-4 0.0 13 0.0 0.0 0.0 1.8208767157210855E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 30 7.787277E-4 18.973322 18 GTCTAGG 40 0.0051420764 14.304228 1 TTTAGAA 115 2.3646862E-11 14.094341 2 ACTGTAC 70 7.042754E-6 13.6081295 3 TAGGACC 240 0.0 13.096626 4 CTCTTAG 70 1.0504864E-4 12.260768 1 GAACTGC 55 0.0030047796 12.122498 6 GGACCTG 350 0.0 11.974058 6 ATAGGAC 90 7.2155253E-6 11.642511 3 TAAGACA 90 7.222876E-6 11.641446 4 GGTATCA 310 0.0 11.381859 1 CTGTAGG 520 0.0 11.3700285 1 GTAGGAC 505 0.0 11.317652 3 CTACACT 135 4.5056368E-9 11.288675 4 TAGGACA 135 4.5056368E-9 11.288675 4 AGGACCT 380 0.0 11.279391 5 GTCCAAG 85 5.106532E-5 11.219003 1 TGTAGGG 85 5.1160678E-5 11.216949 2 CTGCGTT 85 5.384619E-5 11.160778 11 CTATTAG 60 0.005703821 11.12551 1 >>END_MODULE