FastQCFastQC Report
Thu 26 May 2016
SRR1926880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497123
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8100.16293754262023685No Hit
GTATCAACGCAGAGTACTTTTTTTT6390.1285396169559646No Hit
CTGTAGGACGTGGAATATGGCAAGA6060.12190142077514016No Hit
GTCCTACAGTGTGCATTTCTCATTT5610.11284933507401589No Hit
GTCCTAAAGTGTGTATTTCTCATTT5530.11124007539381602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1450.015.7600884
CTACACG400.005193101214.2825794
TAGTACA853.8289836E-612.3222254
CCTACAC701.05405365E-412.255833
TATACCT550.003011419212.1185524
AGGACCT2650.011.8524465
AGAGTGT1052.6400812E-711.7840346
GTAGGAC4050.011.7682863
CCTGGAC1406.366463E-1011.574953
TGTAGGA4150.011.4847132
GTATAGC751.9835246E-411.4515131
TATCCAA752.0325831E-411.4214485
GTCCTAA2100.011.3606281
CTGTAGG4250.011.2269741
CTACACT855.1836578E-511.2020234
GGACAGC855.2050458E-511.1974986
CTAGGAC600.005719647711.121033
TAGGTCT600.00576720311.1086734
CAAGACC600.00576720311.1086734
TAACAGT600.005784579611.1041866
Produced by FastQC (version 0.11.5)