FastQCFastQC Report
Thu 26 May 2016
SRR1926880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497123
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10830.2178535292070574No Hit
GTCCTACAGTGGACATTTCTAAATT8000.160925968019987No Hit
GGTATCAACGCAGAGTACTTTTTTT7700.1548912442192375No Hit
CTGTAGGACGTGGAATATGGCAAGA7610.15308082707901266No Hit
TATCAACGCAGAGTACTTTTTTTTT6050.12170026331511517No Hit
GTACATGGGAAGCAGTGGTATCAAC6020.12109679093504021No Hit
GTCCTACAGTGTGCATTTCTCATTT5810.11687248427451556No Hit
CTTTAGGACGTGAAATATGGCGAGG5630.11325164999406587No Hit
GTCCTAAAGTGTGTATTTCTCATTT5390.10842387095346626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGT508.509487E-515.2384496
TTAGCAC456.622745E-414.8151593
TAGGACC2650.014.0164994
AATGCCG759.76619E-713.91658818
GTCCTAG707.0155056E-613.6126311
GGGTTAG500.001463219813.3403781
TCCAACG500.001510673113.28401618
ATGCCGG655.4996435E-513.13803819
AGGACCT3050.012.8027955
CTAAGAC603.997179E-412.6987083
GGACCTG3100.012.5962986
TGTAGGA5800.012.4860682
GTATAAT550.002994599512.1276161
TCTAAAT2700.011.948585518
GTATTAT802.7698454E-511.9110521
GCATTTC2800.011.85953513
GTAGGAC5450.011.7084413
GTCCTAC4750.011.6352171
ACAGTCC907.5320804E-611.5971578
GGCGAGG1751.8189894E-1211.38629919
Produced by FastQC (version 0.11.5)