##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926880_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 497123 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.86827404887724 31.0 30.0 33.0 26.0 34.0 2 30.18694970862342 31.0 30.0 34.0 26.0 34.0 3 30.228689881578603 31.0 30.0 34.0 26.0 34.0 4 33.80135700822533 35.0 35.0 37.0 28.0 37.0 5 33.79761950261806 37.0 35.0 37.0 29.0 37.0 6 33.734798027852264 37.0 35.0 37.0 29.0 37.0 7 33.724689865486006 37.0 35.0 37.0 29.0 37.0 8 33.757367894867066 37.0 35.0 37.0 29.0 37.0 9 35.30415209113237 39.0 35.0 39.0 30.0 39.0 10 35.18308346224174 39.0 35.0 39.0 29.0 39.0 11 35.200370934356286 39.0 35.0 39.0 29.0 39.0 12 35.09378765416205 39.0 35.0 39.0 29.0 39.0 13 35.12359315501395 39.0 35.0 39.0 29.0 39.0 14 36.21305994693466 40.0 36.0 41.0 29.0 41.0 15 36.20698499164191 40.0 36.0 41.0 29.0 41.0 16 36.16835672459331 40.0 36.0 41.0 29.0 41.0 17 36.14479112815138 40.0 36.0 41.0 29.0 41.0 18 36.10507661081865 40.0 36.0 41.0 28.0 41.0 19 36.18364469155521 40.0 36.0 41.0 29.0 41.0 20 36.148683525002866 40.0 36.0 41.0 29.0 41.0 21 36.09128525535934 40.0 36.0 41.0 28.0 41.0 22 36.00756352049694 40.0 36.0 41.0 28.0 41.0 23 35.93819034725812 40.0 36.0 41.0 28.0 41.0 24 35.862649686294944 40.0 36.0 41.0 27.0 41.0 25 35.756418431655746 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1938581382551945 1101 2 -0.0726531415731948 1101 3 0.0889419851879012 1101 4 0.028305418977694785 1101 5 -0.028921018237824114 1101 6 0.1541712004703868 1101 7 0.11619055011066592 1101 8 -0.12718227174450902 1101 9 -0.19112067843150982 1101 10 -0.14720477910368146 1101 11 -0.13953980608274463 1101 12 -0.22573473439921798 1101 13 -0.24016461405116019 1101 14 -0.34839748643276636 1101 15 -0.3286061469579664 1101 16 -0.12913741921937572 1101 17 -0.25494190845323317 1101 18 -0.061690284595513845 1101 19 -0.5497756761122332 1101 20 -0.30122577298050857 1101 21 -0.3726653561596791 1101 22 -0.3525462408237132 1101 23 -0.26168983946458013 1101 24 -0.2397835115924991 1101 25 -0.16332993344963143 1102 1 -0.4200147678752515 1102 2 -0.364138575240073 1102 3 -0.13583001200591127 1102 4 -0.2157111227643611 1102 5 -0.4289579417691911 1102 6 -0.28314372032863844 1102 7 -0.46446521180639877 1102 8 -0.5586743516470278 1102 9 -0.3852609140036449 1102 10 -0.40729044169645334 1102 11 -0.2974211338529287 1102 12 -0.4127708825364209 1102 13 -0.3019081432022901 1102 14 -0.34131924546980486 1102 15 -0.35272459764659203 1102 16 -0.0869818234581956 1102 17 -0.07319321000771595 1102 18 -0.15446065715394752 1102 19 -0.1808615971697236 1102 20 -0.2509322309061446 1102 21 -0.36752560059345285 1102 22 -0.16683195510942994 1102 23 -0.3418729801801561 1102 24 -0.47784428772512655 1102 25 -0.5575698625564556 1103 1 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0.7292364983506374 2215 18 0.6640936535983428 2215 19 0.6148870519277594 2215 20 0.8717827318562144 2215 21 0.7449058961937567 2215 22 0.9277855127307859 2215 23 0.8032260860044858 2215 24 1.0278818782666193 2215 25 0.9160571989271773 2216 1 -0.36310594401354024 2216 2 -0.12441263628240762 2216 3 -1.7063660289208684 2216 4 -1.756158529837002 2216 5 -0.6950763787496825 2216 6 -0.46710679149089884 2216 7 -0.5139382334181661 2216 8 -0.4034844618847728 2216 9 -0.48837274963157995 2216 10 -0.6931403053115375 2216 11 -0.5183356390742944 2216 12 -0.6312223892265507 2216 13 -0.679529719456788 2216 14 -0.7020436188255204 2216 15 -0.7544612859949638 2216 16 -0.6591431611601521 2216 17 -0.7851773280250498 2216 18 -0.6024826724226031 2216 19 -0.7037581221790035 2216 20 -0.8254403575100753 2216 21 -0.8081505501552471 2216 22 -0.7202109114823969 2216 23 -1.0815569545494768 2216 24 -1.2399147559530874 2216 25 -1.0281236337203268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11771.0 3 12.0 4 174.0 5 1273.0 6 1679.0 7 1313.0 8 974.0 9 907.0 10 803.0 11 700.0 12 661.0 13 627.0 14 628.0 15 619.0 16 690.0 17 671.0 18 797.0 19 900.0 20 1213.0 21 1509.0 22 1905.0 23 2392.0 24 2882.0 25 3423.0 26 4072.0 27 4461.0 28 5164.0 29 6233.0 30 8069.0 31 10712.0 32 13672.0 33 17760.0 34 24357.0 35 33886.0 36 49631.0 37 85081.0 38 195502.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96643796581772 19.29001375427178 10.326578368135198 28.416969911775308 2 14.257495917541382 20.62895031985132 40.20723385490811 24.906319907699185 3 18.841856843655503 24.173049491285354 29.076306190484814 27.908787474574325 4 11.885790840496215 17.328709393852225 37.66130313825754 33.12419662739403 5 12.779923556628445 38.68859384429692 35.26735063367531 13.264131965399317 6 32.07443963385712 37.9767275222784 17.43094452349955 12.51788832036493 7 28.452291132236805 31.624163774458026 20.992103012926915 18.93144208037825 8 25.045013509082164 38.559527473831814 19.275036061101687 17.12042295598433 9 25.888149225566465 15.79325370720196 19.676989094157197 38.64160797307438 10 14.381124233810699 30.176442306725164 34.0022087977743 21.44022466168983 11 34.48046252635306 23.0470573088498 23.534890644865378 18.937589519931763 12 22.823014919908836 25.48816083005948 31.448681508123776 20.240142741907903 13 28.96837370604162 22.01610080504025 25.47262420592294 23.542901282995185 14 20.399780336053652 22.69257306541842 27.378133781780363 29.529512816747566 15 23.000464692427926 31.157929040862758 22.88660498930805 22.955001277401262 16 21.802626735248502 28.90173177850776 27.56382274666629 21.731818739577445 17 21.4182836406355 29.126451856888245 27.552995039447058 21.902269463029196 18 21.196692951400063 28.420904409398133 29.387270679111683 20.99513196009012 19 23.113394471790684 27.344942800876243 27.420376848385608 22.121285878947464 20 22.98664907195259 27.907804771091026 28.105745304444206 20.999800852512173 21 22.57668637600919 27.0584661316509 27.257839644145438 23.10700784819447 22 22.38520446650024 28.315728702956815 27.5096907606367 21.78937606990624 23 21.9927462619915 28.037930250662313 27.506673398736332 22.462650088609863 24 22.423670648251335 27.314019496220244 28.49179879385744 21.770511061670977 25 22.410737455768544 27.639252385330142 27.809036795339377 22.140973363561933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 155.0 1 155.0 2 168.5 3 182.0 4 182.0 5 182.0 6 292.0 7 402.0 8 402.0 9 402.0 10 678.5 11 955.0 12 955.0 13 955.0 14 1505.0 15 2055.0 16 2055.0 17 2055.0 18 3769.5 19 5484.0 20 5484.0 21 5484.0 22 8709.0 23 11934.0 24 11934.0 25 11934.0 26 17057.5 27 22181.0 28 22181.0 29 22181.0 30 29183.0 31 36185.0 32 36185.0 33 36185.0 34 41564.0 35 46943.0 36 46943.0 37 46943.0 38 51217.0 39 55491.0 40 55491.0 41 55491.0 42 59243.5 43 62996.0 44 62996.0 45 62996.0 46 65156.0 47 67316.0 48 67316.0 49 67316.0 50 65071.0 51 62826.0 52 62826.0 53 62826.0 54 55340.5 55 47855.0 56 47855.0 57 47855.0 58 40789.5 59 33724.0 60 33724.0 61 33724.0 62 27338.0 63 20952.0 64 20952.0 65 20952.0 66 16150.5 67 11349.0 68 11349.0 69 11349.0 70 8218.0 71 5087.0 72 5087.0 73 5087.0 74 3547.5 75 2008.0 76 2008.0 77 2008.0 78 1385.5 79 763.0 80 763.0 81 763.0 82 491.0 83 219.0 84 219.0 85 219.0 86 130.5 87 42.0 88 42.0 89 42.0 90 29.0 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11063660301374106 2 0.0977625255721421 3 0.0172995415621486 4 0.0 5 0.004626621580574627 6 0.33955379252217255 7 0.01971343108244841 8 0.010057873001249187 9 0.010057873001249187 10 0.004023149200499675 11 0.005431251420674562 12 4.023149200499675E-4 13 0.0010057873001249188 14 0.0 15 0.004023149200499675 16 8.04629840099935E-4 17 2.0115746002498375E-4 18 6.034723800749512E-4 19 0.0 20 8.04629840099935E-4 21 0.013276392361648928 22 0.0 23 0.0 24 2.0115746002498375E-4 25 0.004425464120549643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 497123.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.53991720400167 #Duplication Level Percentage of deduplicated Percentage of total 1 93.41553599716133 80.84172750761779 2 5.158255512239554 8.927900098925926 3 0.7287900065876253 1.8920828048759077 4 0.24812805107123095 0.8589192398277848 5 0.11981859600182937 0.5184545688749018 6 0.07006108105464041 0.36378480922148587 7 0.04737327517226104 0.28697755177629003 8 0.030333062514966272 0.210001657487119 9 0.02511107182274929 0.19557990688000162 >10 0.13165855899526022 2.124697217352703 >50 0.011884808496321812 0.725402205532558 >100 0.010952660847563899 1.7831536538001835 >500 0.0018642826974555582 1.0529116971821895 >1k 2.3303533718194478E-4 0.2184070806451468 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1083 0.2178535292070574 No Hit GTCCTACAGTGGACATTTCTAAATT 800 0.160925968019987 No Hit GGTATCAACGCAGAGTACTTTTTTT 770 0.1548912442192375 No Hit CTGTAGGACGTGGAATATGGCAAGA 761 0.15308082707901266 No Hit TATCAACGCAGAGTACTTTTTTTTT 605 0.12170026331511517 No Hit GTACATGGGAAGCAGTGGTATCAAC 602 0.12109679093504021 No Hit GTCCTACAGTGTGCATTTCTCATTT 581 0.11687248427451556 No Hit CTTTAGGACGTGAAATATGGCGAGG 563 0.11325164999406587 No Hit GTCCTAAAGTGTGTATTTCTCATTT 539 0.10842387095346626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.0115746002498375E-4 13 0.0 0.0 0.0 0.0 2.0115746002498375E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGTGT 50 8.509487E-5 15.238449 6 TTAGCAC 45 6.622745E-4 14.815159 3 TAGGACC 265 0.0 14.016499 4 AATGCCG 75 9.76619E-7 13.916588 18 GTCCTAG 70 7.0155056E-6 13.612631 1 GGGTTAG 50 0.0014632198 13.340378 1 TCCAACG 50 0.0015106731 13.284016 18 ATGCCGG 65 5.4996435E-5 13.138038 19 AGGACCT 305 0.0 12.802795 5 CTAAGAC 60 3.997179E-4 12.698708 3 GGACCTG 310 0.0 12.596298 6 TGTAGGA 580 0.0 12.486068 2 GTATAAT 55 0.0029945995 12.127616 1 TCTAAAT 270 0.0 11.9485855 18 GTATTAT 80 2.7698454E-5 11.911052 1 GCATTTC 280 0.0 11.859535 13 GTAGGAC 545 0.0 11.708441 3 GTCCTAC 475 0.0 11.635217 1 ACAGTCC 90 7.5320804E-6 11.597157 8 GGCGAGG 175 1.8189894E-12 11.386299 19 >>END_MODULE