FastQCFastQC Report
Thu 26 May 2016
SRR1926879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635144
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8340.13130880556220323No Hit
GTCCTACAGTGTGCATTTCTCATTT6430.10123688486390488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC250.00591034219.0650623
GCTTAAC456.546171E-414.8389521
TTGTGCG602.4655496E-514.308991
GGCGAGG1403.6379788E-1212.86876219
TAGGACG2400.012.6959734
CTAGGAC1001.3745557E-712.392293
TATACTG1551.8189894E-1212.282545
GTGCACC550.003108250212.06831311
CTTAGAC959.945925E-712.0410923
CTATACT1052.6297857E-711.7891194
TAGACAG1052.6406087E-711.7853915
TTAGGAC1700.011.775483
TCCTAGA657.7853457E-411.7323462
AGGACGT2150.011.5113125
GGACAAT752.0321114E-411.4227636
TAGGACC1950.011.2310524
TGGCGAG3050.011.1921618
GTCCTAC4200.011.1292151
GACTTAG600.005691365311.1292141
ACTATGT600.005820169611.0958188
Produced by FastQC (version 0.11.5)