##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926879_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635144 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.513318239643294 31.0 28.0 31.0 16.0 34.0 2 27.45283116899475 31.0 28.0 31.0 16.0 34.0 3 26.91540658496341 31.0 27.0 31.0 10.0 34.0 4 31.086663811671055 35.0 32.0 37.0 17.0 37.0 5 30.553948395954304 35.0 30.0 37.0 10.0 37.0 6 30.057632599851374 35.0 30.0 37.0 10.0 37.0 7 30.16922304233371 35.0 30.0 37.0 10.0 37.0 8 30.197086015139874 35.0 30.0 37.0 10.0 37.0 9 32.305596841031324 37.0 32.0 39.0 11.0 39.0 10 32.537249820513146 37.0 32.0 39.0 11.0 39.0 11 32.719284130842766 37.0 33.0 39.0 11.0 39.0 12 32.61813069162269 37.0 33.0 39.0 10.0 39.0 13 32.701631441059035 37.0 33.0 39.0 10.0 39.0 14 33.6347410980817 38.0 33.0 40.0 10.0 41.0 15 33.21408688423413 38.0 32.0 40.0 8.0 41.0 16 33.27639558903178 38.0 32.0 40.0 10.0 41.0 17 33.48541433123827 38.0 33.0 40.0 8.0 41.0 18 33.527870845036716 39.0 33.0 40.0 2.0 41.0 19 32.89502380562518 38.0 32.0 40.0 2.0 41.0 20 33.209831156399176 39.0 33.0 40.0 2.0 41.0 21 33.18048348091141 39.0 33.0 40.0 2.0 41.0 22 33.18436763946443 39.0 33.0 40.0 2.0 41.0 23 33.14718394568791 39.0 33.0 40.0 2.0 41.0 24 31.86586348922449 38.0 31.0 40.0 2.0 41.0 25 32.39433262378295 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.9348499787961799 1101 2 -1.0061086070887733 1101 3 -2.2190172416346385 1101 4 -1.147091461175794 1101 5 -1.2980556008606854 1101 6 -3.049501735304535 1101 7 -1.586960384702099 1101 8 -0.8857015571948992 1101 9 -1.2159039450375069 1101 10 -9.389198418991747 1101 11 -4.455069033287401 1101 12 -2.286829858165923 1101 13 -1.6912335298151149 1101 14 -1.6491227130837913 1101 15 -3.0317362858659713 1101 16 -1.347155400455467 1101 17 -1.1655385507831397 1101 18 -1.226787517035092 1101 19 -1.870029412274409 1101 20 -0.9824840360207077 1101 21 -0.9845096014539578 1101 22 -0.8355879628973 1101 23 -1.0465482255867968 1101 24 -1.110302317876723 1101 25 -0.8561136665268911 1102 1 -0.4593651985101985 1102 2 -0.46371619080288085 1102 3 -1.0044249571998947 1102 4 -0.36553243505562705 1102 5 -0.6684481990102213 1102 6 -1.5704592474098327 1102 7 -0.541435367084059 1102 8 0.07863952807641894 1102 9 -0.12961191748516399 1102 10 0.16466959346591636 1102 11 -0.002317208967689055 1102 12 0.04056790036462132 1102 13 -0.29134264800375576 1102 14 -0.4550446481130024 1102 15 0.17988480122398443 1102 16 0.09602130281125909 1102 17 -0.06237866990382912 1102 18 -0.5120724746381313 1102 19 0.03844543370981057 1102 20 0.22095318692139188 1102 21 0.2667145682248204 1102 22 0.06580329400783569 1102 23 -0.3676557752014702 1102 24 -4.05095111644 1102 25 -2.3122640677635644 1103 1 -0.7376570702069358 1103 2 -0.9426254308247515 1103 3 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0.07633087619957735 2213 19 0.6015174517143151 2213 20 -1.132854014879058 2213 21 0.07442274526908221 2213 22 -0.15676484802767732 2213 23 -0.09147422981512676 2213 24 -0.5097033044870116 2213 25 -0.039077021001872936 2214 1 0.6749001689573717 2214 2 0.546717749764813 2214 3 0.37265144135565365 2214 4 0.538298588366267 2214 5 1.0870179189263922 2214 6 1.1321855507503074 2214 7 1.4772638199078116 2214 8 -1.9750815475958738 2214 9 -0.6299629433279286 2214 10 -0.2754361177648974 2214 11 0.20867958338237003 2214 12 -1.1387054357930602 2214 13 -0.3284182396033444 2214 14 0.13024454472929392 2214 15 -1.3150918979226631 2214 16 -0.1592168500665494 2214 17 -0.05957248055161557 2214 18 0.052633289044813125 2214 19 0.8062256996018036 2214 20 -1.2301150210311604 2214 21 -0.5002587417067872 2214 22 -0.1017268155364377 2214 23 -0.2569373826577461 2214 24 -1.6149689501667233 2214 25 -0.49246540327568056 2215 1 0.31736140611014463 2215 2 -0.09388395683402706 2215 3 0.6459831630374495 2215 4 0.3925772604354698 2215 5 0.8965136997277661 2215 6 1.5021620591773193 2215 7 0.7667812528972675 2215 8 -0.7146377828479515 2215 9 -0.2866583919889436 2215 10 -0.4343756160157284 2215 11 -0.5724476267261878 2215 12 -0.6087830228003455 2215 13 -0.38423744032928653 2215 14 -0.3765138809674369 2215 15 -0.22962093655500126 2215 16 -0.02352630117657384 2215 17 -0.6618816969210926 2215 18 -1.2609625683100063 2215 19 -0.10259248683475164 2215 20 -4.184140033162343 2215 21 -2.3389569476933723 2215 22 -1.5007218106576872 2215 23 -1.061210186213188 2215 24 -0.8577099490052831 2215 25 -0.657030791657423 2216 1 -0.508990424159677 2216 2 -1.123452475238956 2216 3 0.00899235698266665 2216 4 -0.6956478106148474 2216 5 -0.026285103491815676 2216 6 -1.6420215266657792 2216 7 -9.933831960758024 2216 8 -10.529268014502655 2216 9 -5.86985797352472 2216 10 -3.6553131394435923 2216 11 -2.6394257561774808 2216 12 -2.14483552165877 2216 13 -1.9212049037136651 2216 14 -1.7542715533794677 2216 15 -2.9517375204148237 2216 16 -2.133717336228063 2216 17 -2.3811409334624223 2216 18 -1.9037234439498043 2216 19 -1.3836152365672447 2216 20 -2.3638419625959166 2216 21 -5.347407164763169 2216 22 -3.990359879997346 2216 23 -3.6467704443797153 2216 24 -4.1166602444519285 2216 25 -3.5586124705576943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45229.0 3 1.0 4 396.0 5 2011.0 6 2247.0 7 1826.0 8 1485.0 9 1402.0 10 1323.0 11 1224.0 12 1251.0 13 1334.0 14 1292.0 15 1332.0 16 1408.0 17 1630.0 18 1877.0 19 2249.0 20 2925.0 21 3767.0 22 4680.0 23 5516.0 24 6426.0 25 7463.0 26 8688.0 27 9131.0 28 10420.0 29 12119.0 30 14461.0 31 17668.0 32 22312.0 33 29290.0 34 38982.0 35 54264.0 36 76556.0 37 116762.0 38 124197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.562746010585855 18.41322679119371 12.57617947039196 25.44784772782848 2 14.944505212191517 19.579525717365883 41.398701916863416 24.077267153579186 3 20.04432122485456 23.87183411378828 28.65874705851757 27.425097602839582 4 12.06545578299889 16.177397398406775 38.41966446506 33.337482353534334 5 14.00135117471886 37.46801196214821 35.3949508899958 13.135685973137134 6 32.359175446055964 36.34541188327743 17.863813168296563 13.431599502370043 7 29.14743177701163 30.761504214434737 21.678793267643847 18.412270740909786 8 25.0146476123997 37.77109927369952 20.102367464770715 17.111885649130066 9 26.07683041092223 15.817868306471297 19.74143157463762 38.36386970796885 10 14.852703068791067 29.34913404776755 34.11045379803058 21.687709085410802 11 34.71585567682392 22.773432517593186 23.464562465455312 19.04614934012758 12 22.85968489925752 24.841465431039232 31.531805333564293 20.767044336138955 13 28.837571844736637 21.85670419651996 25.43532005353909 23.870403905204316 14 21.110853910680063 21.866708238654706 27.183828496652986 29.838609354012245 15 23.591843161955754 30.23116290055901 23.03298952838359 23.144004409101644 16 22.494771917005757 28.281724385542777 27.08225727207448 22.14124642537699 17 22.046221147214165 28.43721753480916 27.305152165080926 22.21140915289575 18 21.92445414434587 28.09176484667542 28.552195232561434 21.431585776417272 19 24.02091322115574 26.986874119101422 26.62105022795096 22.371162431791873 20 23.273882822760214 27.5378796229517 27.583388710596846 21.604848843691244 21 23.103268546089854 26.771308851387204 26.883268010701467 23.24215459182147 22 23.08134435414406 27.55318160559712 27.198558833209198 22.166915207049623 23 23.014600747044263 27.326797220944954 27.001618805862975 22.65698322614781 24 23.345201221326924 27.238928772201117 27.329788188905702 22.086081817566257 25 23.20007432438816 27.137849694671633 27.27468845169072 22.38738752924948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 210.0 1 210.0 2 241.5 3 273.0 4 273.0 5 273.0 6 409.5 7 546.0 8 546.0 9 546.0 10 835.0 11 1124.0 12 1124.0 13 1124.0 14 1958.5 15 2793.0 16 2793.0 17 2793.0 18 4806.5 19 6820.0 20 6820.0 21 6820.0 22 10549.5 23 14279.0 24 14279.0 25 14279.0 26 20482.5 27 26686.0 28 26686.0 29 26686.0 30 34900.5 31 43115.0 32 43115.0 33 43115.0 34 50094.5 35 57074.0 36 57074.0 37 57074.0 38 63296.0 39 69518.0 40 69518.0 41 69518.0 42 73991.0 43 78464.0 44 78464.0 45 78464.0 46 81196.0 47 83928.0 48 83928.0 49 83928.0 50 81615.0 51 79302.0 52 79302.0 53 79302.0 54 71909.5 55 64517.0 56 64517.0 57 64517.0 58 55543.0 59 46569.0 60 46569.0 61 46569.0 62 38225.0 63 29881.0 64 29881.0 65 29881.0 66 23375.5 67 16870.0 68 16870.0 69 16870.0 70 12400.0 71 7930.0 72 7930.0 73 7930.0 74 5662.0 75 3394.0 76 3394.0 77 3394.0 78 2320.5 79 1247.0 80 1247.0 81 1247.0 82 821.5 83 396.0 84 396.0 85 396.0 86 271.0 87 146.0 88 146.0 89 146.0 90 92.5 91 39.0 92 39.0 93 39.0 94 26.5 95 14.0 96 14.0 97 14.0 98 11.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2013716574509088 2 0.10548788936052297 3 0.10784955852531079 4 0.07179474260954996 5 0.02219969014900558 6 0.021097577872104596 7 0.3090637713652337 8 0.30465532225762976 9 0.017161462597458214 10 0.03715692819266182 11 0.015272127265627954 12 0.017161462597458214 13 0.014799793432670388 14 0.01590190570957137 15 0.014799793432670388 16 0.01763379643041578 17 0.01731890720844407 18 0.013540236544783545 19 0.02188480092703387 20 0.016689128764500648 21 0.01432745959971282 22 0.016216794931543082 23 0.01700401798647236 24 0.015272127265627954 25 0.014170014988726966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 635144.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.79518295194903 #Duplication Level Percentage of deduplicated Percentage of total 1 93.6498981617917 82.22009942545897 2 4.769758200833089 8.375235877574006 3 0.8141091528603305 2.1442458605468686 4 0.28625899500088553 1.0052864335097524 5 0.1378410617082044 0.605089061548135 6 0.08462310421860977 0.44577005501008166 7 0.05321193706047085 0.3270226224616145 8 0.032003175850241664 0.2247779743052291 9 0.02557404373621451 0.2020750063777889 >10 0.12824570031233126 1.9853840750319336 >50 0.010045545856424495 0.6247647581714499 >100 0.00753401430664193 1.341956392937437 >500 8.969064648508367E-4 0.49829245706672604 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 834 0.13130880556220323 No Hit GTCCTACAGTGTGCATTTCTCATTT 643 0.10123688486390488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.5744461098585516E-4 0.0 0.0 0.0 4 0.0 1.5744461098585516E-4 0.0 0.0 0.0 5 0.0 1.5744461098585516E-4 0.0 0.0 0.0 6 0.0 1.5744461098585516E-4 0.0 0.0 0.0 7 0.0 1.5744461098585516E-4 0.0 0.0 0.0 8 0.0 1.5744461098585516E-4 0.0 0.0 0.0 9 0.0 1.5744461098585516E-4 0.0 0.0 0.0 10 0.0 1.5744461098585516E-4 0.0 0.0 0.0 11 0.0 1.5744461098585516E-4 0.0 0.0 0.0 12 0.0 1.5744461098585516E-4 0.0 0.0 0.0 13 0.0 1.5744461098585516E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGAC 25 0.005910342 19.065062 3 GCTTAAC 45 6.546171E-4 14.838952 1 TTGTGCG 60 2.4655496E-5 14.30899 1 GGCGAGG 140 3.6379788E-12 12.868762 19 TAGGACG 240 0.0 12.695973 4 CTAGGAC 100 1.3745557E-7 12.39229 3 TATACTG 155 1.8189894E-12 12.28254 5 GTGCACC 55 0.0031082502 12.068313 11 CTTAGAC 95 9.945925E-7 12.041092 3 CTATACT 105 2.6297857E-7 11.789119 4 TAGACAG 105 2.6406087E-7 11.785391 5 TTAGGAC 170 0.0 11.77548 3 TCCTAGA 65 7.7853457E-4 11.732346 2 AGGACGT 215 0.0 11.511312 5 GGACAAT 75 2.0321114E-4 11.422763 6 TAGGACC 195 0.0 11.231052 4 TGGCGAG 305 0.0 11.19216 18 GTCCTAC 420 0.0 11.129215 1 GACTTAG 60 0.0056913653 11.129214 1 ACTATGT 60 0.0058201696 11.095818 8 >>END_MODULE