FastQCFastQC Report
Thu 26 May 2016
SRR1926879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635144
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14570.22939679820639097No Hit
GGTATCAACGCAGAGTACTTTTTTT9970.1569722771528976No Hit
TATCAACGCAGAGTACTTTTTTTTT7480.11776856901741968No Hit
GTCCTACAGTGGACATTTCTAAATT6910.10879422619122592No Hit
CTGTAGGACGTGGAATATGGCAAGA6860.10800700313629664No Hit
GTCCTACAGTGTGCATTTCTCATTT6670.10501555552756542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCC456.643125E-414.8102195
TTGTACC500.001473287813.3291974
TAGCACT1001.3966928E-712.3771114
GTGTAGA959.9508E-712.0406091
TCCTAGA1209.487849E-911.9142462
GACGTGA2250.011.8099247
CCCGTTT658.092899E-411.67830612
TGGCGAG3400.011.44216418
GGACGTG4000.011.4250266
GTAGCCC1604.3655746E-1111.3060153
GTGTAGC1701.0913936E-1111.21429351
TAGCCCT1803.6379788E-1211.1076644
AGGACGT4050.011.0488945
TCAGTAC700.001462290210.8809773
TGTAGGA4300.010.8613582
TCCCGTT700.001504259610.84414211
CGTTTCC700.001504259610.84414214
ACTGGTC700.001504259610.8441428
CTCCTAG1158.409297E-710.7754751
GGCGAGG1855.456968E-1210.77087119
Produced by FastQC (version 0.11.5)