FastQCFastQC Report
Thu 26 May 2016
SRR1926878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564910
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10040.1777274256076189No Hit
CTGTAGGACGTGGAATATGGCAAGA8030.14214653661645216No Hit
GTCCTACAGTGTGCATTTCTCATTT7990.14143845922359313No Hit
GTCCTAAAGTGTGTATTTCTCATTT7850.1389601883485865No Hit
GTATCAACGCAGAGTACTTTTTTTT6910.12232036961639908No Hit
CTTTAGGACGTGAAATATGGCGAGG6830.120904214830681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2050.014.3943324
GAACCAG500.001515269513.27918111
TATTTGG603.9931174E-412.7008032
TTAAGTA550.003019151512.1148784
AGTACGT550.003025184912.11164955
TGTAGGA4850.011.7842512
GTAGGAC4450.011.7732173
GTCCTAG657.7666057E-411.7352931
GTATTAT751.9999783E-411.4419121
GTCCTAA2250.011.4419111
CCAGTAC1001.8609553E-611.4307223
TTTAGGA3050.011.2433342
ACAGACC600.005831809211.0924958
TGGCGAG4800.011.06598318
AGGACCT3700.011.059485
CTGTAGG4700.010.9550221
AGGACGT4000.010.9437415
GGACGTG4000.010.9437416
CTGATAC700.001455783410.8864023
TTGTACT700.001464446910.8786664
Produced by FastQC (version 0.11.5)