##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926878_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 564910 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.482852135738437 31.0 28.0 31.0 16.0 34.0 2 27.430091519003028 31.0 28.0 31.0 16.0 34.0 3 26.884928572692996 31.0 27.0 31.0 10.0 34.0 4 31.035285266679647 35.0 32.0 37.0 16.0 37.0 5 30.471119293338763 35.0 30.0 37.0 10.0 37.0 6 30.00197730611956 35.0 30.0 37.0 10.0 37.0 7 30.115938822113257 35.0 30.0 37.0 10.0 37.0 8 30.174071976066983 35.0 30.0 37.0 10.0 37.0 9 32.25019206599281 37.0 32.0 39.0 11.0 39.0 10 32.47004832628206 37.0 32.0 39.0 10.0 39.0 11 32.668106424032146 37.0 33.0 39.0 10.0 39.0 12 32.56032996406507 37.0 33.0 39.0 10.0 39.0 13 32.64839708980192 37.0 33.0 39.0 10.0 39.0 14 33.57067497477474 38.0 33.0 40.0 10.0 41.0 15 33.185588854861834 38.0 32.0 40.0 8.0 41.0 16 33.225951036448286 38.0 32.0 40.0 8.0 41.0 17 33.44319980173833 38.0 33.0 40.0 2.0 41.0 18 33.46473243525517 38.0 33.0 40.0 2.0 41.0 19 32.84998318316192 38.0 32.0 40.0 2.0 41.0 20 33.176497141137524 39.0 32.0 40.0 2.0 41.0 21 33.14570816590253 39.0 33.0 40.0 2.0 41.0 22 33.14787842311165 39.0 33.0 40.0 2.0 41.0 23 33.107739285903946 39.0 33.0 40.0 2.0 41.0 24 31.814088969924413 38.0 31.0 40.0 2.0 41.0 25 32.354235187906035 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3336287102786031 1101 2 -0.6042324865118545 1101 3 -1.3973986184117635 1101 4 -0.6734663134939893 1101 5 -0.7806627586476615 1101 6 -2.6982422644151747 1101 7 -1.0784681971307393 1101 8 -0.4447040489662655 1101 9 -0.36186449865810033 1101 10 -8.919797453953745 1101 11 -4.419233301747173 1101 12 -1.829583166410071 1101 13 -1.0263594614789255 1101 14 -1.0118629748903345 1101 15 -2.4289831142549616 1101 16 -0.5592394609479996 1101 17 -0.42170191567435467 1101 18 -0.4409525273478607 1101 19 -0.984224275188204 1101 20 -0.3891922330099433 1101 21 -0.47475575929043146 1101 22 -0.24448021246111296 1101 23 -0.340353295936211 1101 24 -0.22561910117137884 1101 25 -0.06096348428336995 1102 1 -0.9214497541891227 1102 2 -0.9080960601103065 1102 3 -1.3456145808530806 1102 4 -1.0113812541181275 1102 5 -1.249519428075999 1102 6 -1.9386068045974447 1102 7 -1.01004787125391 1102 8 -0.15343091999636727 1102 9 -0.6514557717870701 1102 10 -0.6290600885850601 1102 11 -0.8233647571462335 1102 12 -0.5372108935572726 1102 13 -0.6966107732712494 1102 14 -1.0928488903832942 1102 15 -0.617654751924114 1102 16 -0.6316586821576138 1102 17 -0.7909921669861504 1102 18 -0.8537197187314547 1102 19 -0.4891041426281362 1102 20 -0.5095512498561163 1102 21 -0.7683017410634179 1102 22 -0.7763250067168386 1102 23 -1.2042721028956151 1102 24 -4.615961548009267 1102 25 -2.881374972822446 1103 1 -1.4607590339869638 1103 2 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10 -0.4751868680639788 2209 11 0.23047085929212585 2209 12 0.7158538391851437 2209 13 0.6067037580207213 2209 14 0.45167165756190997 2209 15 0.9653670047237526 2209 16 1.0374227038214343 2209 17 0.7264265813350974 2209 18 0.7647841759771126 2209 19 -1.788134494494912 2209 20 0.26224837410305923 2209 21 0.6258938001667715 2209 22 0.8317947891467128 2209 23 0.640208154960952 2209 24 0.3457198916898996 2209 25 0.8022753092081771 2210 1 -0.10174679031338485 2210 2 -0.15901635325512942 2210 3 0.43396645553060154 2210 4 0.15317286742023484 2210 5 0.25078668775512014 2210 6 0.10442711459316456 2210 7 0.6543596778737886 2210 8 0.3827387408117282 2210 9 -0.013247068239500948 2210 10 0.043834392250268195 2210 11 0.11723061220327224 2210 12 0.32730561205269026 2210 13 -0.07969527940004895 2210 14 -0.06032408332706751 2210 15 0.3099358830068155 2210 16 -0.20609801025930352 2210 17 0.1275369205033101 2210 18 -0.030882958889705492 2210 19 -0.9411516165239782 2210 20 0.10076589133332448 2210 21 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6 1.6219325156082505 2215 7 0.9441726229139036 2215 8 -0.1952721340939476 2215 9 0.13020483275325745 2215 10 -0.05887069939655021 2215 11 -0.025838104694209107 2215 12 -0.5142383612607659 2215 13 -0.2217907557119858 2215 14 -0.06086594795259259 2215 15 -0.04737505795697672 2215 16 0.12617902008746285 2215 17 -0.27931525741635 2215 18 -0.7974121807909071 2215 19 0.2016596852910908 2215 20 -3.489275318588085 2215 21 -2.294472567602991 2215 22 -1.4737688797884587 2215 23 -1.4147324064200077 2215 24 -1.4772007302493293 2215 25 -0.9223281899301838 2216 1 -0.3995626901310203 2216 2 -0.8237264991602302 2216 3 0.28904615329597405 2216 4 -0.2709624881690331 2216 5 0.3132326493957045 2216 6 -1.1601091274398208 2216 7 -8.943536060745505 2216 8 -10.250244372852482 2216 9 -5.113159625742444 2216 10 -3.2095403607829276 2216 11 -2.0671467371239878 2216 12 -1.9037056916989954 2216 13 -1.7251693023558516 2216 14 -1.7029712485590451 2216 15 -2.5983758145427096 2216 16 -2.0830726715575985 2216 17 -2.0918100692304726 2216 18 -1.8363644874287033 2216 19 -1.1816932939727671 2216 20 -2.2088013970785205 2216 21 -4.409037198761517 2216 22 -3.2071056126188253 2216 23 -3.2638481023721546 2216 24 -3.3895897623650875 2216 25 -2.7731776293689343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41111.0 3 9.0 4 356.0 5 1893.0 6 2067.0 7 1544.0 8 1336.0 9 1190.0 10 1183.0 11 1172.0 12 1166.0 13 1131.0 14 1133.0 15 1194.0 16 1293.0 17 1426.0 18 1580.0 19 1982.0 20 2621.0 21 3366.0 22 4291.0 23 5007.0 24 5797.0 25 6511.0 26 7465.0 27 7927.0 28 9094.0 29 10688.0 30 12877.0 31 15318.0 32 19791.0 33 25758.0 34 34715.0 35 47977.0 36 68161.0 37 103928.0 38 110852.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.831955727411405 18.97867962680478 12.509134768881477 25.680229876902338 2 14.327942411772836 20.416564767261757 42.10058091521615 23.154911905749252 3 19.436129999521512 25.088121301696503 29.23217061063765 26.243578088144343 4 11.641743985432543 17.090369960995208 39.48695057621522 31.780935477357026 5 13.300318170792384 38.07567755151906 36.0820197205692 12.54198455711936 6 30.689799871455964 37.56566634855264 18.69395010862502 13.050583671366372 7 28.16463111552936 31.252396506426187 22.7831427962792 17.799829581765252 8 24.148006141775614 38.48159686165917 20.999192338756203 16.37120465780902 9 26.30333054179953 15.593578646005776 20.396669281147588 37.706421531047106 10 14.212881552827259 29.745935445254908 35.25541925875555 20.785763743162285 11 33.80959715870562 22.957686880436533 24.737213909736496 18.495502051121345 12 22.736398917862353 25.189976062662 32.57779634849365 19.495828670981997 13 28.729002011274467 22.517421602787458 25.740595167275714 23.01298121866236 14 20.46430348280728 22.784935632424048 27.79801098790198 28.9527498968667 15 22.792884825457975 31.56911658737901 23.443002296302723 22.194996290860292 16 21.450867840075922 28.620043095434966 28.679888029773743 21.249201034715373 17 21.454636234709216 29.155047530015 28.138583324038024 21.251732911237763 18 21.147370321977544 28.248368512557935 29.962359823094708 20.64190134236981 19 23.51975664515946 27.35351610915555 27.29119007316227 21.835537172522717 20 22.948015870908115 27.979114989722152 28.469370953263677 20.60349818610605 21 22.40743462041688 27.341953234410436 27.21306192979319 23.037550215379497 22 22.544829393351367 28.820590359909914 27.436580218410505 21.19800002832821 23 21.97834300627118 28.33769471287486 27.864611919845046 21.81935036100891 24 23.034976054106213 27.859387587086037 28.030062940962967 21.07557341784478 25 22.373420298784396 27.85249158141857 28.203221545902956 21.570866573894076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 246.0 3 307.0 4 307.0 5 307.0 6 450.5 7 594.0 8 594.0 9 594.0 10 919.0 11 1244.0 12 1244.0 13 1244.0 14 2101.0 15 2958.0 16 2958.0 17 2958.0 18 5012.0 19 7066.0 20 7066.0 21 7066.0 22 11093.0 23 15120.0 24 15120.0 25 15120.0 26 21565.5 27 28011.0 28 28011.0 29 28011.0 30 36571.5 31 45132.0 32 45132.0 33 45132.0 34 51337.0 35 57542.0 36 57542.0 37 57542.0 38 62228.0 39 66914.0 40 66914.0 41 66914.0 42 69474.5 43 72035.0 44 72035.0 45 72035.0 46 72993.5 47 73952.0 48 73952.0 49 73952.0 50 70388.0 51 66824.0 52 66824.0 53 66824.0 54 58483.0 55 50142.0 56 50142.0 57 50142.0 58 42335.5 59 34529.0 60 34529.0 61 34529.0 62 28055.0 63 21581.0 64 21581.0 65 21581.0 66 16735.5 67 11890.0 68 11890.0 69 11890.0 70 8656.5 71 5423.0 72 5423.0 73 5423.0 74 3789.5 75 2156.0 76 2156.0 77 2156.0 78 1513.5 79 871.0 80 871.0 81 871.0 82 579.5 83 288.0 84 288.0 85 288.0 86 197.0 87 106.0 88 106.0 89 106.0 90 66.0 91 26.0 92 26.0 93 26.0 94 17.0 95 8.0 96 8.0 97 8.0 98 7.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20003186348267868 2 0.11116815067886919 3 0.11169920872351347 4 0.0644350427501726 5 0.021065302437556426 6 0.021773379830415465 7 0.2814607636614682 8 0.2752650864739516 9 0.018941070258979304 10 0.03735108247331433 11 0.017170876776831708 12 0.018055973517905506 13 0.016639818732187426 14 0.018941070258979304 15 0.01575472199111363 16 0.020711263741126903 17 0.019295108955408827 18 0.01557770264289887 19 0.024428670053636862 20 0.018232992866120266 21 0.016462799383972666 22 0.018055973517905506 23 0.018410012214335025 24 0.016816838080402186 25 0.01557770264289887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 564910.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.83812611379787 #Duplication Level Percentage of deduplicated Percentage of total 1 94.62005730198298 83.11248526186361 2 4.107605925371616 7.216088145971508 3 0.6168112248327781 1.6253862646580308 4 0.2278960970553307 0.8007186445595383 5 0.1154211291628615 0.506918784980219 6 0.06123023203098055 0.32270093058686256 7 0.04364225838574981 0.26834179371849115 8 0.02894454690660889 0.203394780919156 9 0.02110563985198733 0.16684918695281348 >10 0.13027735627013037 2.1089985692647963 >50 0.014310400133002991 0.8269626778477033 >100 0.011085457791750164 1.8095366919729485 >500 0.0014108764461900714 0.8538690407267556 >1k 2.0155377802715302E-4 0.17774922597753737 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1004 0.1777274256076189 No Hit CTGTAGGACGTGGAATATGGCAAGA 803 0.14214653661645216 No Hit GTCCTACAGTGTGCATTTCTCATTT 799 0.14143845922359313 No Hit GTCCTAAAGTGTGTATTTCTCATTT 785 0.1389601883485865 No Hit GTATCAACGCAGAGTACTTTTTTTT 691 0.12232036961639908 No Hit CTTTAGGACGTGAAATATGGCGAGG 683 0.120904214830681 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 205 0.0 14.394332 4 GAACCAG 50 0.0015152695 13.279181 11 TATTTGG 60 3.9931174E-4 12.700803 2 TTAAGTA 55 0.0030191515 12.114878 4 AGTACGT 55 0.0030251849 12.1116495 5 TGTAGGA 485 0.0 11.784251 2 GTAGGAC 445 0.0 11.773217 3 GTCCTAG 65 7.7666057E-4 11.735293 1 GTATTAT 75 1.9999783E-4 11.441912 1 GTCCTAA 225 0.0 11.441911 1 CCAGTAC 100 1.8609553E-6 11.430722 3 TTTAGGA 305 0.0 11.243334 2 ACAGACC 60 0.0058318092 11.092495 8 TGGCGAG 480 0.0 11.065983 18 AGGACCT 370 0.0 11.05948 5 CTGTAGG 470 0.0 10.955022 1 AGGACGT 400 0.0 10.943741 5 GGACGTG 400 0.0 10.943741 6 CTGATAC 70 0.0014557834 10.886402 3 TTGTACT 70 0.0014644469 10.878666 4 >>END_MODULE