FastQCFastQC Report
Thu 26 May 2016
SRR1926878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564910
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13090.23171832681312068No Hit
GTCCTACAGTGGACATTTCTAAATT10230.18109079322369936No Hit
CTGTAGGACGTGGAATATGGCAAGA9620.170292612982599No Hit
CTTTAGGACGTGAAATATGGCGAGG8430.14922731054504257No Hit
GTCCTACAGTGTGCATTTCTCATTT8310.14710307836646547No Hit
GGTATCAACGCAGAGTACTTTTTTT8080.14303163335752597No Hit
GTCCTAAAGTGTGTATTTCTCATTT8020.14196951726823742No Hit
TATCAACGCAGAGTACTTTTTTTTT7080.12532969853605No Hit
CTGTAGGACCTGGAATATGGCGAGA6460.11435449894673488No Hit
GTACATGGGAAGCAGTGGTATCAAC5660.10019295108955409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC500.001468454413.3345833
TAGGACC2250.012.6996024
CTAAACA853.818219E-612.3260864
GTCATGG701.0562895E-412.2536681
GTCCTAA2200.012.1298931
ATAATAC657.836865E-411.7227113
TCTAAAC657.836865E-411.7227113
TAGAAAT1552.3646862E-1111.6754424
GAATAAA1251.7362254E-811.4367571
TTGATGT752.0200205E-411.4296434
TATACTG752.0200205E-411.4296435
GTCATAT855.1373776E-511.2125061
GTATTAA600.00572882411.1190691
TGTCCAC1553.1650416E-1011.01786710
ATACACT2600.010.9900414
TTTAGAA1752.0008883E-1110.8911812
GACGTGA2550.010.7926957
AGGACCT3600.010.5830035
TCCTAAA2800.010.5508322
TTAGGAC2650.010.4232593
Produced by FastQC (version 0.11.5)