FastQCFastQC Report
Thu 26 May 2016
SRR1926877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647552
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12310.1901005633524412No Hit
GTCCTACAGTGTGCATTTCTCATTT10310.15921501284838901No Hit
GTCCTAAAGTGTGTATTTCTCATTT10250.15828844633326744No Hit
CTGTAGGACGTGGAATATGGCAAGA9900.1528834749950583No Hit
CTTTAGGACGTGAAATATGGCGAGG8450.13049145087962047No Hit
GTATCAACGCAGAGTACTTTTTTTT8330.12863831784937735No Hit
CTGTAGGACCTGGAATATGGCGAGA6660.10284888317849378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCG402.6659216E-416.6991351
GCACCGT402.7169424E-416.6577016
GTCTTAT905.1054303E-712.7231511
TTTGCGA603.9615858E-412.7132682
CTAGACT603.9954716E-412.7004444
TAGACAG1051.931403E-812.6915825
TAGGACC3600.012.4358524
GATATAC2650.012.24303151
CTGCGTG550.003109146612.0679059
GTATAAA1052.5580812E-711.8143551
GGCGAGG3050.011.81354719
GTATTGG657.71863E-411.7444461
AAAACGT657.881256E-411.7153065
CTAGAAC907.1433788E-611.653833
TACACTG4850.011.3831725
CCTACAC855.11638E-511.2175893
CAGGGGT855.169275E-511.2062744
GTCTAGG600.00567809611.1327571
GTAGCAC600.00571104711.124113
CACTAGA600.00571104711.124112
Produced by FastQC (version 0.11.5)