##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926877_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 647552 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.719500518877247 31.0 28.0 31.0 16.0 34.0 2 27.69775554704487 31.0 28.0 31.0 16.0 34.0 3 27.162728244218226 31.0 27.0 31.0 10.0 34.0 4 31.283978738387034 35.0 32.0 37.0 17.0 37.0 5 30.79809806779996 35.0 32.0 37.0 12.0 37.0 6 30.322100773374185 35.0 30.0 37.0 11.0 37.0 7 30.397568071753312 35.0 30.0 37.0 10.0 37.0 8 30.417005892963036 35.0 30.0 37.0 10.0 37.0 9 32.516514503854516 37.0 32.0 39.0 15.0 39.0 10 32.71138070764973 37.0 33.0 39.0 12.0 39.0 11 32.900892901265074 37.0 33.0 39.0 15.0 39.0 12 32.780016122257365 37.0 33.0 39.0 11.0 39.0 13 32.885119650622656 37.0 33.0 39.0 11.0 39.0 14 33.82846010822297 39.0 33.0 40.0 10.0 41.0 15 33.445485459082825 39.0 33.0 40.0 10.0 41.0 16 33.501893284245895 38.0 33.0 40.0 10.0 41.0 17 33.70811147212888 39.0 33.0 40.0 10.0 41.0 18 33.75220986113857 39.0 33.0 40.0 10.0 41.0 19 33.1484436771101 39.0 32.0 40.0 2.0 41.0 20 33.43669543140937 39.0 33.0 40.0 2.0 41.0 21 33.39821512403637 39.0 33.0 40.0 2.0 41.0 22 33.43230041757264 39.0 33.0 40.0 2.0 41.0 23 33.408555606345125 39.0 33.0 40.0 2.0 41.0 24 32.153717384858666 38.0 31.0 40.0 2.0 41.0 25 32.681840222870136 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.9747447721304106 1101 2 -0.9767456996084896 1101 3 -1.9277735682364323 1101 4 -1.3769541687932296 1101 5 -1.3710135397105176 1101 6 -3.2281185450518315 1101 7 -1.437673612527032 1101 8 -0.6540025712589035 1101 9 -0.9613372568508112 1101 10 -8.801896638681658 1101 11 -4.51572980373588 1101 12 -2.2700633926762777 1101 13 -1.6961495119445082 1101 14 -1.3675957874158016 1101 15 -2.584023914433388 1101 16 -1.3213482730808224 1101 17 -0.9838022919911111 1101 18 -0.8530178414228757 1101 19 -1.619927181671084 1101 20 -0.8121276815889544 1101 21 -0.8986319759469836 1101 22 -0.7127377867579341 1101 23 -0.7711007916160995 1101 24 -0.8031644341179245 1101 25 -0.624158264407221 1102 1 -0.8701268516111824 1102 2 -1.0409121637789873 1102 3 -1.3953348013989242 1102 4 -1.075578277296838 1102 5 -1.3249507931457671 1102 6 -1.6240924359233802 1102 7 -0.974851349668711 1102 8 -0.5177191020011804 1102 9 -0.46934434738955844 1102 10 -0.629312791269335 1102 11 -0.5615361173612001 1102 12 -0.6069994781658679 1102 13 -0.6810759582430848 1102 14 -0.8994511413212507 1102 15 -0.2338182641743245 1102 16 -0.5934437781137376 1102 17 -0.6340479189136587 1102 18 -0.9422643429049273 1102 19 -0.3759743518944063 1102 20 -0.5328587730190577 1102 21 -0.7422571538543323 1102 22 -0.6978814731886018 1102 23 -1.255183472762461 1102 24 -4.231024473243544 1102 25 -2.6326552650376662 1103 1 -0.8736512372333998 1103 2 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1110 8 1.736722121924796 1110 9 1.4689163446569182 1110 10 1.459439147863904 1110 11 1.1042966917887114 1110 12 1.1426049231095803 1110 13 1.0165280658275577 1110 14 0.9614784198258235 1110 15 1.6007162868535048 1110 16 1.200914315675547 1110 17 0.817110794154722 1110 18 1.0352919709750736 1110 19 -1.2730741236025942 1110 20 0.5479169424524528 1110 21 0.727130706990252 1110 22 1.1511578462676013 1110 23 1.048232199682836 1110 24 0.1620115384166425 1110 25 0.42698350954412234 1111 1 0.8223283986012966 1111 2 0.6157463470511075 1111 3 1.2195435049343004 1111 4 0.9998857039533213 1111 5 0.9565368314453693 1111 6 1.3418920594020385 1111 7 1.1301312655217508 1111 8 1.6569624340433258 1111 9 0.7901791800526894 1111 10 0.8429389922833508 1111 11 0.6703571527858614 1111 12 0.8083671481508716 1111 13 0.8308070097946256 1111 14 0.7697955169320281 1111 15 -0.2696654838925596 1111 16 0.27296773964452825 1111 17 0.6688382170226745 1111 18 0.8503119570925008 1111 19 1.487856487469955 1111 20 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2213 19 0.14747278448278678 2213 20 -1.2403032849363171 2213 21 -0.34667565948765144 2213 22 -0.7533431318432875 2213 23 -0.4900457135446885 2213 24 -0.5041278472500252 2213 25 -0.2987126820785093 2214 1 0.5789991078498282 2214 2 0.5288973150248744 2214 3 0.30804370706339057 2214 4 0.5654087691287266 2214 5 0.9556124277261944 2214 6 1.151674209157239 2214 7 1.27638109708084 2214 8 -2.322592881951824 2214 9 -0.48385756252468326 2214 10 -0.5057462702598841 2214 11 -0.14582575158461708 2214 12 -1.3348013376776358 2214 13 -0.26790924587621845 2214 14 -0.08361417628092482 2214 15 -1.4790056446974837 2214 16 -0.4945631411891469 2214 17 -0.10026477897966402 2214 18 -0.10781767503254969 2214 19 0.6354448628012221 2214 20 -1.0511067636689901 2214 21 -0.681681059140196 2214 22 -0.058765217726453045 2214 23 -0.49553927308955537 2214 24 -1.2933196731673107 2214 25 -0.6772596277533083 2215 1 0.8448069036021764 2215 2 0.5503690878828316 2215 3 1.0030037611195937 2215 4 1.0933924595244768 2215 5 1.4552050188466694 2215 6 2.1365735824762595 2215 7 1.7568999968781256 2215 8 0.03556920716141576 2215 9 0.721221468173411 2215 10 0.31722132251672264 2215 11 0.35994918647597984 2215 12 -0.1826672438477246 2215 13 0.37251432686779395 2215 14 0.5214045711520683 2215 15 0.5861565901555181 2215 16 0.6377387521028552 2215 17 0.4176678362541182 2215 18 -0.20874577233372094 2215 19 0.8841067068066835 2215 20 -3.269853865435092 2215 21 -1.3031518383179588 2215 22 -0.32320339094479067 2215 23 -0.2265964163252434 2215 24 -0.10802150286377454 2215 25 0.08539985791782101 2216 1 0.14817346145721189 2216 2 -0.481371951004121 2216 3 0.6497917052136586 2216 4 0.17048417822000417 2216 5 0.3054870839434898 2216 6 -1.250487869683596 2216 7 -8.975075568044385 2216 8 -10.269067435947004 2216 9 -5.197434444101173 2216 10 -3.076811732977209 2216 11 -2.1493613386721826 2216 12 -1.7940869186805486 2216 13 -1.5241817083744955 2216 14 -1.3790644809190553 2216 15 -2.696845026172568 2216 16 -1.554276270240507 2216 17 -1.7235073293402827 2216 18 -1.5136248646545738 2216 19 -0.7854603708087566 2216 20 -1.911292872819491 2216 21 -4.506408264014404 2216 22 -3.080747839967067 2216 23 -2.5820808393595023 2216 24 -2.7809670230995707 2216 25 -2.227012590977683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44343.0 3 3.0 4 398.0 5 1985.0 6 2377.0 7 1884.0 8 1449.0 9 1342.0 10 1316.0 11 1242.0 12 1316.0 13 1230.0 14 1315.0 15 1330.0 16 1367.0 17 1581.0 18 1747.0 19 2194.0 20 2927.0 21 3777.0 22 4479.0 23 5454.0 24 6424.0 25 7318.0 26 8295.0 27 9086.0 28 10239.0 29 11842.0 30 14012.0 31 17318.0 32 22252.0 33 28651.0 34 38799.0 35 53354.0 36 76873.0 37 118522.0 38 139511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68709377901578 18.968585577220676 12.740637573506655 25.60368307025689 2 14.025010591156617 20.35991378643899 42.72116963476744 22.893905987636952 3 19.60971069236847 24.960765425952182 29.31638296655127 26.11314091512808 4 11.528524231457473 17.12025639203009 39.79747625684943 31.553743119663007 5 13.172035952098879 38.245983655047674 36.179618391237796 12.40236200161565 6 30.57822645915839 37.90892578016397 18.626936122626763 12.885911638050873 7 27.73388483524655 31.302071230499916 22.917847903208315 18.04619603104522 8 23.863407531328903 38.887339870192235 21.082299363353314 16.166953235125547 9 26.02936497394345 15.77757252431893 20.41539112625456 37.77767137548306 10 14.175524889275437 29.914092877324755 35.35075780865004 20.559624424749778 11 33.73402980279741 22.899780062274502 25.020078584490683 18.346111550437406 12 22.710002069670676 25.17059337767165 32.80536137376708 19.314043178890593 13 28.627162675184024 22.59360394895762 25.85418140369628 22.925051972162073 14 20.125168353288604 23.13081513882196 28.061713064215194 28.68230344367424 15 22.601102146219713 32.00116145453647 23.417518194061753 21.980218205182066 16 21.217583368086128 28.777474012634567 29.04916361614383 20.955779003135476 17 21.015082595163996 29.301783190590562 28.433742383405285 21.24939183084016 18 20.82283730290501 28.38893719679922 30.480781259894325 20.30744424040144 19 23.481410543550453 27.303408968041893 27.331984368483653 21.883196119924005 20 22.878726332258868 28.33611088372438 28.48268522966146 20.302477554355296 21 22.392458005646613 27.390388405295706 27.360579982331174 22.85657360672651 22 22.31980131345644 28.98299796741689 27.715104749077923 20.982095970048746 23 21.813126983759236 28.45029307508746 28.125786746364557 21.610793194788748 24 22.902727581626664 27.754146974330464 28.529175547524172 20.813949896518704 25 22.344014011633114 27.979865999857907 28.244746409827915 21.431373578681065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 233.0 2 289.5 3 346.0 4 346.0 5 346.0 6 502.0 7 658.0 8 658.0 9 658.0 10 1021.5 11 1385.0 12 1385.0 13 1385.0 14 2337.0 15 3289.0 16 3289.0 17 3289.0 18 5718.0 19 8147.0 20 8147.0 21 8147.0 22 12933.0 23 17719.0 24 17719.0 25 17719.0 26 25517.5 27 33316.0 28 33316.0 29 33316.0 30 43824.0 31 54332.0 32 54332.0 33 54332.0 34 61029.0 35 67726.0 36 67726.0 37 67726.0 38 72673.5 39 77621.0 40 77621.0 41 77621.0 42 80646.0 43 83671.0 44 83671.0 45 83671.0 46 84132.0 47 84593.0 48 84593.0 49 84593.0 50 79831.0 51 75069.0 52 75069.0 53 75069.0 54 65540.5 55 56012.0 56 56012.0 57 56012.0 58 47193.5 59 38375.0 60 38375.0 61 38375.0 62 30849.0 63 23323.0 64 23323.0 65 23323.0 66 17938.5 67 12554.0 68 12554.0 69 12554.0 70 9066.0 71 5578.0 72 5578.0 73 5578.0 74 3921.5 75 2265.0 76 2265.0 77 2265.0 78 1558.5 79 852.0 80 852.0 81 852.0 82 586.0 83 320.0 84 320.0 85 320.0 86 217.5 87 115.0 88 115.0 89 115.0 90 75.5 91 36.0 92 36.0 93 36.0 94 22.0 95 8.0 96 8.0 97 8.0 98 8.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20878632140739276 2 0.12138021348092509 3 0.12369662976872899 4 0.06455080055346907 5 0.020847746590235226 6 0.019149041312512355 7 0.3153414706463728 8 0.3060758054951571 9 0.017604763787309744 10 0.03474624431705871 11 0.014825064241945048 12 0.016369341767147657 13 0.013898497726823483 14 0.016060486262107137 15 0.01358964222178296 16 0.018840185807471835 17 0.018068047044870528 18 0.013126358964222177 19 0.02192874085787705 20 0.015288347499505832 21 0.012971931211701917 22 0.015442775252026093 23 0.016523769519667917 24 0.014207353231864003 25 0.013898497726823483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 647552.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.75192339819255 #Duplication Level Percentage of deduplicated Percentage of total 1 94.41613235012028 81.90781081191244 2 4.211075552611466 7.306378075283025 3 0.6655365589723499 1.7320972974799786 4 0.23024837508923052 0.7989795759319693 5 0.1294111845938002 0.5613334586375355 6 0.06742419159402796 0.3509506982610103 7 0.0481371532752537 0.2923193443479304 8 0.031539656511741725 0.21889006925695384 9 0.029583351493281933 0.23097713783462995 >10 0.1438453906653088 2.3779725078188094 >50 0.012999033115509049 0.7903077819804009 >100 0.012286543483256532 2.167291933300454 >500 0.0012464609320833996 0.7569301830925714 >1k 5.341975423214571E-4 0.5077611248623025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1231 0.1901005633524412 No Hit GTCCTACAGTGTGCATTTCTCATTT 1031 0.15921501284838901 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1025 0.15828844633326744 No Hit CTGTAGGACGTGGAATATGGCAAGA 990 0.1528834749950583 No Hit CTTTAGGACGTGAAATATGGCGAGG 845 0.13049145087962047 No Hit GTATCAACGCAGAGTACTTTTTTTT 833 0.12863831784937735 No Hit CTGTAGGACCTGGAATATGGCGAGA 666 0.10284888317849378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.5442775252026093E-4 0.0 0.0 0.0 6 0.0 1.5442775252026093E-4 0.0 0.0 0.0 7 0.0 1.5442775252026093E-4 0.0 0.0 0.0 8 0.0 1.5442775252026093E-4 0.0 0.0 0.0 9 0.0 1.5442775252026093E-4 0.0 0.0 0.0 10 0.0 1.5442775252026093E-4 0.0 0.0 1.5442775252026093E-4 11 0.0 1.5442775252026093E-4 0.0 0.0 1.5442775252026093E-4 12 0.0 1.5442775252026093E-4 0.0 0.0 1.5442775252026093E-4 13 0.0 1.5442775252026093E-4 0.0 0.0 1.5442775252026093E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGCG 40 2.6659216E-4 16.699135 1 GCACCGT 40 2.7169424E-4 16.657701 6 GTCTTAT 90 5.1054303E-7 12.723151 1 TTTGCGA 60 3.9615858E-4 12.713268 2 CTAGACT 60 3.9954716E-4 12.700444 4 TAGACAG 105 1.931403E-8 12.691582 5 TAGGACC 360 0.0 12.435852 4 GATATAC 265 0.0 12.2430315 1 CTGCGTG 55 0.0031091466 12.067905 9 GTATAAA 105 2.5580812E-7 11.814355 1 GGCGAGG 305 0.0 11.813547 19 GTATTGG 65 7.71863E-4 11.744446 1 AAAACGT 65 7.881256E-4 11.715306 5 CTAGAAC 90 7.1433788E-6 11.65383 3 TACACTG 485 0.0 11.383172 5 CCTACAC 85 5.11638E-5 11.217589 3 CAGGGGT 85 5.169275E-5 11.206274 4 GTCTAGG 60 0.005678096 11.132757 1 GTAGCAC 60 0.005711047 11.12411 3 CACTAGA 60 0.005711047 11.12411 2 >>END_MODULE