FastQCFastQC Report
Thu 26 May 2016
SRR1926877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647552
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14980.23133277327535084No Hit
GTCCTACAGTGGACATTTCTAAATT12470.19257140739276538No Hit
CTGTAGGACGTGGAATATGGCAAGA11930.1842323087566713No Hit
GTCCTAAAGTGTGTATTTCTCATTT10330.15952386835342952No Hit
CTTTAGGACGTGAAATATGGCGAGG10120.15628088555050404No Hit
GTCCTACAGTGTGCATTTCTCATTT9820.15164805297489622No Hit
GGTATCAACGCAGAGTACTTTTTTT9370.14469880411148447No Hit
TATCAACGCAGAGTACTTTTTTTTT8020.12385105752124927No Hit
CTGTAGGACCTGGAATATGGCGAGA7650.11813723067799961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT400.00514472114.3036761
CGGTTTC759.804189E-713.91345613
CCAACGA655.512301E-513.13609619
TCCCGTT655.516297E-513.13508111
TTAGACA801.9369454E-613.0964544
CGTGATT751.4960564E-512.64957316
TCCAACG1155.4060365E-912.37458318
TAGGACA2800.012.2460354
GGCGAGG2900.012.10433419
GACAGTA802.8978184E-511.8598927
GTCTAGG657.763458E-411.7363491
GAATAAG657.763458E-411.7363491
GATATAC2450.011.676471
CCGGTTT658.108858E-411.67562812
TAGGACC3350.011.6570874
CTTTAGG4100.011.6290051
TGTAGAA3150.011.5016992
CTTAGAC752.0194556E-411.4305183
TTTAGGA4350.011.3973282
CCCGTTT752.097885E-411.38373712
Produced by FastQC (version 0.11.5)