FastQCFastQC Report
Thu 26 May 2016
SRR1926876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693640
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17680.25488726140360995No Hit
GTCCTAAAGTGTGTATTTCTCATTT14180.20442881033389076No Hit
CTGTAGGACGTGGAATATGGCAAGA13240.19087711204659477No Hit
GTCCTACAGTGTGCATTTCTCATTT13210.19044461103742574No Hit
CTTTAGGACGTGAAATATGGCGAGG10360.14935701516636873No Hit
CTGTAGGACCTGGAATATGGCGAGA9500.13695865290352344No Hit
GTATCAACGCAGAGTACTTTTTTTT7530.10855775330142438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3650.015.1353684
CCAACGA704.5203342E-714.90069719
CAAGAGT500.001468878113.3347834
GATATAC3150.013.3190151
GTCGAGG500.001519332613.275166519
CTCGAAC500.001519332613.275166518
TTATACT655.312147E-513.1882464
CCAATAC801.9293002E-613.1014063
GTAGAAC2850.013.0387193
GGCGAGG3050.012.74664719
GTCATAT603.9613372E-412.7136051
GTATTAT603.9613372E-412.7136051
CTACACT1352.5465852E-1112.6997934
GGACCTG6000.012.5355986
AATCCCG2200.012.49934419
AGGACCT6300.012.2409095
TGTAGGA9000.012.1759272
GATTAAT550.002981709512.1357151
ATACACT3200.011.9060554
ATCCCGT802.907642E-511.85624710
Produced by FastQC (version 0.11.5)