##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926876_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693640 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.62051640620495 31.0 28.0 31.0 16.0 34.0 2 27.572784153163024 31.0 28.0 31.0 16.0 34.0 3 27.026665128885302 31.0 27.0 31.0 10.0 34.0 4 31.170790323510754 35.0 32.0 37.0 17.0 37.0 5 30.649375756876765 35.0 31.0 37.0 10.0 37.0 6 30.181158814370566 35.0 30.0 37.0 10.0 37.0 7 30.286684735597717 35.0 30.0 37.0 10.0 37.0 8 30.343113142264 35.0 30.0 37.0 10.0 37.0 9 32.44249034081079 37.0 32.0 39.0 13.0 39.0 10 32.631049247448246 37.0 32.0 39.0 11.0 39.0 11 32.845474597774064 37.0 33.0 39.0 15.0 39.0 12 32.72010264690618 37.0 33.0 39.0 11.0 39.0 13 32.822513119197275 37.0 33.0 39.0 11.0 39.0 14 33.74969580762355 39.0 33.0 40.0 10.0 41.0 15 33.35762787613171 38.0 33.0 40.0 10.0 41.0 16 33.40289343175134 38.0 33.0 40.0 10.0 41.0 17 33.60237875555043 38.0 33.0 40.0 10.0 41.0 18 33.646292024681394 39.0 33.0 40.0 10.0 41.0 19 33.02723747188743 39.0 32.0 40.0 2.0 41.0 20 33.34360331007439 39.0 33.0 40.0 2.0 41.0 21 33.32283316994406 39.0 33.0 40.0 2.0 41.0 22 33.32634219479846 39.0 33.0 40.0 2.0 41.0 23 33.28862522345886 39.0 33.0 40.0 2.0 41.0 24 32.00977884781731 38.0 31.0 40.0 2.0 41.0 25 32.540822328585435 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5311183780325166 1101 2 -0.6057699064976632 1101 3 -1.633361321783589 1101 4 -0.7563444196295208 1101 5 -0.9570724896783993 1101 6 -2.9399549109692273 1101 7 -1.1701018878390705 1101 8 -0.5803818029829131 1101 9 -0.7423873414477811 1101 10 -9.127370847386732 1101 11 -4.242482511114691 1101 12 -1.7729736559023266 1101 13 -1.0583045000419133 1101 14 -0.8472342710992322 1101 15 -2.0879165946634366 1101 16 -0.520779970013102 1101 17 -0.4790351440912417 1101 18 -0.4948185984806841 1101 19 -1.080079250845806 1101 20 -0.5029658963082113 1101 21 -0.6227122841153232 1101 22 -0.4555439834118289 1101 23 -0.5898962348081582 1101 24 -0.6060895530211354 1101 25 -0.2563690824659943 1102 1 -0.6249108407234161 1102 2 -0.7549133941000079 1102 3 -0.9948008432980195 1102 4 -0.8016737655831072 1102 5 -0.9155814806390481 1102 6 -1.874886099634228 1102 7 -0.7120884159152538 1102 8 -0.12482753784151512 1102 9 -0.43901936049429224 1102 10 -0.11187329594759632 1102 11 -0.32511902080247523 1102 12 -0.4167554726764209 1102 13 -0.5868132974404432 1102 14 -0.7212224250972596 1102 15 -0.3078002638270547 1102 16 -0.34158983393892584 1102 17 -0.4678695129819346 1102 18 -0.6137151008131028 1102 19 -0.3475404900305179 1102 20 -0.30910375125275635 1102 21 -0.5225855009242579 1102 22 -0.5980821635407452 1102 23 -0.919173473838029 1102 24 -4.669140479215898 1102 25 -2.5728392835770393 1103 1 -0.9863045104564989 1103 2 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1107 11 0.010751772289388839 1107 12 0.3488761336854864 1107 13 0.20998206481833392 1107 14 0.19901690040966713 1107 15 -1.1874103190510397 1107 16 -0.45027810478715935 1107 17 -0.26329098325914657 1107 18 -0.11286902389920783 1107 19 1.031996938580292 1107 20 0.9807708036201177 1107 21 0.6880205751014685 1107 22 0.4428875729032953 1107 23 0.28963497786434544 1107 24 -3.534872837656451 1107 25 -1.410276868635755 1108 1 0.801339802980003 1108 2 0.24497785023214647 1108 3 0.8250467872227247 1108 4 0.9484689181349601 1108 5 1.082303825819551 1108 6 -2.6221472229222584 1108 7 -1.2085255058738547 1108 8 0.5026136550873233 1108 9 0.30104901614610213 1108 10 0.8583220738495569 1108 11 0.6080492270042868 1108 12 0.9557512803998804 1108 13 0.7413908583274207 1108 14 0.6886414351154571 1108 15 -0.09125657472326054 1108 16 0.738054637276143 1108 17 0.6435027976391225 1108 18 0.6423310636440576 1108 19 0.858366522943939 1108 20 -0.5795140296858605 1108 21 0.3729839604842127 1108 22 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0.8541004844518127 1110 10 0.7938314796979498 1110 11 0.33708008315378635 1110 12 0.4766169495706336 1110 13 0.625018059751234 1110 14 0.41338843952347304 1110 15 0.8212193674136969 1110 16 0.7026093857291968 1110 17 0.25953412977214896 1110 18 0.2853796346587316 1110 19 -1.7775474689022595 1110 20 -0.16015184761181445 1110 21 0.009306677315407796 1110 22 0.4034519226429012 1110 23 0.5593927115396085 1110 24 -0.5980201714223412 1110 25 -0.32033159054614657 1111 1 0.47121105510313654 1111 2 0.124432583217569 1111 3 0.8942365838636839 1111 4 0.6826602285512777 1111 5 0.7066797142004262 1111 6 0.9827009633696093 1111 7 0.7025620006045905 1111 8 1.2657847835427063 1111 9 0.4252196468516303 1111 10 0.5258508227527159 1111 11 0.4635734807643459 1111 12 0.37211904057681267 1111 13 0.43212398742937097 1111 14 0.5896867098271983 1111 15 -0.3750432709470317 1111 16 -0.029809194782025372 1111 17 0.23834210653652832 1111 18 0.44339532469076914 1111 19 0.8593261025762189 1111 20 0.44448828972587506 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0.6284056652590522 2215 3 1.1275944357316412 2215 4 1.0029501640783245 2215 5 1.4575767995788986 2215 6 1.929979174719378 2215 7 1.137333446226645 2215 8 -0.20908448949045066 2215 9 0.381641421160829 2215 10 0.3000551654632275 2215 11 0.1286433585880289 2215 12 -0.05106546987749283 2215 13 0.3509784321462419 2215 14 0.5058217560654512 2215 15 0.4333374417558815 2215 16 0.6683907746854558 2215 17 9.425515710645982E-4 2215 18 -0.6553981783601728 2215 19 0.4229817698273166 2215 20 -3.3748324697627226 2215 21 -1.8735844835893367 2215 22 -0.7595937187558803 2215 23 -0.4632725123140702 2215 24 -0.9877833510836531 2215 25 -0.5344043033455179 2216 1 -0.12868773600432348 2216 2 -0.6761389855433357 2216 3 0.3972732351721291 2216 4 -0.17875895699400246 2216 5 0.41913494503130266 2216 6 -0.9874047291691568 2216 7 -9.206728375860187 2216 8 -10.730219926208495 2216 9 -5.549475053755394 2216 10 -3.0656244045250816 2216 11 -2.1600540274434934 2216 12 -1.8572475348018962 2216 13 -1.330339664658144 2216 14 -1.6363536614463996 2216 15 -2.4528026168115 2216 16 -1.56815590599291 2216 17 -1.789067353418666 2216 18 -1.4243955605101988 2216 19 -0.8577427463879701 2216 20 -2.0226899586681277 2216 21 -4.53198740822085 2216 22 -3.195597278226792 2216 23 -3.1378806614323267 2216 24 -3.5747402478460586 2216 25 -2.8732706779898933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47967.0 3 6.0 4 407.0 5 2174.0 6 2300.0 7 1862.0 8 1556.0 9 1476.0 10 1341.0 11 1395.0 12 1298.0 13 1416.0 14 1387.0 15 1393.0 16 1555.0 17 1604.0 18 1906.0 19 2332.0 20 3157.0 21 4120.0 22 5096.0 23 5975.0 24 7056.0 25 8075.0 26 9074.0 27 10002.0 28 11408.0 29 13346.0 30 15677.0 31 19070.0 32 24531.0 33 31912.0 34 43021.0 35 59359.0 36 83834.0 37 127483.0 38 138069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97516721320948 18.76413908671973 12.51231527093596 25.748378429134828 2 14.094559331231887 19.896053436789174 42.999699793319245 23.009687438659693 3 19.966918816871406 24.181883000573006 29.02022253301984 26.830975649535755 4 11.612919979398665 16.678712964846447 39.27814541829508 32.4302216374598 5 13.183691479758549 38.02009580693259 36.24570642471715 12.550506288591706 6 31.434792640018454 37.235248312856896 18.3660667935629 12.963892253561745 7 28.317657538842422 30.877121438239683 22.402358888927452 18.402862133990443 8 23.97696924654098 39.264340360065184 20.660442425285243 16.098247968108595 9 26.154320889743808 15.527690579564904 20.268261276447255 38.04972725424403 10 14.395567310937215 29.627888329001983 35.183604126946584 20.79294023311422 11 34.327084180443926 22.440428299117272 24.830217408230887 18.40227011220791 12 23.129752940803268 24.679099893586034 32.648323475382035 19.542823690228662 13 29.14193846065115 22.326902557891398 25.5774720996626 22.953686881794848 14 20.22520028377139 22.864649121289197 27.68486956321123 29.22528103172818 15 22.614878182769825 32.20265911506058 23.079884476575938 22.102578225593657 16 21.301308431698825 28.386940484670557 29.200492572479963 21.111258511150652 17 21.120828256465344 29.254295210560848 28.526831097108168 21.09804543586564 18 21.00110304454714 28.058713691450322 30.674443250881357 20.265740013121185 19 23.695925221461625 27.02502375764463 27.00469096315438 22.27436005773937 20 22.908342693672505 28.184545995913652 28.548770130737143 20.3583411796767 21 22.351719812266143 27.216074170733997 27.09106245539158 23.341143561608284 22 22.513460434829003 29.115634291449055 27.452365777763678 20.918539495958267 23 21.904726199934537 28.448473505335848 27.96225615233657 21.684544142393047 24 23.296968543447417 27.456569886147353 28.418751658188278 20.827709912216953 25 22.424920551611173 27.823950444968652 28.341878961605232 21.409250041814943 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 244.0 3 307.0 4 307.0 5 307.0 6 431.0 7 555.0 8 555.0 9 555.0 10 852.5 11 1150.0 12 1150.0 13 1150.0 14 2052.0 15 2954.0 16 2954.0 17 2954.0 18 5275.5 19 7597.0 20 7597.0 21 7597.0 22 12478.0 23 17359.0 24 17359.0 25 17359.0 26 25607.0 27 33855.0 28 33855.0 29 33855.0 30 45329.5 31 56804.0 32 56804.0 33 56804.0 34 64280.5 35 71757.0 36 71757.0 37 71757.0 38 77234.5 39 82712.0 40 82712.0 41 82712.0 42 85757.0 43 88802.0 44 88802.0 45 88802.0 46 90412.0 47 92022.0 48 92022.0 49 92022.0 50 86862.0 51 81702.0 52 81702.0 53 81702.0 54 71640.0 55 61578.0 56 61578.0 57 61578.0 58 52305.0 59 43032.0 60 43032.0 61 43032.0 62 34672.0 63 26312.0 64 26312.0 65 26312.0 66 20223.5 67 14135.0 68 14135.0 69 14135.0 70 10364.0 71 6593.0 72 6593.0 73 6593.0 74 4636.5 75 2680.0 76 2680.0 77 2680.0 78 1871.0 79 1062.0 80 1062.0 81 1062.0 82 700.0 83 338.0 84 338.0 85 338.0 86 224.5 87 111.0 88 111.0 89 111.0 90 71.5 91 32.0 92 32.0 93 32.0 94 20.5 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20327547430944007 2 0.11302693039617093 3 0.11518943544201604 4 0.06934432847009976 5 0.023355054495127158 6 0.023066720489014474 7 0.30678738250389254 8 0.2945331872441036 9 0.019750879418718646 10 0.03546508275185976 11 0.015281702323972088 12 0.017588374372873535 13 0.014416700305634047 14 0.01787670837898622 15 0.014560867308690387 16 0.019895046421774983 17 0.01946254541260596 18 0.01513753532091575 19 0.02580589354708494 20 0.016002537339253792 21 0.01513753532091575 22 0.0181650423850989 23 0.01773254137592988 24 0.016146704342310133 25 0.015570036330084771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 693640.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.14963003976209 #Duplication Level Percentage of deduplicated Percentage of total 1 93.95708995705911 80.0041144945623 2 4.512518990549001 7.684786451852964 3 0.7302571563195339 1.865433800834911 4 0.2852365331346033 0.9715114108094326 5 0.13030637766788195 0.5547769925120832 6 0.08538358274853963 0.4362228289504538 7 0.04991028025327451 0.2974889329123018 8 0.04083696728317533 0.2781802124886598 9 0.02476320752628958 0.1897720163515269 >10 0.1503506679774703 2.5155806550661723 >50 0.017265878887949865 1.0213375381913101 >100 0.013710657351560565 2.3555254678593327 >500 0.0015234063696402784 0.8355247582546422 >1k 8.463368720223769E-4 0.989744439353912 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1768 0.25488726140360995 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1418 0.20442881033389076 No Hit CTGTAGGACGTGGAATATGGCAAGA 1324 0.19087711204659477 No Hit GTCCTACAGTGTGCATTTCTCATTT 1321 0.19044461103742574 No Hit CTTTAGGACGTGAAATATGGCGAGG 1036 0.14935701516636873 No Hit CTGTAGGACCTGGAATATGGCGAGA 950 0.13695865290352344 No Hit GTATCAACGCAGAGTACTTTTTTTT 753 0.10855775330142438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.4416700305634048E-4 0.0 0.0 0.0 0.0 9 1.4416700305634048E-4 0.0 0.0 0.0 1.4416700305634048E-4 10 1.4416700305634048E-4 0.0 0.0 0.0 1.4416700305634048E-4 11 1.4416700305634048E-4 0.0 0.0 0.0 1.4416700305634048E-4 12 1.4416700305634048E-4 0.0 0.0 0.0 1.4416700305634048E-4 13 1.4416700305634048E-4 0.0 0.0 0.0 1.4416700305634048E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 365 0.0 15.135368 4 CCAACGA 70 4.5203342E-7 14.900697 19 CAAGAGT 50 0.0014688781 13.334783 4 GATATAC 315 0.0 13.319015 1 GTCGAGG 50 0.0015193326 13.2751665 19 CTCGAAC 50 0.0015193326 13.2751665 18 TTATACT 65 5.312147E-5 13.188246 4 CCAATAC 80 1.9293002E-6 13.101406 3 GTAGAAC 285 0.0 13.038719 3 GGCGAGG 305 0.0 12.746647 19 GTCATAT 60 3.9613372E-4 12.713605 1 GTATTAT 60 3.9613372E-4 12.713605 1 CTACACT 135 2.5465852E-11 12.699793 4 GGACCTG 600 0.0 12.535598 6 AATCCCG 220 0.0 12.499344 19 AGGACCT 630 0.0 12.240909 5 TGTAGGA 900 0.0 12.175927 2 GATTAAT 55 0.0029817095 12.135715 1 ATACACT 320 0.0 11.906055 4 ATCCCGT 80 2.907642E-5 11.856247 10 >>END_MODULE