FastQCFastQC Report
Thu 26 May 2016
SRR1926876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693640
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18130.26137477654114527No Hit
GTCCTACAGTGGACATTTCTAAATT17730.25560809641889165No Hit
GTCCTACAGTGTGCATTTCTCATTT13370.1927512830863272No Hit
GTATCAACGCAGAGTACTTTTTTTT13270.1913096130557638No Hit
GTCCTAAAGTGTGTATTTCTCATTT13260.19116544605270747No Hit
CTTTAGGACGTGAAATATGGCGAGG13250.19102127904965113No Hit
CTGTAGGACCTGGAATATGGCGAGA10910.15728620033446744No Hit
GGTATCAACGCAGAGTACTTTTTTT7470.10769275128308634No Hit
GTACATGGGAAGCAGTGGTATCAAC7300.10524191223112854No Hit
GATATACACTGTTCTACAAATCCCG7220.10408857620667783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG653.2554908E-614.6622951
TCCAACG1152.5465852E-1114.02658418
TAGGACC4650.013.9279844
CTAATCT500.00146953313.3339974
GACGTGA5000.012.7137687
TGTAGGA10650.012.7073232
CCCTTAT751.4279347E-512.7073221
TAACCTG603.9998392E-412.6990455
GGACGTG9000.012.3815696
CCAACGA1155.3969416E-912.37639719
GTAGGAC10300.012.2992363
TTAGGAC6200.012.2902883
AGGACGT9400.012.2599825
ACCTAAT550.00299277712.1297171
CTGTAGG10850.012.1217321
TAATGGA951.0054991E-612.0306754
TAGGACG9650.011.9423664
GTCCTAA4250.011.8850841
GTCCTAC9950.011.5898441
AGGACCT7750.011.4291415