FastQCFastQC Report
Thu 26 May 2016
SRR1926875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640254
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7810.12198283806114447No Hit
GTATCAACGCAGAGTACTTTTTTTT7220.11276774530108365No Hit
GATATACACTGTTCTACAAATCCCG6570.10261552446372846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTT655.3624317E-513.1746847
GTATTGG701.050421E-412.2615061
GCTATAG550.002977710712.1376521
CTAAGCA550.003011174712.1195624
GTCTATG802.7493877E-511.9209091
GTATTAA751.997267E-411.4440731
GTCCAAG751.997267E-411.4440731
TCAATAC752.0104284E-411.43598653
GGACCTG2500.011.4180596
GGGTATA600.005703013411.1261821
ATCTTAG600.005703013411.1261821
AATACTC600.005800477711.1008915
GTGTAGC2000.010.9672361
GTAGGAC4200.010.8914163
CCAAAAC700.001450658510.8914163
GAAGTGT1053.3902597E-610.8743426
TGGATAG700.001469777810.8743425
CTAGACT1158.5199827E-710.764584
GGATAGG803.6322259E-410.7288181
TGTAGAA2400.010.7212382