##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926875_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 640254 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.51095502722357 31.0 28.0 31.0 16.0 34.0 2 27.45843368413161 31.0 28.0 31.0 16.0 34.0 3 26.91276587104493 31.0 27.0 31.0 10.0 34.0 4 31.04234881781293 35.0 32.0 37.0 16.0 37.0 5 30.516694936696997 35.0 30.0 37.0 10.0 37.0 6 30.010947217822928 35.0 30.0 37.0 10.0 37.0 7 30.127882059307712 35.0 30.0 37.0 10.0 37.0 8 30.16823167055575 35.0 30.0 37.0 10.0 37.0 9 32.25518466108763 37.0 32.0 39.0 11.0 39.0 10 32.466050973519884 37.0 32.0 39.0 10.0 39.0 11 32.65861829836284 37.0 33.0 39.0 10.0 39.0 12 32.56188950010465 37.0 33.0 39.0 10.0 39.0 13 32.64560471312948 37.0 33.0 39.0 10.0 39.0 14 33.56491017627379 38.0 33.0 40.0 10.0 41.0 15 33.16061438116748 38.0 32.0 40.0 8.0 41.0 16 33.212100197734024 38.0 32.0 40.0 8.0 41.0 17 33.412503475183286 38.0 33.0 40.0 2.0 41.0 18 33.44177310879745 38.0 33.0 40.0 2.0 41.0 19 32.81408472262571 38.0 32.0 40.0 2.0 41.0 20 33.138685584158786 39.0 32.0 40.0 2.0 41.0 21 33.13216317274082 39.0 33.0 40.0 2.0 41.0 22 33.139460276702685 39.0 33.0 40.0 2.0 41.0 23 33.11337687855133 39.0 33.0 40.0 2.0 41.0 24 31.793494456887423 38.0 31.0 40.0 2.0 41.0 25 32.338228265657065 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.1084661162842515 1101 2 -1.0786308600215762 1101 3 -2.02315837784883 1101 4 -1.4652007403947245 1101 5 -1.6519360366103903 1101 6 -3.7819492793369207 1101 7 -1.857076890979176 1101 8 -1.341948705982123 1101 9 -1.2508010902561537 1101 10 -9.542119103482591 1101 11 -5.007878181177951 1101 12 -2.821207327465796 1101 13 -1.9817049088477212 1101 14 -1.9768924475932437 1101 15 -3.2565269212904298 1101 16 -1.6671136716623742 1101 17 -1.7303894363555052 1101 18 -1.6396550573963964 1101 19 -2.2329868984918235 1101 20 -1.540802056679638 1101 21 -1.6569386616928092 1101 22 -1.3015218280221532 1101 23 -1.514359049817049 1101 24 -1.520540055409409 1101 25 -1.303746603358828 1102 1 -0.8992773717614213 1102 2 -1.0507501650343514 1102 3 -1.449701924516848 1102 4 -1.1654716382275403 1102 5 -1.024220714070676 1102 6 -1.9067726987823512 1102 7 -0.9524610541353766 1102 8 -0.7321652973641477 1102 9 -0.7271318943638185 1102 10 -0.6441488282283991 1102 11 -0.6611955693929445 1102 12 -0.783651709877951 1102 13 -0.9609011818349131 1102 14 -1.155306479297387 1102 15 -0.7845567052488747 1102 16 -0.838192737456076 1102 17 -0.9310526179353005 1102 18 -1.0295903887334177 1102 19 -0.774856579927814 1102 20 -0.7252155977647519 1102 21 -0.8415700207075076 1102 22 -0.7889486581157072 1102 23 -1.2676125729691918 1102 24 -4.715065212322909 1102 25 -2.903324290343889 1103 1 -0.1348471226275052 1103 2 -0.5609028279327894 1103 3 -0.35886977911414064 1103 4 -0.3203629425753647 1103 5 -0.5964683064391885 1103 6 0.050991187574126684 1103 7 -0.2331997950394218 1103 8 0.10120774876623884 1103 9 -0.04423996013650111 1103 10 0.19417726701600913 1103 11 -0.2669790648926167 1103 12 -0.3341228763153197 1103 13 -0.024592565746466732 1103 14 -0.1950987019432091 1103 15 0.03653369074641688 1103 16 0.24759622804972992 1103 17 -0.2347871403043058 1103 18 -0.40096340171225364 1103 19 -1.6668011671081082 1103 20 -0.35211974005121505 1103 21 -0.09995529147281701 1103 22 -0.17853637712315162 1103 23 -0.18162884209349528 1103 24 1.2920944869925108 1103 25 0.6876218135199252 1104 1 -0.6516105760567044 1104 2 -0.6063431229230645 1104 3 -1.6971877864349167 1104 4 -0.5403433014718928 1104 5 -0.8496111944288423 1104 6 -2.0841109523926136 1104 7 -0.7456768786885135 1104 8 0.06341108194039435 1104 9 -0.08748256928892317 1104 10 0.08036475442888502 1104 11 0.016441435353414136 1104 12 -0.046893538403210755 1104 13 -0.2007541626138547 1104 14 -0.4111342303324932 1104 15 0.16488913243247794 1104 16 -0.2839904450670758 1104 17 -0.2709051779379905 1104 18 -0.2998023758071753 1104 19 -1.3405439600065847 1104 20 -0.5077920948866392 1104 21 -0.19213975174733378 1104 22 -0.14863871433141895 1104 23 0.03904705270825559 1104 24 1.2511990519919038 1104 25 0.4846166831640346 1105 1 -0.11842856670513058 1105 2 -0.22095557684552958 1105 3 -0.02047271258716421 1105 4 0.061686996310612585 1105 5 -0.08114513305810078 1105 6 -0.1906335520314144 1105 7 -0.1904910429430693 1105 8 0.41450745923602383 1105 9 -0.36787931025689957 1105 10 -0.10073747790908527 1105 11 -0.06700322624988075 1105 12 -0.21138724217105676 1105 13 -0.2820176147798108 1105 14 -0.4015290745966169 1105 15 0.4447580949354659 1105 16 -0.2146288096135578 1105 17 -0.46402055525835806 1105 18 -0.37811975208848025 1105 19 -1.1254639273502889 1105 20 -0.325320937155098 1105 21 -0.2637093978883627 1105 22 -0.5646703975323959 1105 23 -0.49137236624063974 1105 24 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1108 22 0.647785486610239 1108 23 0.7194527524158048 1108 24 -0.22515587302832074 1108 25 0.4310377729116155 1109 1 0.3431369761857148 1109 2 0.4860572898058919 1109 3 -1.0739373202686373 1109 4 0.251673716417816 1109 5 0.6397444191567523 1109 6 1.0158978683554274 1109 7 0.9705186301147961 1109 8 1.3366234958098921 1109 9 0.715694746208726 1109 10 0.7255286254654507 1109 11 0.45945324900177553 1109 12 0.4830508498573991 1109 13 0.49934860935749725 1109 14 0.46977131176401343 1109 15 -1.0727715641604547 1109 16 0.18487128868171965 1109 17 0.17454670077877665 1109 18 0.23097998351727966 1109 19 -1.2206669171315951 1109 20 0.05437366543107203 1109 21 0.1656092754062115 1109 22 0.7542345930593441 1109 23 0.67935951232257 1109 24 -0.29625144412389304 1109 25 0.34874229061613704 1110 1 0.5476896374547948 1110 2 0.6682939586097234 1110 3 0.861982481322805 1110 4 0.7149388095336526 1110 5 0.7702964541864006 1110 6 0.7121806900113832 1110 7 0.89638182164963 1110 8 1.0798494241104493 1110 9 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0.661554156173203 2210 21 1.0669066082103953 2210 22 1.1579952159980493 2210 23 1.3871927749503499 2210 24 1.620304602569142 2210 25 1.2927092825283282 2211 1 0.3166370083960466 2211 2 0.3440020399546846 2211 3 0.19718328193954804 2211 4 0.3089629142267434 2211 5 0.6449855687674884 2211 6 -1.626296318593333 2211 7 0.3566387659943189 2211 8 0.39961632588645557 2211 9 0.635639094449509 2211 10 0.48308771699951336 2211 11 0.715531146080636 2211 12 0.7279195518964556 2211 13 0.5919662900406308 2211 14 0.7196225192668066 2211 15 1.176032567223075 2211 16 0.46403966602141367 2211 17 0.40871291954557165 2211 18 0.31725655583812795 2211 19 0.5989206175666268 2211 20 0.2783739192677075 2211 21 0.07260699376100632 2211 22 -2.769554418698867 2211 23 -1.1474239063761544 2211 24 1.008270790138475 2211 25 0.7146574149528178 2212 1 -0.978372733616677 2212 2 -0.35893089274326684 2212 3 -3.05579448749166 2212 4 -1.78297163822754 2212 5 -0.5805201942785914 2212 6 0.24573729721924664 2212 7 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2215 5 1.1117447470731108 2215 6 1.4416896338418503 2215 7 0.760630236066099 2215 8 -0.4679383545534854 2215 9 0.020326183473748927 2215 10 -0.3105078571359172 2215 11 -0.40419550682255334 2215 12 -0.4570186226548216 2215 13 -0.11051480966622762 2215 14 0.05780827480097628 2215 15 -0.21525217515252137 2215 16 0.19372598360511972 2215 17 -0.3312528431887074 2215 18 -1.038451920456609 2215 19 0.0822639306465831 2215 20 -3.7993927971140202 2215 21 -2.4872026073675038 2215 22 -1.3336317390817953 2215 23 -0.8789800488795905 2215 24 -0.7762199489015593 2215 25 -0.8592043429030198 2216 1 -0.051236256215684506 2216 2 -0.5051486873155788 2216 3 0.4441220851147101 2216 4 -0.27500383138649553 2216 5 0.1240418119048492 2216 6 -1.4011920839457943 2216 7 -8.905851478649677 2216 8 -10.481684956301375 2216 9 -5.410397471432791 2216 10 -3.0446378277995976 2216 11 -1.9983846877093967 2216 12 -1.7961488237084744 2216 13 -1.270205779915198 2216 14 -1.3136705984384633 2216 15 -2.543955309992093 2216 16 -1.4751517868343136 2216 17 -1.8383488543703024 2216 18 -1.4061715972962716 2216 19 -0.8675974513860716 2216 20 -1.6859142157018816 2216 21 -4.1352377648774485 2216 22 -2.9398107981691446 2216 23 -3.058536394587424 2216 24 -3.420570786753096 2216 25 -2.7621679259278835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46842.0 3 6.0 4 388.0 5 2083.0 6 2243.0 7 1800.0 8 1471.0 9 1410.0 10 1326.0 11 1361.0 12 1321.0 13 1309.0 14 1312.0 15 1351.0 16 1472.0 17 1606.0 18 1748.0 19 2150.0 20 2937.0 21 3729.0 22 4787.0 23 5635.0 24 6525.0 25 7539.0 26 8303.0 27 9174.0 28 10480.0 29 12459.0 30 14281.0 31 17679.0 32 22403.0 33 29078.0 34 39173.0 35 54253.0 36 76638.0 37 116965.0 38 127017.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34178815584204 18.65485390786486 12.698820028071472 25.304537908221626 2 14.50154080434521 20.036710386631665 41.95052853263206 23.51122027639106 3 20.071265406570866 24.122667809081257 28.948014403179585 26.858052381168296 4 11.739264316719265 16.64032862374738 39.10597584402877 32.514431215504594 5 13.67609632026347 37.70069976393317 35.680773911725694 12.942430004077659 6 31.520261719738656 37.120864150082646 18.232891821883097 13.125982308295603 7 28.54187352588337 31.0782154927504 21.938770715939096 18.44114026542713 8 24.485797470059342 38.30540646784012 20.554162562077828 16.654633500022708 9 25.965502642526957 15.947646252892095 19.974629241673657 38.1122218629073 10 14.333194264097168 29.900510805992162 34.57073702007441 21.195557909836257 11 34.51285544227783 22.95092467326337 23.9151218993998 18.621097985059006 12 22.462268144956564 25.14806072090847 32.15218033178724 20.237490802347725 13 28.72711366618759 22.037586702493282 25.71455352121477 23.520746110104355 14 20.806040145785847 22.21234555557812 27.63260466445978 29.349009634176255 15 23.260863791942587 30.621388434554802 23.386150647289384 22.73159712621323 16 22.038725441183825 28.708506224066387 27.66069464580313 21.59207368894666 17 21.65014325841819 28.620126167392073 27.70230369534855 22.027426878841187 18 21.332498094154992 28.712023694964824 29.01836484746991 20.937113363410276 19 23.631156786956346 27.192039570919246 27.000028122099906 22.176775520024496 20 22.822349458292 28.06477062356957 28.14444505198366 20.968434866154773 21 22.953606008817015 27.255259120860583 27.087010994710415 22.70412387561199 22 22.419481834201942 28.10652643945359 27.645976278854683 21.828015447489783 23 22.857763293975022 27.490150007342578 27.240658520048367 22.41142817863403 24 22.95677902422793 27.678795992682726 27.708321291489685 21.65610369159966 25 22.83038469049014 27.420425698105838 27.70099589923843 22.04819371216559 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 202.0 2 245.0 3 288.0 4 288.0 5 288.0 6 415.5 7 543.0 8 543.0 9 543.0 10 874.5 11 1206.0 12 1206.0 13 1206.0 14 1990.0 15 2774.0 16 2774.0 17 2774.0 18 4993.5 19 7213.0 20 7213.0 21 7213.0 22 11322.0 23 15431.0 24 15431.0 25 15431.0 26 22243.0 27 29055.0 28 29055.0 29 29055.0 30 38565.0 31 48075.0 32 48075.0 33 48075.0 34 54891.5 35 61708.0 36 61708.0 37 61708.0 38 67306.0 39 72904.0 40 72904.0 41 72904.0 42 77322.0 43 81740.0 44 81740.0 45 81740.0 46 83196.5 47 84653.0 48 84653.0 49 84653.0 50 81444.0 51 78235.0 52 78235.0 53 78235.0 54 69779.0 55 61323.0 56 61323.0 57 61323.0 58 52179.5 59 43036.0 60 43036.0 61 43036.0 62 34814.0 63 26592.0 64 26592.0 65 26592.0 66 20532.5 67 14473.0 68 14473.0 69 14473.0 70 10493.5 71 6514.0 72 6514.0 73 6514.0 74 4617.0 75 2720.0 76 2720.0 77 2720.0 78 1852.5 79 985.0 80 985.0 81 985.0 82 688.0 83 391.0 84 391.0 85 391.0 86 265.0 87 139.0 88 139.0 89 139.0 90 88.5 91 38.0 92 38.0 93 38.0 94 24.5 95 11.0 96 11.0 97 11.0 98 8.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18289616308527554 2 0.10199077241219892 3 0.10511453266984665 4 0.0773130663767817 5 0.028270030331712104 6 0.028113842318829713 7 0.2711423903638243 8 0.2719233304282363 9 0.022959637893710933 10 0.04451358367148038 11 0.021241569752004672 12 0.022647261867946158 13 0.0209291937262399 14 0.02139775776488706 15 0.01921112558453364 16 0.024677706035417198 17 0.023740577958122868 18 0.0184301855201217 19 0.02936334642188881 20 0.02327201391947571 21 0.019992065648945574 22 0.023428201932358096 23 0.023740577958122868 24 0.019523501610298415 25 0.020148253661827962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 640254.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.06571062068299 #Duplication Level Percentage of deduplicated Percentage of total 1 94.34522782748972 83.08579532298118 2 4.32137743098016 7.611303486388985 3 0.647073394492632 1.7095493492919351 4 0.2371157279093555 0.8352706031071164 5 0.12086390807271215 0.5321982976408149 6 0.07716184152507234 0.4077187444023612 7 0.04679173586800585 0.28845232292738654 8 0.033769982745414484 0.2379182022498504 9 0.0233350112004508 0.18495129093383672 >10 0.12619404568892922 2.0638225078354866 >50 0.009924398502572256 0.5982185195599993 >100 0.00974695641220404 1.6732023815801065 >500 0.001417739112800409 0.7715989711008588 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 781 0.12198283806114447 No Hit GTATCAACGCAGAGTACTTTTTTTT 722 0.11276774530108365 No Hit GATATACACTGTTCTACAAATCCCG 657 0.10261552446372846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGTT 65 5.3624317E-5 13.174684 7 GTATTGG 70 1.050421E-4 12.261506 1 GCTATAG 55 0.0029777107 12.137652 1 CTAAGCA 55 0.0030111747 12.119562 4 GTCTATG 80 2.7493877E-5 11.920909 1 GTATTAA 75 1.997267E-4 11.444073 1 GTCCAAG 75 1.997267E-4 11.444073 1 TCAATAC 75 2.0104284E-4 11.4359865 3 GGACCTG 250 0.0 11.418059 6 GGGTATA 60 0.0057030134 11.126182 1 ATCTTAG 60 0.0057030134 11.126182 1 AATACTC 60 0.0058004777 11.100891 5 GTGTAGC 200 0.0 10.967236 1 GTAGGAC 420 0.0 10.891416 3 CCAAAAC 70 0.0014506585 10.891416 3 GAAGTGT 105 3.3902597E-6 10.874342 6 TGGATAG 70 0.0014697778 10.874342 5 CTAGACT 115 8.5199827E-7 10.76458 4 GGATAGG 80 3.6322259E-4 10.728818 1 TGTAGAA 240 0.0 10.721238 2 >>END_MODULE