FastQCFastQC Report
Thu 26 May 2016
SRR1926875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640254
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12620.19710927225757277No Hit
GGTATCAACGCAGAGTACTTTTTTT8010.12510659831879223No Hit
GATATACACTGTTCTACAAATCCCG7580.11839051376484958No Hit
CTGTAGGACGTGGAATATGGCAAGA7260.11339249735261318No Hit
GTCCTACAGTGGACATTTCTAAATT7150.11167442921090692No Hit
TATCAACGCAGAGTACTTTTTTTTT7090.11073730113361259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGC250.00607530618.97445910
TAGGACC2100.014.9704754
ATTTAGG400.00514514614.3034621
CCAACGA801.3081626E-714.22862219
TCCAACG954.984031E-913.97899618
ACTGTTC2800.013.5542438
TCTACAC1051.9046638E-812.7042153
AGTAGGC603.9853735E-412.7042153
AATCCCG1800.012.64766319
TTAGGAC2250.012.2807413
GTCTAAG701.0515409E-412.2601111
TTAAGTT701.0608294E-412.2485714
CACTGTT3500.012.1988197
CTACACT1251.3424142E-912.1941344
TTTAGGA2700.012.00595952
AGGACCT3500.011.7041915
GGACCTG3400.011.488046
TACACTG6100.011.4007665
CATCTAA1354.432877E-911.30151
GATATAC3650.011.2337691
Produced by FastQC (version 0.11.5)