FastQCFastQC Report
Thu 26 May 2016
SRR1926874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551630
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14240.25814404582781936No Hit
CTGTAGGACGTGGAATATGGCAAGA10420.1888947301633341No Hit
GTCCTAAAGTGTGTATTTCTCATTT9880.1791055598861556No Hit
GTCCTACAGTGTGCATTTCTCATTT9730.17638634592027264No Hit
CTTTAGGACGTGAAATATGGCGAGG7950.14411834019179523No Hit
CTGTAGGACCTGGAATATGGCGAGA6980.12653408987908563No Hit
GTATCAACGCAGAGTACTTTTTTTT6030.10931240142849374No Hit
GTACATGGGAAGCAGTGGTATCAAC5740.10405525442778675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG250.005917710719.060413
TGTACTG508.557823E-515.2289015
TCCAACG902.3355824E-914.7508418
ATTCAAC400.00512608914.3109551
GTAAGAC602.4867273E-514.2953073
GGGCTAT400.005207314614.2770966
TAGGACC2950.013.5548424
ACTGTCC500.001485251813.3143848
TGCCGGT500.001516062613.27816510
GCCGGTT500.001518136113.27575811
GTCCTAC7800.012.3539011
CACCTTT1251.4424586E-912.137835514
ATCCCGT550.003102067212.07105810
TGTAGGA7200.011.91492752
CCAACGA802.9123768E-511.85335419
GTGTAGG1052.56141E-711.8122161
GGCGAGG2650.011.80862619
GACGTGG2750.011.7677887
GTCCAGA657.727151E-411.7423221
TTTACAC657.7984337E-411.7294843
Produced by FastQC (version 0.11.5)