FastQCFastQC Report
Thu 26 May 2016
SRR1926874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551630
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14450.2619509453800555No Hit
CTGTAGGACGTGGAATATGGCAAGA12890.233671120134873No Hit
GTCCTACAGTGTGCATTTCTCATTT11220.2033972046480431No Hit
CTTTAGGACGTGAAATATGGCGAGG10660.1932454725087468No Hit
GTATCAACGCAGAGTACTTTTTTTT10650.19306419157768792No Hit
GTCCTAAAGTGTGTATTTCTCATTT9890.17928684081721444No Hit
CTGTAGGACCTGGAATATGGCGAGA8730.15825825281438644No Hit
GGTATCAACGCAGAGTACTTTTTTT7190.13034098943132172No Hit
GTACATGGGAAGCAGTGGTATCAAC6370.11547595308449504No Hit
TATCAACGCAGAGTACTTTTTTTTT5930.10749959211790512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2600.015.0105964
ATTACAC500.001474111513.3276933
GGCGAGG2350.013.32699519
GTGTAAT655.2894953E-513.1932321
TCCAACG655.49171E-513.14060218
CTAGACA801.9487015E-613.0885074
ATTAGAC604.013512E-412.693043
GTCCTAA3350.012.5149731
AGGACCT5550.012.177355
CCTACAC1103.6850906E-812.1160843
TGATACT550.003018804112.1149824
AATCCCG1103.8469807E-812.07873619
ACTGTTA550.003091530212.0765478
GTAGGAC6600.011.6833673
CTAAAGT3450.011.58824354
TGTAGGA7200.011.3811992
AAAGTGT3600.011.3698156
GATATAC2350.011.3530411
GTGTAGG1354.480171E-911.2929721
GTCCTAG1201.2170312E-711.116521