##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926874_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 551630 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.801544513532622 31.0 30.0 33.0 26.0 34.0 2 30.17403150662582 31.0 30.0 33.0 26.0 34.0 3 30.248605043235504 31.0 30.0 34.0 26.0 34.0 4 33.851594365788664 35.0 35.0 37.0 29.0 37.0 5 33.827023548392944 37.0 35.0 37.0 30.0 37.0 6 33.776495114478905 37.0 35.0 37.0 29.0 37.0 7 33.780711708935335 37.0 35.0 37.0 29.0 37.0 8 33.816891757156064 37.0 35.0 37.0 29.0 37.0 9 35.36100103330131 39.0 35.0 39.0 30.0 39.0 10 35.24609792795896 39.0 35.0 39.0 29.0 39.0 11 35.28723419683484 39.0 35.0 39.0 30.0 39.0 12 35.18070264488878 39.0 35.0 39.0 29.0 39.0 13 35.20017584250313 39.0 35.0 39.0 29.0 39.0 14 36.27142468683719 40.0 36.0 41.0 29.0 41.0 15 36.26719358990628 40.0 36.0 41.0 29.0 41.0 16 36.24967097511013 40.0 36.0 41.0 29.0 41.0 17 36.22708699671882 40.0 36.0 41.0 29.0 41.0 18 36.203844968547756 40.0 36.0 41.0 29.0 41.0 19 36.28061381723256 40.0 37.0 41.0 29.0 41.0 20 36.2467378496456 40.0 37.0 41.0 29.0 41.0 21 36.19360440875224 40.0 37.0 41.0 29.0 41.0 22 36.10765368090931 40.0 36.0 41.0 29.0 41.0 23 36.03368924822798 40.0 36.0 41.0 29.0 41.0 24 35.961454235628956 40.0 36.0 41.0 28.0 41.0 25 35.84935010786215 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030858384392583815 1101 2 0.018849626685884857 1101 3 0.26834711955419976 1101 4 0.1829312726557646 1101 5 0.24504690116121708 1101 6 0.06406111824036032 1101 7 -0.015870633670324708 1101 8 0.271212166556964 1101 9 -0.03813898138797356 1101 10 -0.04303615310967501 1101 11 -0.030787852749618594 1101 12 0.038347065667849733 1101 13 -0.002240584567822168 1101 14 0.11589423304443613 1101 15 -0.01923198297308204 1101 16 -0.12044768468320655 1101 17 -0.05484640881002889 1101 18 -0.06591603086423703 1101 19 0.03464742573686408 1101 20 -0.0306329965974399 1101 21 -0.12567554158299288 1101 22 -0.3855953307709328 1101 23 -0.19942818588511102 1101 24 -0.022406455248102475 1101 25 -0.0549079850573122 1102 1 -0.826684084626212 1102 2 -0.7003766689243349 1102 3 -0.4984430411316225 1102 4 -0.7954888654852184 1102 5 -0.9071551695175728 1102 6 -0.9649240768371072 1102 7 -0.6816728422029001 1102 8 -0.7754728806148847 1102 9 -0.7787871813908396 1102 10 -0.7658566990681166 1102 11 -0.7882987302751445 1102 12 -0.5861191098946961 1102 13 -0.8449508474952054 1102 14 -0.717006871221848 1102 15 -0.759941929985537 1102 16 -0.9524426874833551 1102 17 -0.6949575534272725 1102 18 -0.791202149274902 1102 19 -0.8966439483494923 1102 20 -0.8661116795193706 1102 21 -0.9169548534628333 1102 22 -0.7456292198273502 1102 23 -0.7631683314931763 1102 24 -0.7455228124617292 1102 25 -0.6606849203952692 1103 1 -0.9545466297549439 1103 2 -0.7532574412295681 1103 3 -0.6352522750631557 1103 4 -0.7719384750283496 1103 5 -1.0235543578592043 1103 6 -0.9271213519840273 1103 7 -0.8638128444867874 1103 8 -0.9473046459718262 1103 9 -0.7539015953541792 1103 10 -0.7454993585668888 1103 11 -0.6830774128236143 1103 12 -1.043309401617357 1103 13 -0.9779740992234878 1103 14 -1.0418986061855264 1103 15 -0.9887478962015734 1103 16 -0.9277020404405505 1103 17 -0.8566084524882385 1103 18 -0.9794910785710229 1103 19 -0.9313502492428469 1103 20 -0.9483711466709224 1103 21 -1.0839553190244473 1103 22 -0.990347730732914 1103 23 -1.1158488244614375 1103 24 -1.078895674611573 1103 25 -1.0066630829201983 1104 1 -0.27333323237388285 1104 2 -0.1021084571464499 1104 3 0.21804771835659764 1104 4 0.0056857636737319694 1104 5 -0.11662974554537442 1104 6 -0.059292175172814154 1104 7 -0.16437362768230201 1104 8 -0.18268004901189983 1104 9 -0.19861802330413525 1104 10 -0.14722776987613884 1104 11 0.09496065024438849 1104 12 0.007209341116954704 1104 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2214 15 0.0482484407947652 2214 16 -0.0465054592439742 2214 17 -0.22303091891070892 2214 18 0.05347595439601349 2214 19 0.08220286431026835 2214 20 0.10144570314592727 2214 21 -0.12620196127108585 2214 22 -0.08876042914560855 2214 23 0.11150160288899258 2214 24 0.04674535102945754 2214 25 0.2388802626231481 2215 1 0.4747386749297213 2215 2 0.33600828062401433 2215 3 0.16324741481033556 2215 4 0.15231263772115966 2215 5 0.3327985544042491 2215 6 0.19298537834635 2215 7 -0.05844746084664365 2215 8 0.015913179865805205 2215 9 -0.1841855464049047 2215 10 0.22060908038499605 2215 11 -0.016449755780620023 2215 12 0.0018169310489071222 2215 13 0.04602072029848614 2215 14 -0.18494689619867444 2215 15 0.027858500596515512 2215 16 -0.1258592258181821 2215 17 0.0406597035658578 2215 18 0.01652993981421247 2215 19 0.21195160332418084 2215 20 0.17392402354757053 2215 21 0.07496598686171296 2215 22 0.00726878126059205 2215 23 0.2410034109850443 2215 24 -0.2916073505820336 2215 25 -0.27677351916481996 2216 1 -0.2121285674678859 2216 2 -0.2272058605928322 2216 3 -1.6424143269850333 2216 4 -1.472970460994837 2216 5 -0.5465920915956275 2216 6 -0.2808451391139215 2216 7 -0.2608461847515713 2216 8 -0.22904801177632805 2216 9 -0.4248764235342435 2216 10 -0.27838641328800406 2216 11 -0.3223928523573889 2216 12 -0.2212729955198185 2216 13 -0.30306034243200486 2216 14 -0.6889079529765354 2216 15 -0.5269615021007681 2216 16 -0.41565465066390317 2216 17 -0.5242041997783105 2216 18 -0.2875552851068477 2216 19 -0.4255518264138587 2216 20 -0.4619354571784626 2216 21 -0.4181617482090445 2216 22 -0.46231002365590257 2216 23 -0.47464456535325183 2216 24 -0.33856104563065514 2216 25 -0.5247168389652188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12807.0 3 10.0 4 231.0 5 1344.0 6 1818.0 7 1259.0 8 965.0 9 929.0 10 853.0 11 784.0 12 726.0 13 703.0 14 676.0 15 660.0 16 706.0 17 732.0 18 807.0 19 1017.0 20 1334.0 21 1645.0 22 2226.0 23 2546.0 24 3204.0 25 3619.0 26 4354.0 27 4868.0 28 5664.0 29 6949.0 30 8860.0 31 11547.0 32 14933.0 33 19757.0 34 27046.0 35 37866.0 36 55232.0 37 95301.0 38 217652.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40461938865517 19.63548718469932 10.65025717718546 28.309636249460056 2 13.688496480847428 20.75292990544829 41.08907747216159 24.46949614154269 3 18.657917925250114 24.74226991039732 29.222766550750073 27.37704561360249 4 11.994815365371716 17.46097927958958 38.273480412595404 32.2707249424433 5 12.671725833294053 38.78456724546143 35.59787239442645 12.945834526818054 6 31.45718313702306 38.26076674700987 17.737596070762653 12.54445404520442 7 28.03772038612447 31.730706468527664 21.579890196761024 18.651682948586846 8 24.551158747396148 39.15937105116691 19.65637333729765 16.633096864139294 9 26.052289501308053 15.781849694424954 19.879691723639052 38.28616908062794 10 14.51444773381145 30.115716023487654 34.20194376814011 21.167892474560784 11 34.07498454515625 22.722699313097912 24.64653276698809 18.555783374757752 12 23.342361156431508 25.040063231018006 31.977890897488887 19.639684715061602 13 29.159249201451708 22.337960864788826 25.624789259389512 22.878000674369954 14 20.30763374000689 23.08250095172489 27.434874825517102 29.174990482751117 15 22.350714543401057 32.259508965557245 23.039787096341442 22.349989394700252 16 21.243521437493087 28.93186881715362 28.64435569687488 21.18025404847841 17 20.829178978663236 29.681126842267464 28.126099015644545 21.36359516342476 18 20.769181962383865 28.305461137548154 30.518740086109226 20.40661681395876 19 23.245291227815745 27.267008683356597 27.390460997407683 22.097239091419972 20 22.96809795042293 28.220207169350246 28.352724490868813 20.458970389358008 21 22.216903905880304 27.21823650673643 27.212616013329633 23.352243574053627 22 22.372423544767326 29.171908706923116 27.36254373402462 21.093124014284935 23 21.55289152673264 28.638632124126907 27.92547164851739 21.88300470062306 24 22.60573210304008 27.466961550314522 28.872795170675996 21.054511175969402 25 22.143763596809283 28.00543872371283 28.309825960841188 21.54097171863669 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 164.0 3 164.0 4 164.0 5 164.0 6 274.5 7 385.0 8 385.0 9 385.0 10 661.5 11 938.0 12 938.0 13 938.0 14 1542.5 15 2147.0 16 2147.0 17 2147.0 18 3996.0 19 5845.0 20 5845.0 21 5845.0 22 9787.5 23 13730.0 24 13730.0 25 13730.0 26 19996.5 27 26263.0 28 26263.0 29 26263.0 30 35333.5 31 44404.0 32 44404.0 33 44404.0 34 50468.0 35 56532.0 36 56532.0 37 56532.0 38 60554.5 39 64577.0 40 64577.0 41 64577.0 42 67953.5 43 71330.0 44 71330.0 45 71330.0 46 73153.0 47 74976.0 48 74976.0 49 74976.0 50 71089.5 51 67203.0 52 67203.0 53 67203.0 54 58075.0 55 48947.0 56 48947.0 57 48947.0 58 41581.0 59 34215.0 60 34215.0 61 34215.0 62 27503.5 63 20792.0 64 20792.0 65 20792.0 66 15977.0 67 11162.0 68 11162.0 69 11162.0 70 8072.0 71 4982.0 72 4982.0 73 4982.0 74 3420.5 75 1859.0 76 1859.0 77 1859.0 78 1272.5 79 686.0 80 686.0 81 686.0 82 469.0 83 252.0 84 252.0 85 252.0 86 157.5 87 63.0 88 63.0 89 63.0 90 38.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11747004332614253 2 0.09118430832260754 3 0.0152275982089444 4 0.0 5 0.004350742345412686 6 0.34534017366713193 7 0.01776553124376847 8 0.007795080035531063 9 0.008520203759766509 10 0.0038068995522361 11 0.004169461414353824 12 3.6256186211772385E-4 13 7.251237242354477E-4 14 0.0 15 0.003444337690118377 16 5.438427931765857E-4 17 0.0 18 9.064046552943096E-4 19 0.0 20 7.251237242354477E-4 21 0.013958631691532369 22 0.0 23 1.8128093105886192E-4 24 0.0 25 0.005438427931765857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 551630.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.97212102479794 #Duplication Level Percentage of deduplicated Percentage of total 1 92.55115442329559 76.79165585794445 2 5.652037685399982 9.379231097394523 3 0.8771736274273326 2.18342869123985 4 0.31117685736984063 1.0327601547922682 5 0.15226342182487312 0.6316809531651616 6 0.09639585566047638 0.479890116129 7 0.0663763099152517 0.38551682536274323 8 0.0455357179869825 0.3022556083013584 9 0.03146170241327459 0.23493997622523566 >10 0.17859501034577516 2.7982003627964507 >50 0.019680043794593958 1.1481061854827335 >100 0.015525342941993767 2.6621588318032816 >500 0.0015306676139912043 0.8839390600770457 >1k 0.0010933340099937175 1.0862362792859754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1445 0.2619509453800555 No Hit CTGTAGGACGTGGAATATGGCAAGA 1289 0.233671120134873 No Hit GTCCTACAGTGTGCATTTCTCATTT 1122 0.2033972046480431 No Hit CTTTAGGACGTGAAATATGGCGAGG 1066 0.1932454725087468 No Hit GTATCAACGCAGAGTACTTTTTTTT 1065 0.19306419157768792 No Hit GTCCTAAAGTGTGTATTTCTCATTT 989 0.17928684081721444 No Hit CTGTAGGACCTGGAATATGGCGAGA 873 0.15825825281438644 No Hit GGTATCAACGCAGAGTACTTTTTTT 719 0.13034098943132172 No Hit GTACATGGGAAGCAGTGGTATCAAC 637 0.11547595308449504 No Hit TATCAACGCAGAGTACTTTTTTTTT 593 0.10749959211790512 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.8128093105886192E-4 0.0 12 0.0 0.0 0.0 1.8128093105886192E-4 0.0 13 0.0 0.0 0.0 1.8128093105886192E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 260 0.0 15.010596 4 ATTACAC 50 0.0014741115 13.327693 3 GGCGAGG 235 0.0 13.326995 19 GTGTAAT 65 5.2894953E-5 13.193232 1 TCCAACG 65 5.49171E-5 13.140602 18 CTAGACA 80 1.9487015E-6 13.088507 4 ATTAGAC 60 4.013512E-4 12.69304 3 GTCCTAA 335 0.0 12.514973 1 AGGACCT 555 0.0 12.17735 5 CCTACAC 110 3.6850906E-8 12.116084 3 TGATACT 55 0.0030188041 12.114982 4 AATCCCG 110 3.8469807E-8 12.078736 19 ACTGTTA 55 0.0030915302 12.076547 8 GTAGGAC 660 0.0 11.683367 3 CTAAAGT 345 0.0 11.5882435 4 TGTAGGA 720 0.0 11.381199 2 AAAGTGT 360 0.0 11.369815 6 GATATAC 235 0.0 11.353041 1 GTGTAGG 135 4.480171E-9 11.292972 1 GTCCTAG 120 1.2170312E-7 11.11652 1 >>END_MODULE