FastQCFastQC Report
Thu 26 May 2016
SRR1926873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609674
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16200.26571577597207685No Hit
GTCCTACAGTGTGCATTTCTCATTT12950.21240859869372813No Hit
CTGTAGGACGTGGAATATGGCAAGA12140.1991228098951243No Hit
GTCCTAAAGTGTGTATTTCTCATTT11980.19649845655219017No Hit
CTTTAGGACGTGAAATATGGCGAGG8900.14597965470070892No Hit
CTGTAGGACCTGGAATATGGCGAGA7730.12678907088050337No Hit
GTATCAACGCAGAGTACTTTTTTTT6920.1135032820818995No Hit
GATATACACTGTTCTACAAATCCCG6480.10628631038883075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG350.002108696116.3536341
GTTTATA653.2217267E-614.6763391
CCAACGA759.847772E-713.90764119
GTATTAT706.9401867E-613.6280281
TAGGACC3100.013.5166774
CTATACT852.6114503E-713.4443954
GTCTAAA500.001451459513.3554691
GGTCTAC801.9026375E-613.1169781
CTGTAGG7650.013.0935961
TGTAGGA8000.012.8689272
TCTAAAG603.9694598E-412.7100522
TCCAACG1052.0361767E-812.64331118
GTAGGAC7850.012.5076633
GACGTGG3700.012.3471877
ATTATAC550.002987242812.1323223
TAGCACA1103.669993E-812.1203254
AGACTTA550.00302249712.1133386
AGGACGT6300.012.0858715
CTAGGAC802.7612685E-511.9156733
GGACGTG6400.011.7483176
Produced by FastQC (version 0.11.5)