##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926873_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609674 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.88337209721917 31.0 28.0 31.0 16.0 34.0 2 27.84996407916362 31.0 28.0 31.0 16.0 34.0 3 27.319324753884864 31.0 27.0 31.0 12.0 34.0 4 31.47200143027257 35.0 32.0 37.0 18.0 37.0 5 30.98086190324665 35.0 32.0 37.0 16.0 37.0 6 30.51706485761243 35.0 30.0 37.0 11.0 37.0 7 30.613744394545282 35.0 31.0 37.0 11.0 37.0 8 30.652630750204207 35.0 31.0 37.0 10.0 37.0 9 32.77036252160991 37.0 32.0 39.0 17.0 39.0 10 32.961602430151196 37.0 33.0 39.0 17.0 39.0 11 33.143962183068325 37.0 33.0 39.0 17.0 39.0 12 33.03746428419122 37.0 33.0 39.0 17.0 39.0 13 33.15088719545199 38.0 33.0 39.0 17.0 39.0 14 34.09953844185581 39.0 34.0 40.0 17.0 41.0 15 33.72212198650426 39.0 33.0 40.0 10.0 41.0 16 33.776997214905016 39.0 33.0 40.0 11.0 41.0 17 33.99528272486607 39.0 33.0 40.0 16.0 41.0 18 34.01913481631167 39.0 34.0 40.0 11.0 41.0 19 33.41599772993436 39.0 33.0 40.0 10.0 41.0 20 33.741399502028955 39.0 33.0 40.0 10.0 41.0 21 33.70688433490685 39.0 33.0 40.0 8.0 41.0 22 33.7208934610956 39.0 34.0 40.0 2.0 41.0 23 33.69004910821193 39.0 34.0 40.0 2.0 41.0 24 32.41320771428665 38.0 32.0 40.0 2.0 41.0 25 32.95150195022258 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6781521271867632 1101 2 -0.8917798866018138 1101 3 -2.0349319879699905 1101 4 -1.2257403299631058 1101 5 -1.4710444241514793 1101 6 -3.4761468458844824 1101 7 -1.4432615428995454 1101 8 -1.038879119438871 1101 9 -0.9217391157480037 1101 10 -9.923851882387908 1101 11 -4.981126499629067 1101 12 -2.3483601860661807 1101 13 -1.7430728858473117 1101 14 -1.402992939423278 1101 15 -2.46276727203853 1101 16 -1.299304266387523 1101 17 -1.178346620021884 1101 18 -1.0681747865128344 1101 19 -1.8029652321171383 1101 20 -1.1276047860792389 1101 21 -1.236458217149476 1101 22 -0.845941472539522 1101 23 -1.203363094374275 1101 24 -1.0393213528871428 1101 25 -1.0200996990737394 1102 1 -0.05669081619915062 1102 2 -0.11176396625750229 1102 3 -0.858512986094631 1102 4 -0.19918966021703355 1102 5 -0.19166568553826835 1102 6 -1.0670038093179777 1102 7 -0.4074260498533526 1102 8 0.5688205379916269 1102 9 0.4166332068928611 1102 10 0.5450832084794826 1102 11 0.24426902525781458 1102 12 0.39448700438553885 1102 13 0.08933298726337568 1102 14 -0.2076645424596677 1102 15 0.5907817504793158 1102 16 0.04383066772948041 1102 17 -0.014273857425131098 1102 18 0.005626680170351506 1102 19 0.4016234575698405 1102 20 0.21414171739368015 1102 21 0.37562137475984514 1102 22 0.1396958747729684 1102 23 -0.05554306540366838 1102 24 -3.2281516000694985 1102 25 -1.6953933607406668 1103 1 -0.5474960082986478 1103 2 -0.6910300433750862 1103 3 -0.4670791813948547 1103 4 -0.5044171028959745 1103 5 -0.8722154022347581 1103 6 -0.28270015061654874 1103 7 -0.43827754772221894 1103 8 -0.2306422456374122 1103 9 -0.46539001047763406 1103 10 -0.4123573994728602 1103 11 -0.677539454377694 1103 12 -0.7595658665349632 1103 13 -0.5872930161419703 1103 14 -0.8407403046012973 1103 15 -0.4246613600183551 1103 16 -0.49674640583022267 1103 17 -0.7860337866891598 1103 18 -0.8982386238616229 1103 19 -2.5351370483461118 1103 20 -1.3334853909511537 1103 21 -1.1752250634534462 1103 22 -0.959355563818562 1103 23 -0.7720212971036489 1103 24 0.9298783089435076 1103 25 0.1493148967908411 1104 1 -0.5339787249429122 1104 2 -0.48688440500257 1104 3 -1.6751810978227084 1104 4 -0.7174981812353529 1104 5 -0.9747489418355819 1104 6 -2.2360955332680987 1104 7 -1.0524220871286083 1104 8 -0.33804505053711154 1104 9 -0.254599853832687 1104 10 -0.2574961650802905 1104 11 -0.1037607861555685 1104 12 -0.20296488422831516 1104 13 -0.37280864259493285 1104 14 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1106 1 -0.02035570673974263 1106 2 -0.4997105101950865 1106 3 -0.4559964656609772 1106 4 -0.468920041817519 1106 5 0.19535423742790314 1106 6 0.4984737142782478 1106 7 -2.6405690546648906 1106 8 0.08392458288290428 1106 9 -0.3014918930911392 1106 10 0.16402048620734888 1106 11 5.677097759857475E-4 1106 12 0.10109517784701438 1106 13 -0.015489625733927426 1106 14 0.0376820482396667 1106 15 0.6105545868255007 1106 16 0.6956549912223338 1106 17 0.24965068387903244 1106 18 0.012963352456630162 1106 19 0.5242376854472255 1106 20 0.5831940536352676 1106 21 0.11990228981343876 1106 22 -0.17717650256973627 1106 23 -0.06121969560437179 1106 24 -3.041567299711403 1106 25 -1.4479080353158125 1107 1 0.40811034775370914 1107 2 0.2584860877275368 1107 3 0.6163052052708338 1107 4 0.7221231615544568 1107 5 0.4256116512469603 1107 6 0.36910445340213727 1107 7 -2.07382210298643 1107 8 -1.4572315212868965 1107 9 -0.27273982324317814 1107 10 0.23466627877095192 1107 11 -0.06763942555443947 1107 12 0.0941555421402569 1107 13 -0.197455604960183 1107 14 -0.1713500155869241 1107 15 -1.6710651632874018 1107 16 -0.7144308133840198 1107 17 -0.5257971559523682 1107 18 -0.5434418861403927 1107 19 0.28654387241094526 1107 20 0.5489775851722243 1107 21 0.2541037048367727 1107 22 -0.037631116449006186 1107 23 -0.35847996957697603 1107 24 -4.36226577931399 1107 25 -2.5062250607561225 1108 1 0.8167220303379956 1108 2 0.4408267303421525 1108 3 0.820234710570201 1108 4 0.8795130066155323 1108 5 1.054694896768563 1108 6 -2.6041717883672533 1108 7 -0.891729005824839 1108 8 0.7212465202048435 1108 9 0.7133229217236732 1108 10 1.2627994849863526 1108 11 1.2150162242244988 1108 12 1.2194915962434294 1108 13 1.0910630808187776 1108 14 0.9541655647231835 1108 15 -0.3238369475660363 1108 16 0.9164934120607597 1108 17 1.07728601151436 1108 18 1.30616644565972 1108 19 1.5486577098672498 1108 20 -0.01912584868463796 1108 21 0.6124948824060326 1108 22 0.9778906524974218 1108 23 1.2491181147334416 1108 24 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12 0.9855724924994078 1110 13 0.7087484430973419 1110 14 0.6217836315173528 1110 15 1.2705222196046648 1110 16 1.1154057862563462 1110 17 0.7311289378598573 1110 18 0.6261365849913432 1110 19 -1.5093744284944464 1110 20 0.21295039410221506 1110 21 0.4196668918437467 1110 22 0.5425188381348747 1110 23 0.30218836903953417 1110 24 -0.4713360891094389 1110 25 -0.16849517217482912 1111 1 0.3266931207214583 1111 2 -0.1926866062639654 1111 3 0.7869286504600161 1111 4 0.49547329680262564 1111 5 0.532014484397429 1111 6 0.8040701796685283 1111 7 0.6706012183610532 1111 8 1.2814548876297032 1111 9 0.2544727608116588 1111 10 0.3541756918264767 1111 11 0.32145815953241197 1111 12 0.36083234789346363 1111 13 0.31474714780181046 1111 14 0.43590341450227044 1111 15 -0.25115380787259056 1111 16 -0.07234093038183431 1111 17 0.19374267436277393 1111 18 0.07937323123764628 1111 19 0.6482322475861153 1111 20 0.470630328700409 1111 21 0.3991364577073995 1111 22 0.6200932379989723 1111 23 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2215 9 0.3005586879068076 2215 10 0.08054080799082897 2215 11 0.14993939072661533 2215 12 -0.008831737343058421 2215 13 0.4364163630141604 2215 14 0.42415872945318966 2215 15 0.2646899251713606 2215 16 0.682352504235638 2215 17 0.11446481711421796 2215 18 -0.5626925705677124 2215 19 0.5154990556559653 2215 20 -3.4772560226042835 2215 21 -1.750979460015678 2215 22 -0.8309648195159482 2215 23 -0.7559197324096729 2215 24 -0.7991991415537711 2215 25 -0.5220654568416521 2216 1 -0.2345523489288439 2216 2 -0.5810364415681448 2216 3 0.42311509196142083 2216 4 -0.21429088209881897 2216 5 0.16779574502492522 2216 6 -0.9693598659021738 2216 7 -8.720554761700548 2216 8 -10.587796062164792 2216 9 -5.531194330145134 2216 10 -3.3453569412662105 2216 11 -2.1374495039245467 2216 12 -1.7525852604705605 2216 13 -1.3823796631234977 2216 14 -1.5041890872224215 2216 15 -2.464334331984251 2216 16 -1.4620792632131412 2216 17 -1.7761087593167915 2216 18 -1.4403659760847631 2216 19 -0.8553337327669794 2216 20 -1.6121752330629562 2216 21 -4.57344630315815 2216 22 -2.7470618481383973 2216 23 -2.6987677272146087 2216 24 -2.6370329085606095 2216 25 -2.0124884125257836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38960.0 3 5.0 4 303.0 5 1750.0 6 1992.0 7 1549.0 8 1309.0 9 1134.0 10 1124.0 11 1090.0 12 1088.0 13 1102.0 14 1189.0 15 1212.0 16 1242.0 17 1406.0 18 1661.0 19 1978.0 20 2605.0 21 3293.0 22 4323.0 23 4909.0 24 5721.0 25 6830.0 26 7714.0 27 8494.0 28 9348.0 29 11271.0 30 13097.0 31 16128.0 32 20852.0 33 27635.0 34 37046.0 35 51308.0 36 74350.0 37 114923.0 38 129733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9617127296094 18.724228773712813 12.66584384999318 25.648214646684604 2 13.821929864892379 19.85119770901615 43.401598696871574 22.925273729219896 3 19.910539521563784 24.367142650710036 28.902729792572547 26.819588035153636 4 11.405560397391646 16.78278866780573 39.49050753931325 32.32114339548938 5 13.045546789511588 38.072410913642706 36.398550403659094 12.483491893186601 6 31.19286825164546 37.569640853342435 18.434565487010218 12.802925408001888 7 28.18508429073394 30.877979128008736 22.33079342519115 18.606143156066167 8 23.738884118477284 39.711209151294426 20.622653862223935 15.927252868004354 9 26.095986482163507 15.78896426139952 20.203095650176767 37.9119536062602 10 14.163006328542762 30.024890846173914 35.192899955862636 20.619202869420686 11 34.31390240461434 22.59776435501281 24.78107805727897 18.30725518309388 12 22.98472693866168 24.890496579556082 32.856603835490596 19.26817264629165 13 29.094267566077846 22.422996666579177 25.614845534003518 22.86789023333946 14 19.942090800967886 23.080835007997376 27.73883443382685 29.23823975720789 15 22.500918659282394 32.45196724323473 23.140863277251373 21.906250820231502 16 21.112665959055914 28.686288373802217 29.39302418289824 20.80802148424363 17 21.01737790923721 29.283573421789566 28.654274263534607 21.044774405438616 18 20.699232098007982 28.42123221940244 30.903092769975544 19.976442912614033 19 23.700322868542628 26.99807722595548 27.086012967240652 22.215586938261232 20 22.80682183564862 28.35628933087478 28.699480947287604 20.137407886189 21 22.11539092489911 27.339315594343645 27.22120148298829 23.324091997768956 22 22.223826264700914 29.300278392508826 27.651249813393353 20.824645529396903 23 21.695493045085954 28.60545729851748 28.105103770040802 21.59394588635577 24 23.135891702661336 27.71034530275714 28.616869620280163 20.536893374301364 25 22.28459688505819 28.03103130362316 28.4523063496386 21.232065461680047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 169.0 2 222.0 3 275.0 4 275.0 5 275.0 6 408.0 7 541.0 8 541.0 9 541.0 10 835.0 11 1129.0 12 1129.0 13 1129.0 14 1928.5 15 2728.0 16 2728.0 17 2728.0 18 4835.5 19 6943.0 20 6943.0 21 6943.0 22 11222.0 23 15501.0 24 15501.0 25 15501.0 26 22981.0 27 30461.0 28 30461.0 29 30461.0 30 40848.5 31 51236.0 32 51236.0 33 51236.0 34 57853.5 35 64471.0 36 64471.0 37 64471.0 38 68934.0 39 73397.0 40 73397.0 41 73397.0 42 76158.5 43 78920.0 44 78920.0 45 78920.0 46 79767.5 47 80615.0 48 80615.0 49 80615.0 50 76171.5 51 71728.0 52 71728.0 53 71728.0 54 62372.5 55 53017.0 56 53017.0 57 53017.0 58 44646.0 59 36275.0 60 36275.0 61 36275.0 62 29076.5 63 21878.0 64 21878.0 65 21878.0 66 16752.0 67 11626.0 68 11626.0 69 11626.0 70 8511.5 71 5397.0 72 5397.0 73 5397.0 74 3772.5 75 2148.0 76 2148.0 77 2148.0 78 1475.5 79 803.0 80 803.0 81 803.0 82 542.5 83 282.0 84 282.0 85 282.0 86 190.0 87 98.0 88 98.0 89 98.0 90 61.5 91 25.0 92 25.0 93 25.0 94 17.0 95 9.0 96 9.0 97 9.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19600639030039005 2 0.11120697290683218 3 0.11317523791403274 4 0.06577285565728569 5 0.0205027604916726 6 0.01935460590413893 7 0.29163126523355104 8 0.2886788677227502 9 0.017878407148738507 10 0.03493670387781011 11 0.015746120057604555 12 0.017058296729071602 13 0.014761987554004272 14 0.016238186309404698 15 0.014761987554004272 16 0.01820645131660527 17 0.017550362980871745 18 0.014926009637937653 19 0.022635047582806548 20 0.01673025256120484 21 0.015418075889737795 22 0.01689427464513822 23 0.017222318813004983 24 0.015254053805804413 25 0.015746120057604555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 609674.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.87623421393316 #Duplication Level Percentage of deduplicated Percentage of total 1 93.84529651323027 79.65235366732938 2 4.595396420347927 7.800798857586414 3 0.7615460481598892 1.939114822449419 4 0.25313820643775015 0.8594167079242179 5 0.1418348906415018 0.6019205698897852 6 0.08938643683401916 0.4552070488963892 7 0.0532796662215848 0.3165524200344391 8 0.03376250648690134 0.22925075265853415 9 0.028325787839334562 0.2163767582650986 >10 0.1624506574574621 2.5763186554069595 >50 0.018565045062765063 1.07051069776675 >100 0.014890593620725501 2.6288236243061736 >500 0.0013536903290417889 0.7789958117136145 >1k 7.735373308810222E-4 0.874359605772951 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1620 0.26571577597207685 No Hit GTCCTACAGTGTGCATTTCTCATTT 1295 0.21240859869372813 No Hit CTGTAGGACGTGGAATATGGCAAGA 1214 0.1991228098951243 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1198 0.19649845655219017 No Hit CTTTAGGACGTGAAATATGGCGAGG 890 0.14597965470070892 No Hit CTGTAGGACCTGGAATATGGCGAGA 773 0.12678907088050337 No Hit GTATCAACGCAGAGTACTTTTTTTT 692 0.1135032820818995 No Hit GATATACACTGTTCTACAAATCCCG 648 0.10628631038883075 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.640220839333808E-4 13 0.0 0.0 0.0 0.0 1.640220839333808E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAG 35 0.0021086961 16.353634 1 GTTTATA 65 3.2217267E-6 14.676339 1 CCAACGA 75 9.847772E-7 13.907641 19 GTATTAT 70 6.9401867E-6 13.628028 1 TAGGACC 310 0.0 13.516677 4 CTATACT 85 2.6114503E-7 13.444395 4 GTCTAAA 50 0.0014514595 13.355469 1 GGTCTAC 80 1.9026375E-6 13.116978 1 CTGTAGG 765 0.0 13.093596 1 TGTAGGA 800 0.0 12.868927 2 TCTAAAG 60 3.9694598E-4 12.710052 2 TCCAACG 105 2.0361767E-8 12.643311 18 GTAGGAC 785 0.0 12.507663 3 GACGTGG 370 0.0 12.347187 7 ATTATAC 55 0.0029872428 12.132322 3 TAGCACA 110 3.669993E-8 12.120325 4 AGACTTA 55 0.003022497 12.113338 6 AGGACGT 630 0.0 12.085871 5 CTAGGAC 80 2.7612685E-5 11.915673 3 GGACGTG 640 0.0 11.748317 6 >>END_MODULE