FastQCFastQC Report
Thu 26 May 2016
SRR1926873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609674
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16220.26604382013994365No Hit
CTGTAGGACGTGGAATATGGCAAGA14560.23881615420700245No Hit
GTCCTACAGTGTGCATTTCTCATTT13120.2151969741205956No Hit
CTTTAGGACGTGAAATATGGCGAGG12450.2042074944970591No Hit
GTCCTAAAGTGTGTATTTCTCATTT11760.1928899707056558No Hit
GTATCAACGCAGAGTACTTTTTTTT11650.19108572778238864No Hit
CTGTAGGACCTGGAATATGGCGAGA10080.16533426060484785No Hit
GATATACACTGTTCTACAAATCCCG7610.12482080587330277No Hit
GTACATGGGAAGCAGTGGTATCAAC7530.12350862920183575No Hit
GGTATCAACGCAGAGTACTTTTTTT7310.11990014335530134No Hit
CTGAAGGACCTGGAATATGGCGAGA6400.1049741337173637No Hit
CCCATGTACTCTGCGTTGATACCAC6100.10005347119936228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA350.0021248116.3350166
CCAACGA653.4109325E-614.59588319
TAGGACC3800.014.2931394
GCCGGTT602.601717E-514.22748411
CCGGTTT602.6037304E-514.226315512
TCCAACG954.976755E-913.98017118
GAGTAAG551.8915602E-413.8737341
CAATACA551.9075091E-413.8600144
AAGAGGT551.982124E-413.797487
GATATAC2850.013.7216091
ACCCTAC500.001453100513.3534691
TCTTATG500.001455813613.3501652
TGCCGGT655.5232245E-513.13306110
CCTACAC1155.0622475E-912.4288173
ACAGATC1001.4667239E-712.3304868
ACAAGAC550.002996133212.1275123
CTGTAGG9300.012.1022221
GGACGTG7000.011.97901256
GACGTGG4050.011.945047
TAAATAC802.7725084E-511.910953