FastQCFastQC Report
Thu 26 May 2016
SRR1926872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595893
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9240.15506139525048962No Hit
GTATCAACGCAGAGTACTTTTTTTT7750.13005690618953403No Hit
CTGTAGGACGTGGAATATGGCAAGA7250.12166613804827377No Hit
GTCCTACAGTGTGCATTTCTCATTT6830.11461789280961515No Hit
GTACATGGGAAGCAGTGGTATCAAC6820.11445007744678995No Hit
GTCCTAAAGTGTGTATTTCTCATTT6320.10605930930552969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA250.005916004519.0616662
TAGACTC350.00214085416.3165475
CTACACT1058.54925E-1114.504824
TTAAGGG400.005164723414.2950453
GTGCGAG400.005164723414.2950453
CTCGTGG400.005337699314.22425311
TAGGACC1800.013.7493614
GTATTAG603.9441983E-412.7195811
GTGTAGT603.9441983E-412.7195811
ATAATAC701.057098E-412.2528943
TCTAGCA550.00299111312.1301522
TAATAGT550.003019588512.1148214
GTCCTAT959.852738E-712.050131
TAAGGGA657.8789226E-411.7154324
GTCCTAA2500.011.4476231
ATGCCGG855.4039465E-511.15721319
AGGACGT3600.011.1043185
TTAGGAC1552.928573E-1011.0671313
TGTAGGA5600.011.0625742
GGACGTG3450.011.0353466
Produced by FastQC (version 0.11.5)