FastQCFastQC Report
Thu 26 May 2016
SRR1926872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595893
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14150.23745873839766535No Hit
GTCCTACAGTGGACATTTCTAAATT9250.15522921061331482No Hit
GGTATCAACGCAGAGTACTTTTTTT8760.14700625783487975No Hit
CTGTAGGACGTGGAATATGGCAAGA8360.14029364332187155No Hit
GTACATGGGAAGCAGTGGTATCAAC8300.1392867511449203No Hit
TATCAACGCAGAGTACTTTTTTTTT7540.12653278357020473No Hit
GTCCTACAGTGTGCATTTCTCATTT7090.11898109224307049No Hit
CCCATGTACTCTGCGTTGATACCAC6820.11445007744678995No Hit
CTTTAGGACGTGAAATATGGCGAGG6680.11210066236723706No Hit
GTCCTAAAGTGTGTATTTCTCATTT6670.11193284700441186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2100.013.6004764
CGTGGAA2900.013.4168559
AGGACGT4900.013.2118915
GGACGTG4900.013.2118916
GTCCTAC5200.013.19755651
TGAGTTA2350.012.92034412
TAGGACG5200.012.8158334
GACGTGA2150.012.803657
GACGTGG3000.012.6564817
ACGTGGA3150.012.65329558
CTGTAGG5500.012.6509911
AGGACCT3850.012.611355
TCCTACA5650.012.481722
TGTAGGA5650.012.481722
GTAGGAC5500.012.465083
GGACCTG3700.012.3507036
GTATTGG701.0555073E-412.2548731
AGACTGC802.7974942E-511.9004166
TAGAATA1052.6346424E-711.787084
GGGTAGA657.7907124E-411.7311611
Produced by FastQC (version 0.11.5)