##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926872_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 595893 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.947737261555346 31.0 30.0 33.0 26.0 34.0 2 30.241202699142296 31.0 30.0 34.0 26.0 34.0 3 30.30222875583368 31.0 30.0 34.0 26.0 34.0 4 33.90408512937725 35.0 35.0 37.0 30.0 37.0 5 33.89295393636106 37.0 35.0 37.0 30.0 37.0 6 33.84210252511777 37.0 35.0 37.0 30.0 37.0 7 33.83195640828135 37.0 35.0 37.0 29.0 37.0 8 33.86662370593378 37.0 35.0 37.0 30.0 37.0 9 35.40875291369424 39.0 35.0 39.0 30.0 39.0 10 35.310945085778826 39.0 35.0 39.0 30.0 39.0 11 35.33807411733314 39.0 35.0 39.0 30.0 39.0 12 35.2390647314199 39.0 35.0 39.0 29.0 39.0 13 35.247156788215335 39.0 35.0 39.0 30.0 39.0 14 36.361183970947806 40.0 36.0 41.0 30.0 41.0 15 36.333225931501126 40.0 36.0 41.0 29.0 41.0 16 36.31260645787079 40.0 36.0 41.0 29.0 41.0 17 36.2938782633795 40.0 36.0 41.0 29.0 41.0 18 36.26592693654733 40.0 36.0 41.0 29.0 41.0 19 36.32738260056755 40.0 37.0 41.0 29.0 41.0 20 36.29302240502909 40.0 37.0 41.0 29.0 41.0 21 36.234322269266464 40.0 37.0 41.0 29.0 41.0 22 36.14537509250822 40.0 37.0 41.0 29.0 41.0 23 36.074562043856865 40.0 37.0 41.0 29.0 41.0 24 35.99963919696992 40.0 36.0 41.0 29.0 41.0 25 35.882779290912964 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1179875970855555 1101 2 -0.1933305220792505 1101 3 0.11630066287333918 1101 4 0.07482271901260873 1101 5 -0.06806845219547597 1101 6 -0.1529298533324308 1101 7 0.04050803618660126 1101 8 0.11248728149961096 1101 9 0.08644215762972607 1101 10 0.2416083718351132 1101 11 0.06544187489308939 1101 12 0.10455550492616794 1101 13 0.20166420108954952 1101 14 0.12906039576562023 1101 15 0.09180859843626621 1101 16 0.08025307072684029 1101 17 0.14024342499910603 1101 18 -0.10688901075022983 1101 19 0.0702230685419849 1101 20 -0.005971558106480757 1101 21 0.25262180609692564 1101 22 0.0580686046992156 1101 23 0.19082120475331976 1101 24 0.2998962913811525 1101 25 0.16626962564428993 1102 1 -0.5317374651037134 1102 2 -0.3605105981007881 1102 3 -0.195412725364438 1102 4 -0.4537077951886417 1102 5 -0.5088653585411649 1102 6 -0.6820978398174873 1102 7 -0.34098810994368733 1102 8 -0.3041186735209749 1102 9 -0.3988431871068414 1102 10 -0.11347820825146471 1102 11 -0.3421114457699872 1102 12 -0.04376304644116402 1102 13 -0.42755657813123094 1102 14 -0.23518440414991204 1102 15 -0.5050924679769935 1102 16 -0.22130827443206158 1102 17 -0.27767047004893897 1102 18 0.007126140764924571 1102 19 -0.05563481341345522 1102 20 -0.09689516701789103 1102 21 -0.36067272457565025 1102 22 -0.108357855019932 1102 23 -0.1568229194151911 1102 24 -0.12789512452002327 1102 25 -0.3398754487934639 1103 1 -0.9236694152673728 1103 2 -0.8410577948065239 1103 3 -0.7041538825812061 1103 4 -0.7967681900783035 1103 5 -0.8828411794682012 1103 6 -1.0142934896960654 1103 7 -0.8163101456315829 1103 8 -0.7977399912276582 1103 9 -1.0317396605520983 1103 10 -0.6879370827103415 1103 11 -0.6402399432887336 1103 12 -0.6795354041647386 1103 13 -0.668790344364993 1103 14 -0.6936668769616503 1103 15 -0.7797823106546389 1103 16 -0.6174742020004302 1103 17 -0.7199838477281659 1103 18 -0.8887071925684111 1103 19 -0.6627314769125618 1103 20 -0.7548351944701182 1103 21 -0.8371509211757981 1103 22 -0.8930677589371498 1103 23 -0.9307697043375853 1103 24 -1.011467344982485 1103 25 -1.1860031016284367 1104 1 -0.4631232520764428 1104 2 -0.5608163491697553 1104 3 -0.24690798260145286 1104 4 -0.09103322348587284 1104 5 -0.3593900760059654 1104 6 -0.22718577558389796 1104 7 -0.3644018646030318 1104 8 -0.2775866200999033 1104 9 -0.27061139572116133 1104 10 -0.29032623031107363 1104 11 -0.3707167604582011 1104 12 -0.21049308790638577 1104 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-0.5248719130512285 2216 25 -0.573983653414956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13168.0 3 1.0 4 222.0 5 1410.0 6 2005.0 7 1534.0 8 1171.0 9 1063.0 10 874.0 11 823.0 12 745.0 13 719.0 14 755.0 15 691.0 16 774.0 17 754.0 18 861.0 19 1064.0 20 1364.0 21 1768.0 22 2256.0 23 2803.0 24 3326.0 25 3999.0 26 4852.0 27 5133.0 28 6026.0 29 7492.0 30 9644.0 31 12267.0 32 16085.0 33 21443.0 34 28819.0 35 39849.0 36 58966.0 37 101741.0 38 239426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.07985081522424 18.92529841155174 10.708711685300763 28.286139087923257 2 14.44294197189723 20.288250758002064 39.816903656047636 25.451903614053062 3 18.794026864674613 24.013554861438635 29.129021267168064 28.063397006718688 4 12.250521486239979 17.082261412703286 37.16170520546474 33.505511895591994 5 13.286275536490772 38.242683301362895 34.922457007735 13.548584154411333 6 32.58765445403509 37.451041143961774 17.160100089919275 12.801204312083872 7 28.72932349757043 31.497939693010064 20.831843702006598 18.940893107412908 8 25.48855397422127 38.087238184747584 19.006780343716436 17.417427497314716 9 26.214120162526033 15.8639104596345 19.367182294664868 38.554787083174595 10 14.896412838263059 29.76614222781624 33.41086637298091 21.926578560939795 11 34.8495477201403 22.80614899222985 23.518049238927954 18.826254048701898 12 23.48138663408789 24.825093264013937 31.002753868589622 20.69076623330855 13 29.15082893233113 21.86505159535281 25.318223085920653 23.665896386395406 14 20.91180799237447 22.241744742764222 26.87294531065141 29.9735019542099 15 23.24803192551608 30.64574323655324 22.989090213412947 23.117134624517735 16 22.443647128319416 28.34542732862551 27.080424509303764 22.130501033751308 17 21.75767044644071 28.8398381583209 27.223435158443404 22.179056236794985 18 21.67823483607658 27.99368337674194 28.82152350777327 21.50655827940821 19 23.440153584877795 26.9793855262363 27.03812100179227 22.542339887093636 20 23.313105818543082 27.594615095454177 27.651840614343637 21.440438471659103 21 23.016993244660764 26.713548441236938 26.985943859354677 23.28351445474762 22 23.024267779618153 27.83503078572831 26.862540758156246 22.27816067649729 23 22.444969147145542 27.654629270691217 27.183068101152386 22.71733348101085 24 22.836185080517946 27.031408376014443 27.830378659220127 22.30202788424748 25 22.79917093923857 27.140831242500273 27.518775541029278 22.541222277231874 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 215.0 1 215.0 2 216.5 3 218.0 4 218.0 5 218.0 6 346.0 7 474.0 8 474.0 9 474.0 10 759.5 11 1045.0 12 1045.0 13 1045.0 14 1632.0 15 2219.0 16 2219.0 17 2219.0 18 4032.0 19 5845.0 20 5845.0 21 5845.0 22 9496.5 23 13148.0 24 13148.0 25 13148.0 26 18786.5 27 24425.0 28 24425.0 29 24425.0 30 32922.0 31 41419.0 32 41419.0 33 41419.0 34 47499.0 35 53579.0 36 53579.0 37 53579.0 38 58555.0 39 63531.0 40 63531.0 41 63531.0 42 68482.0 43 73433.0 44 73433.0 45 73433.0 46 76956.5 47 80480.0 48 80480.0 49 80480.0 50 78522.5 51 76565.0 52 76565.0 53 76565.0 54 68486.0 55 60407.0 56 60407.0 57 60407.0 58 51920.5 59 43434.0 60 43434.0 61 43434.0 62 35791.5 63 28149.0 64 28149.0 65 28149.0 66 22024.5 67 15900.0 68 15900.0 69 15900.0 70 11543.5 71 7187.0 72 7187.0 73 7187.0 74 4995.0 75 2803.0 76 2803.0 77 2803.0 78 1893.0 79 983.0 80 983.0 81 983.0 82 649.5 83 316.0 84 316.0 85 316.0 86 203.5 87 91.0 88 91.0 89 91.0 90 56.5 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11042250873898503 2 0.0969972797129686 3 0.015439013379918879 4 0.0 5 0.005034460884756156 6 0.3401617404466909 7 0.018124059185122162 8 0.008894214229735875 9 0.00839076814126026 10 0.0030206765308536934 11 0.0038597533449797198 12 6.712614513008209E-4 13 0.0010068921769512313 14 0.0 15 0.0010068921769512313 16 8.390768141260259E-4 17 3.3563072565041043E-4 18 8.390768141260259E-4 19 1.6781536282520522E-4 20 8.390768141260259E-4 21 0.011411444672113953 22 0.0 23 0.0 24 1.6781536282520522E-4 25 0.006376983787357798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 595893.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.33527345894332 #Duplication Level Percentage of deduplicated Percentage of total 1 92.02508918261022 76.68935972116469 2 5.891727937571649 9.81977517646459 3 1.0114590817617806 2.5287065751344913 4 0.36486438227688944 1.2162429228989204 5 0.19177646667272938 0.7990872146580917 6 0.11711970670792411 0.5856121671561657 7 0.0814288860440872 0.4750128940158798 8 0.04987494497837526 0.3325073742818123 9 0.040054729186776 0.3004174629093507 >10 0.19718791386717266 2.908841490736795 >50 0.014506951024798553 0.8123399426983946 >100 0.012693493104868336 2.030838273236888 >500 0.0020148401752246676 1.263670016019536 >1k 2.0148401752246678E-4 0.23758876862445436 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1415 0.23745873839766535 No Hit GTCCTACAGTGGACATTTCTAAATT 925 0.15522921061331482 No Hit GGTATCAACGCAGAGTACTTTTTTT 876 0.14700625783487975 No Hit CTGTAGGACGTGGAATATGGCAAGA 836 0.14029364332187155 No Hit GTACATGGGAAGCAGTGGTATCAAC 830 0.1392867511449203 No Hit TATCAACGCAGAGTACTTTTTTTTT 754 0.12653278357020473 No Hit GTCCTACAGTGTGCATTTCTCATTT 709 0.11898109224307049 No Hit CCCATGTACTCTGCGTTGATACCAC 682 0.11445007744678995 No Hit CTTTAGGACGTGAAATATGGCGAGG 668 0.11210066236723706 No Hit GTCCTAAAGTGTGTATTTCTCATTT 667 0.11193284700441186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.678153628252052E-4 0.0 7 0.0 0.0 0.0 1.678153628252052E-4 0.0 8 0.0 0.0 0.0 1.678153628252052E-4 0.0 9 0.0 0.0 0.0 1.678153628252052E-4 0.0 10 0.0 0.0 0.0 1.678153628252052E-4 0.0 11 0.0 0.0 0.0 1.678153628252052E-4 0.0 12 0.0 0.0 0.0 1.678153628252052E-4 1.678153628252052E-4 13 0.0 0.0 0.0 1.678153628252052E-4 1.678153628252052E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 210 0.0 13.600476 4 CGTGGAA 290 0.0 13.416855 9 AGGACGT 490 0.0 13.211891 5 GGACGTG 490 0.0 13.211891 6 GTCCTAC 520 0.0 13.1975565 1 TGAGTTA 235 0.0 12.920344 12 TAGGACG 520 0.0 12.815833 4 GACGTGA 215 0.0 12.80365 7 GACGTGG 300 0.0 12.656481 7 ACGTGGA 315 0.0 12.6532955 8 CTGTAGG 550 0.0 12.650991 1 AGGACCT 385 0.0 12.61135 5 TCCTACA 565 0.0 12.48172 2 TGTAGGA 565 0.0 12.48172 2 GTAGGAC 550 0.0 12.46508 3 GGACCTG 370 0.0 12.350703 6 GTATTGG 70 1.0555073E-4 12.254873 1 AGACTGC 80 2.7974942E-5 11.900416 6 TAGAATA 105 2.6346424E-7 11.78708 4 GGGTAGA 65 7.7907124E-4 11.731161 1 >>END_MODULE