FastQCFastQC Report
Thu 26 May 2016
SRR1926871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542132
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19210.354341747028399No Hit
GTCCTAAAGTGTGTATTTCTCATTT15290.28203463363166165No Hit
CTGTAGGACGTGGAATATGGCAAGA15040.27742321058340036No Hit
CTTTAGGACGTGAAATATGGCGAGG13590.2506769569034848No Hit
GTCCTACAGTGTGCATTTCTCATTT10910.20124250182612355No Hit
GTATCAACGCAGAGTACTTTTTTTT7870.14516759755926603No Hit
CTGTAGGACCTGGAATATGGCGAGA7380.13612920838467385No Hit
GTACATGGGAAGCAGTGGTATCAAC7250.13373126839957797No Hit
CTGAAGGACCTGGAATATGGCGAGA7080.13059550072676027No Hit
GTCCTTCAGTGTGCATTTCTCATTT6130.1130720931433673No Hit
CCCATGTACTCTGCGTTGATACCAC6100.11251872237757593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA250.005903350719.0683562
GCCTCGA350.002194935216.25535416
GTGTAGA852.575889E-713.4587671
GGGCTCG500.00151855313.27520616
TAGGACC2500.012.9520664
GTATTAC603.9655456E-412.7110571
TAGCCCT853.8224443E-612.3246314
TCCAACG1103.8928192E-812.06836918
TAGGACT951.0050444E-612.0297834
GTCCTAT802.757708E-511.91661551
GGACCTG5150.011.8296466
GTACTAC657.776882E-411.7332831
CTCCTAC657.776882E-411.7332831
TAAAGGT657.8437215E-411.7213264
GGCTCGG658.134985E-411.67050917
GTGTAAG907.1511913E-611.6518031
AGGACCT5850.011.5531795
GTACAAG752.0028766E-411.4399511
ATGTAGG855.1218667E-511.2156391
GTCCTAC9800.011.1870271
Produced by FastQC (version 0.11.5)