##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926871_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542132
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.90704108962393	31.0	28.0	31.0	16.0	34.0
2	27.884365800211018	31.0	28.0	31.0	16.0	34.0
3	27.333134734714054	31.0	27.0	31.0	12.0	34.0
4	31.45303173396885	35.0	32.0	37.0	17.0	37.0
5	30.94181490854626	35.0	32.0	37.0	16.0	37.0
6	30.506675496004664	35.0	30.0	37.0	11.0	37.0
7	30.578364678712934	35.0	31.0	37.0	11.0	37.0
8	30.65494934812924	35.0	31.0	37.0	10.0	37.0
9	32.73018563744623	37.0	32.0	39.0	17.0	39.0
10	32.89379339349088	37.0	33.0	39.0	17.0	39.0
11	33.108781625139265	37.0	33.0	39.0	17.0	39.0
12	32.99683287465045	37.0	33.0	39.0	16.0	39.0
13	33.09724200010329	38.0	33.0	39.0	17.0	39.0
14	34.03731010159887	39.0	34.0	40.0	16.0	41.0
15	33.676099178797784	39.0	33.0	40.0	10.0	41.0
16	33.72829310942723	39.0	33.0	40.0	11.0	41.0
17	33.93875292364221	39.0	33.0	40.0	11.0	41.0
18	33.98427320283621	39.0	34.0	41.0	11.0	41.0
19	33.37946477979533	39.0	33.0	40.0	10.0	41.0
20	33.71190595648292	39.0	33.0	41.0	10.0	41.0
21	33.6750403960659	39.0	34.0	41.0	2.0	41.0
22	33.69613304508865	39.0	34.0	41.0	2.0	41.0
23	33.67506253089653	39.0	34.0	41.0	2.0	41.0
24	32.41843499369158	38.0	32.0	40.0	2.0	41.0
25	32.96160898083861	39.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.9957727200059345
1101	2	-1.0426585524789225
1101	3	-2.0457302679615594
1101	4	-1.265109494766726
1101	5	-1.5354073399131742
1101	6	-3.5610766673033147
1101	7	-1.6191246199353948
1101	8	-1.1361889436889925
1101	9	-1.1180153405665436
1101	10	-9.907759991816182
1101	11	-5.051925338859558
1101	12	-2.482945253346955
1101	13	-1.7473795784247699
1101	14	-1.5850088642800912
1101	15	-3.147513385769315
1101	16	-1.5648158216562749
1101	17	-1.5134309895895512
1101	18	-1.384012267946538
1101	19	-1.9338182325998012
1101	20	-1.3020126202809834
1101	21	-1.1767045850260232
1101	22	-0.9539761250791017
1101	23	-0.9790724882599307
1101	24	-1.3154244041584775
1101	25	-0.9236726037772058
1102	1	-1.268931927947751
1102	2	-1.4691728594280136
1102	3	-1.9805925071716253
1102	4	-1.6084497294113085
1102	5	-1.8790926401202128
1102	6	-2.9216967235755433
1102	7	-1.8222753339556732
1102	8	-1.3038642635382267
1102	9	-1.5287495339094335
1102	10	-1.4387229901704863
1102	11	-1.6104272234483972
1102	12	-1.6393793989115366
1102	13	-1.6115299208359843
1102	14	-1.9241817686703868
1102	15	-1.4966401044612496
1102	16	-1.822500166297143
1102	17	-2.004246936927238
1102	18	-2.0644948288638787
1102	19	-1.7540921615693819
1102	20	-2.0332861776414966
1102	21	-1.860690888577544
1102	22	-1.905202435001062
1102	23	-2.1103301195175597
1102	24	-5.539013616878126
1102	25	-4.117599449877961
1103	1	-1.3145588156799022
1103	2	-1.5343678983072628
1103	3	-1.470760234181455
1103	4	-1.4154672732709592
1103	5	-1.8490174521502887
1103	6	-1.2530057654109008
1103	7	-1.1157012063289145
1103	8	-0.9687958232066265
1103	9	-1.2011034957370796
1103	10	-1.2705782693941856
1103	11	-1.4467166650009986
1103	12	-1.7144388423920311
1103	13	-1.7024302485784162
1103	14	-1.7052369707782233
1103	15	-1.0386361201414758
1103	16	-1.288472790164505
1103	17	-1.5166818704148
1103	18	-1.610892875261925
1103	19	-2.832332634546699
1103	20	-1.9628858177674537
1103	21	-1.624009441369779
1103	22	-1.847269140225663
1103	23	-1.8855052367979432
1103	24	-0.39069410013755856
1103	25	-1.0670564256507262
1104	1	-0.8253763005685961
1104	2	-0.8450526985431459
1104	3	-2.251452774355421
1104	4	-1.1114259198874983
1104	5	-1.2772018838176926
1104	6	-2.3925273635238327
1104	7	-1.2570011473579576
1104	8	-0.5525714387159901
1104	9	-0.7779684522459966
1104	10	-0.8572840043197374
1104	11	-0.8267015536933187
1104	12	-0.7225197063165467
1104	13	-1.0606788111613454
1104	14	-1.102627505939651
1104	15	-0.8757564946070531
1104	16	-1.2724032044354772
1104	17	-1.1196046184613877
1104	18	-1.1492332113963784
1104	19	-1.7762272976751063
1104	20	-1.1066090965526527
1104	21	-1.0720228572624535
1104	22	-0.9879701574746704
1104	23	-0.8863437300899975
1104	24	0.25464166574774083
1104	25	-0.27265313801988356
1105	1	-0.12480043915606842
1105	2	-0.08098930619591371
1105	3	0.3984972541996896
1105	4	-0.02263142150562203
1105	5	-0.2568087751179
1105	6	-0.36344874178387343
1105	7	0.060281833795666984
1105	8	0.39424466636682
1105	9	-0.623485299761704
1105	10	0.04377253480076604
1105	11	-0.1268514680279864
1105	12	-0.27764296921394305
1105	13	-0.24319356463554698
1105	14	-0.4286078980965158
1105	15	0.006917691572269291
1105	16	-0.41570227163516904
1105	17	-0.4020384657867311
1105	18	-0.28143147905295507
1105	19	-0.9570406424304849
1105	20	-0.2828179296004336
1105	21	-0.4617287396153955
1105	22	-0.3458608865818462
1105	23	-0.6782364580549682
1105	24	-5.7457747629596625
1105	25	-3.1153263723621656
1106	1	-0.7583608635869581
1106	2	-1.5041027488811274
1106	3	-1.1391181705872633
1106	4	-1.5560571342314269
1106	5	-0.9389366266145664
1106	6	-0.7094708891003201
1106	7	-3.767563134989196
1106	8	-0.9794734953821767
1106	9	-1.2191988563880933
1106	10	-0.8036560739299716
1106	11	-1.1660424856651268
1106	12	-0.9616461025963332
1106	13	-1.0766910840411583
1106	14	-1.4142435949180339
1106	15	-0.7017578609237489
1106	16	-0.7015225294270877
1106	17	-0.9822957829467427
1106	18	-1.1892489120531664
1106	19	-0.4537915385303748
1106	20	-0.8270235459509934
1106	21	-1.1261541674882594
1106	22	-1.368747040354961
1106	23	-1.554595443978485
1106	24	-4.355793278401066
1106	25	-2.4063214586488684
1107	1	-0.40895357033906166
1107	2	-0.49131001663625895
1107	3	-0.2568368000035939
1107	4	-0.4525594111987168
1107	5	-0.20870220906627424
1107	6	-0.3695857224124097
1107	7	-2.6372806288187896
1107	8	-2.335607401916434
1107	9	-1.0231942676906591
1107	10	-0.7777258777515712
1107	11	-0.7415503131022945
1107	12	-0.2230830833261166
1107	13	-0.7814993668717776
1107	14	-0.7040818317458033
1107	15	-2.2162394887525814
1107	16	-1.2940554672363334
1107	17	-1.050338286336121
1107	18	-1.3402993297868662
1107	19	-0.12748995913779737
1107	20	-0.32128477725147064
1107	21	-0.7389799244495165
1107	22	-0.674767388215308
1107	23	-0.6195411559797819
1107	24	-4.786473239930167
1107	25	-2.5993943795401897
1108	1	0.7541376604928622
1108	2	0.3732058062722068
1108	3	0.9136592952073883
1108	4	0.9077878610312311
1108	5	1.1039125761005515
1108	6	-2.2279652489253436
1108	7	-0.6416361851186458
1108	8	0.8415427440595948
1108	9	0.6898916599967819
1108	10	0.8727703849271293
1108	11	0.8934579765498754
1108	12	0.9794956404582038
1108	13	0.9173922759307374
1108	14	0.9475699582050154
1108	15	-0.0495379329774579
1108	16	1.068205123701432
1108	17	1.0243969751088997
1108	18	0.9902345014838261
1108	19	0.8650158603654958
1108	20	-0.3438691949382928
1108	21	0.24351272092641807
1108	22	0.6971351567358255
1108	23	0.8558313201421086
1108	24	-0.2766191601373791
1108	25	0.43627345381826643
1109	1	0.6593760214128288
1109	2	0.567804198601678
1109	3	-0.2387635758690081
1109	4	0.6501968871300434
1109	5	1.1504812947168794
1109	6	1.0962841259842513
1109	7	1.2149587769626464
1109	8	1.5374698409537721
1109	9	0.7780944992503933
1109	10	1.0437909870803352
1109	11	0.7273017142805358
1109	12	0.5876497123281013
1109	13	0.9838688324575102
1109	14	0.8972692948859375
1109	15	-0.5781515754464053
1109	16	0.7850443359842032
1109	17	0.573042440362137
1109	18	0.9363920036019024
1109	19	-0.6175456671281552
1109	20	0.3994366550813382
1109	21	0.7830081024898661
1109	22	0.7760391304510321
1109	23	0.7337930088160931
1109	24	-0.254186163629619
1109	25	0.15758852004685053
1110	1	0.549053703991369
1110	2	0.579058108570397
1110	3	0.8387490040303511
1110	4	0.47743066925978894
1110	5	0.690730172725857
1110	6	0.6333846291802274
1110	7	0.8882621905353396
1110	8	1.0991385476041202
1110	9	0.809959044334029
1110	10	0.8265395250920307
1110	11	0.46890378146400025
1110	12	0.5399004943218486
1110	13	0.4019294879722608
1110	14	0.256857218182553
1110	15	0.8059637516309763
1110	16	0.5619789205075278
1110	17	-0.04168624180840652
1110	18	0.19248475485989047
1110	19	-1.5906994818976337
1110	20	0.028764820456011364
1110	21	0.03827126038460449
1110	22	0.18707544216053407
1110	23	0.2835890104480825
1110	24	-0.7644132818126756
1110	25	-0.20811255434455234
1111	1	0.5619466841808602
1111	2	0.25625449099933917
1111	3	0.8531223890432713
1111	4	0.9140370430754601
1111	5	0.9760660900385183
1111	6	0.9740984534696331
1111	7	0.906942207761869
1111	8	1.1072188662949038
1111	9	0.5249999573400572
1111	10	0.4897339695364735
1111	11	0.2196628253916515
1111	12	0.6020990300668956
1111	13	0.3683108402547788
1111	14	0.4683828426442247
1111	15	-0.06679679961794704
1111	16	-0.08197564452262185
1111	17	0.16818132925102702
1111	18	0.4056415162724676
1111	19	0.9796278221505972
1111	20	0.42096443285911533
1111	21	0.3820212603846045
1111	22	0.5277569667083384
1111	23	0.3195386228511836
1111	24	0.3471661755516635
1111	25	0.3590870580585488
1112	1	0.33861032211786224
1112	2	0.5742711057449235
1112	3	-1.0636091789733442
1112	4	0.20496816110938454
1112	5	0.28356868609251507
1112	6	1.2567762831684917
1112	7	1.0329589955777223
1112	8	1.3047677586451982
1112	9	0.15015139382327192
1112	10	0.5625208335032426
1112	11	0.5569226903968385
1112	12	0.7446440283361895
1112	13	0.35291840342542713
1112	14	0.6368126626511454
1112	15	0.8574416912093312
1112	16	0.7785405375473005
1112	17	-0.04790771540110228
1112	18	0.366354565770564
1112	19	1.1455589401845216
1112	20	0.6644418471893303
1112	21	0.7508880828145124
1112	22	0.7182099781309788
1112	23	0.4552473455926176
1112	24	1.889319602342944
1112	25	0.6726579303326901
1113	1	-0.4932891816172962
1113	2	0.15646431186868526
1113	3	-4.66172948368628
1113	4	-1.2130559461933998
1113	5	0.17137173954371576
1113	6	0.9826439564133871
1113	7	1.2608009283765078
1113	8	1.5496734051065424
1113	9	1.1291457463082821
1113	10	1.0833038993874098
1113	11	0.7351684310536797
1113	12	0.6681325805282938
1113	13	0.2544503972300163
1113	14	-0.0051298823129783955
1113	15	0.7970127540168619
1113	16	0.8739371806475305
1113	17	0.68119726937379
1113	18	0.5953936979919519
1113	19	1.4974051823744077
1113	20	0.8346364546554312
1113	21	0.6479104937555178
1113	22	0.8774414524906291
1113	23	0.6346692128549378
1113	24	2.108733725344976
1113	25	1.3196869265290445
1114	1	0.6882458149442776
1114	2	0.6363565318156645
1114	3	0.9042845205625447
1114	4	0.3756772547156757
1114	5	1.2002724392448663
1114	6	1.8641919908845956
1114	7	1.9276810588578606
1114	8	1.5973218519336037
1114	9	0.8642384305071005
1114	10	1.0164488221441843
1114	11	0.6640364081580898
1114	12	0.15505632409561798
1114	13	0.5308911501565134
1114	14	0.5937843921529193
1114	15	-1.8803833453473509
1114	16	0.13275886039045304
1114	17	0.36170457188141114
1114	18	0.8097420453729924
1114	19	1.140814519051581
1114	20	1.1112280383012916
1114	21	0.8472281764843785
1114	22	0.9284797558239859
1114	23	0.09462365686478336
1114	24	1.174051096186588
1114	25	0.9889765138408606
1115	1	-2.6069716659874906
1115	2	-0.12947783223298615
1115	3	0.621809257730142
1115	4	1.2616801407185605
1115	5	1.9221519486243785
1115	6	2.368479766600945
1115	7	2.2583731841928447
1115	8	2.4410782939043294
1115	9	1.8974326172727203
1115	10	0.6413122523647559
1115	11	1.3578572698360958
1115	12	0.9793296697975364
1115	13	1.2993924900864258
1115	14	1.348664829176208
1115	15	1.959002863681718
1115	16	1.7132474194504397
1115	17	1.7306181979378934
1115	18	1.7275270381579872
1115	19	-0.6443199892972089
1115	20	0.718881099525781
1115	21	1.1859985331118779
1115	22	1.6583756535770249
1115	23	1.2918871076996652
1115	24	-0.06325049111499936
1115	25	0.878594633816121
1116	1	0.9035541103781952
1116	2	0.3706694978889793
1116	3	0.8816752513757287
1116	4	0.6990071879666928
1116	5	1.7429613675031312
1116	6	2.29042298685307
1116	7	2.031939598049565
1116	8	-1.0929421529543326
1116	9	0.3501153066239553
1116	10	0.3178022082938341
1116	11	0.794920403996187
1116	12	0.9691472366725975
1116	13	0.5633568233855968
1116	14	0.8855127332450863
1116	15	0.22655052177334056
1116	16	0.629292310298041
1116	17	0.7308026808732251
1116	18	0.8005797182850785
1116	19	1.3158944721151116
1116	20	1.1052114264705395
1116	21	0.9940209785356728
1116	22	1.0510603927386555
1116	23	1.2502272737343105
1116	24	2.0636378967091247
1116	25	1.5665865883103294
1201	1	0.10245594344011977
1201	2	-0.27430106455621583
1201	3	-0.09425415416660599
1201	4	0.017123462828550373
1201	5	-0.46127958897382726
1201	6	0.2923854905066676
1201	7	0.3033928250458189
1201	8	0.9333762737023115
1201	9	0.40077156227832944
1201	10	0.31481113003030003
1201	11	0.43470912168794484
1201	12	0.34145088191874606
1201	13	0.3310423217362626
1201	14	-0.044965922787874035
1201	15	0.9254871509993379
1201	16	0.3029780591810791
1201	17	0.11872145270781687
1201	18	-0.15516095286333353
1201	19	-0.8691376099481616
1201	20	-5.633449186674966E-4
1201	21	0.07345644556978925
1201	22	0.22119832473303092
1201	23	-0.03709717961795178
1201	24	-0.4934974046952476
1201	25	-0.17756417650935674
1202	1	-0.6629139604688277
1202	2	-0.7957676192721408
1202	3	-1.40609605484142
1202	4	-0.8698747024231821
1202	5	-1.6811197481926605
1202	6	-0.7226812134769105
1202	7	-1.0504057258140271
1202	8	-0.4225151340782638
1202	9	-0.5296168446004188
1202	10	-0.55948062604147
1202	11	-0.7947245941437515
1202	12	-0.3211855580476666
1202	13	-0.5013860764352103
1202	14	-0.5787968149730887
1202	15	-0.17756972619692135
1202	16	-0.45852954414012004
1202	17	-0.656764444608477
1202	18	-0.7801384072278594
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2210	24	0.9520890873393313
2210	25	0.816956886317719
2211	1	0.5568324652906895
2211	2	0.5406162749285954
2211	3	0.49616833291101514
2211	4	0.5436346337457181
2211	5	0.6880518647405545
2211	6	-1.6781386668686977
2211	7	0.38739043280351737
2211	8	0.7239568196461796
2211	9	0.751299137142702
2211	10	0.7570470222085248
2211	11	0.5633647669525885
2211	12	0.6426042844562332
2211	13	0.387418074651805
2211	14	0.5749964848331288
2211	15	1.0566973692914488
2211	16	0.4876890677675263
2211	17	0.2680828094509522
2211	18	0.32459896853441705
2211	19	0.4177605915982454
2211	20	0.4168914801486139
2211	21	0.11410747722428027
2211	22	-2.963316178235914
2211	23	-1.1303604544267856
2211	24	1.0469571213417481
2211	25	0.6775342898924563
2212	1	-0.10057282564787684
2212	2	0.09778195240151533
2212	3	-2.3498800154112978
2212	4	-1.3562531788092898
2212	5	-0.07249756473242641
2212	6	0.701944252001649
2212	7	0.8627814467730879
2212	8	0.7624828290047674
2212	9	0.578841811722917
2212	10	0.6608219852287078
2212	11	0.6265930329340463
2212	12	0.48275688040014586
2212	13	0.1750396219977972
2212	14	-0.13675140606918035
2212	15	-1.1772857053802
2212	16	0.19956963937175232
2212	17	0.3791168519035182
2212	18	0.38411742954336603
2212	19	0.9633239328398773
2212	20	-0.4030437296313991
2212	21	-0.1878107441712018
2212	22	0.14558314995309019
2212	23	0.28162100705770854
2212	24	1.5708041785888724
2212	25	1.0945891461375155
2213	1	-0.11267274456992382
2213	2	-0.11008260881233767
2213	3	0.6097843476120097
2213	4	-6.6316942814694215
2213	5	-1.3097625351968887
2213	6	-2.4772315841951524
2213	7	-0.5293964614157858
2213	8	-2.122179281853242
2213	9	-0.545723520374942
2213	10	0.06749997706943134
2213	11	0.3856821285931531
2213	12	0.4437107689243973
2213	13	0.5626179651763081
2213	14	0.24695707365489739
2213	15	-2.653818142995224
2213	16	-0.5754274649871505
2213	17	0.036319276520330845
2213	18	0.06570115217955674
2213	19	0.7762693789616506
2213	20	-0.9470793524516239
2213	21	0.2125650456953423
2213	22	0.1793694902488312
2213	23	0.4342985098568306
2213	24	0.013868152952795754
2213	25	0.5383596569286055
2214	1	0.6291912880562585
2214	2	0.7546908412568811
2214	3	0.6194487319424624
2214	4	0.6216038814370037
2214	5	1.327151446918574
2214	6	0.8903742077006847
2214	7	1.2090178761454844
2214	8	-2.3497731008377905
2214	9	-0.8537865278205992
2214	10	-0.4344980025017904
2214	11	0.12237161862196899
2214	12	-0.8453040849686744
2214	13	-0.06444455586981945
2214	14	0.11968128512643972
2214	15	-1.1374676360172629
2214	16	0.021324882825922487
2214	17	0.43043758385000075
2214	18	0.46668517582605773
2214	19	1.033773026958059
2214	20	-0.7141762956177047
2214	21	-0.09252344160215387
2214	22	0.2764922500048783
2214	23	-0.17701214977574864
2214	24	-1.309497731733103
2214	25	-0.4650713304624219
2215	1	0.9920337050091277
2215	2	0.42951610053009404
2215	3	0.8684548714162368
2215	4	0.9315627744961645
2215	5	1.4255859052585542
2215	6	2.0103934328034505
2215	7	1.445027749539971
2215	8	0.17818541544799515
2215	9	0.5563892514481736
2215	10	0.44962486426052095
2215	11	0.3358113179761375
2215	12	0.15640347126958432
2215	13	0.36185519149880463
2215	14	0.6838100450107021
2215	15	0.736074178581255
2215	16	0.8096965939083702
2215	17	0.4970395227837301
2215	18	-0.1983681279450522
2215	19	1.0638931596982104
2215	20	-2.8753749179796912
2215	21	-1.3430197680617866
2215	22	-0.27713757498872127
2215	23	-0.013110903042324651
2215	24	-0.13097477995526674
2215	25	0.24081116455015916
2216	1	0.0748978768990014
2216	2	-0.4343763941230705
2216	3	0.573626155521616
2216	4	0.14796673547973072
2216	5	0.694757238814411
2216	6	-0.8794914712008008
2216	7	-8.91696708288471
2216	8	-10.640823349649853
2216	9	-5.477926910205454
2216	10	-2.929110192422094
2216	11	-1.8041483798814184
2216	12	-1.4828429033919903
2216	13	-1.1944441817163458
2216	14	-1.27394688114736
2216	15	-1.9781119283059567
2216	16	-1.4070658830662524
2216	17	-1.504923661332775
2216	18	-1.2213044975379574
2216	19	-0.5016967227332643
2216	20	-1.3075313298527504
2216	21	-4.275570547525
2216	22	-2.738144634044957
2216	23	-2.457226913871981
2216	24	-2.3420640504370347
2216	25	-1.9316968402465378
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35373.0
3	4.0
4	285.0
5	1637.0
6	1802.0
7	1429.0
8	1195.0
9	1091.0
10	1005.0
11	993.0
12	1002.0
13	998.0
14	938.0
15	1126.0
16	1112.0
17	1187.0
18	1381.0
19	1763.0
20	2385.0
21	2861.0
22	3611.0
23	4275.0
24	5149.0
25	5893.0
26	6696.0
27	7163.0
28	8361.0
29	9923.0
30	11591.0
31	14406.0
32	18480.0
33	24211.0
34	32836.0
35	45643.0
36	65146.0
37	101223.0
38	117958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.735336350548955	19.8689008107304	12.997558753467365	25.398204085253283
2	13.266042881966566	20.640662711229048	44.36608664661249	21.7272077601919
3	19.268362494806336	25.809704076450764	29.59087761414524	25.331055814597665
4	11.163323803389067	17.75818058659914	40.340457105099475	30.738038504912318
5	12.446521375768164	38.849234095701604	36.83129811692517	11.872946411605069
6	29.521697580630285	38.56712210492913	19.177296224277825	12.733884090162755
7	27.0675245202322	31.425669751042413	23.584930092827253	17.92187563589814
8	22.952951360629783	40.30542032288137	21.510935349738823	15.230692966750025
9	26.056573177458542	15.931291612167678	21.063020815077326	36.94911439529645
10	14.130302415262838	30.303706847242466	35.87836965145672	19.687621086037975
11	32.952688068892485	23.00677782246311	26.050894547118226	17.98963956152617
12	23.058687663267264	25.3318845285358	33.429202886786605	18.180224921410336
13	28.580283467355873	23.378954495058785	26.0667843629917	21.97397767459364
14	19.329303051490324	24.268027886057524	28.443557298690376	27.95911176376178
15	21.851709735976033	33.8966041411473	23.420505025162708	20.83118109771396
16	19.916465116622298	29.039256598573193	31.01656861280069	20.027709672003823
17	20.212524559315938	30.181070186604682	29.529475791201996	20.076929462877384
18	19.82825254807914	28.593829267167827	32.48609509754185	19.09182308721118
19	23.095783659327644	27.521705280480564	27.776117365331327	21.606393694860472
20	22.42080659116443	29.13668069343005	29.34145545042145	19.10105726498407
21	21.433539148930674	27.864246565486813	27.740462042795134	22.961752242787384
22	21.75688065778332	30.50438786635502	28.083820515327556	19.654910960534107
23	20.58750279029696	29.780447443719016	29.05155730854873	20.580492457435287
24	22.786012747548725	28.167470690784313	29.54479628826801	19.501720273398945
25	21.543896544726696	28.77174534654196	29.40432047521538	20.280037633515967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	251.0
1	251.0
2	278.0
3	305.0
4	305.0
5	305.0
6	479.5
7	654.0
8	654.0
9	654.0
10	967.0
11	1280.0
12	1280.0
13	1280.0
14	2084.0
15	2888.0
16	2888.0
17	2888.0
18	5173.5
19	7459.0
20	7459.0
21	7459.0
22	11903.0
23	16347.0
24	16347.0
25	16347.0
26	24363.0
27	32379.0
28	32379.0
29	32379.0
30	42797.0
31	53215.0
32	53215.0
33	53215.0
34	58803.0
35	64391.0
36	64391.0
37	64391.0
38	66794.5
39	69198.0
40	69198.0
41	69198.0
42	70232.0
43	71266.0
44	71266.0
45	71266.0
46	71204.5
47	71143.0
48	71143.0
49	71143.0
50	65044.0
51	58945.0
52	58945.0
53	58945.0
54	49285.5
55	39626.0
56	39626.0
57	39626.0
58	32600.5
59	25575.0
60	25575.0
61	25575.0
62	19999.5
63	14424.0
64	14424.0
65	14424.0
66	10915.5
67	7407.0
68	7407.0
69	7407.0
70	5321.5
71	3236.0
72	3236.0
73	3236.0
74	2266.5
75	1297.0
76	1297.0
77	1297.0
78	926.0
79	555.0
80	555.0
81	555.0
82	372.0
83	189.0
84	189.0
85	189.0
86	128.5
87	68.0
88	68.0
89	68.0
90	45.5
91	23.0
92	23.0
93	23.0
94	14.0
95	5.0
96	5.0
97	5.0
98	5.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18722377575940916
2	0.11048969623634096
3	0.11196535161178459
4	0.06105524115897973
5	0.017154493739532067
6	0.016601122973740712
7	0.2873838843676448
8	0.2766853828956785
9	0.013465355300923023
10	0.029882021352733285
11	0.012911984535131666
12	0.01401872606671438
13	0.01088295839389669
14	0.012727527613201213
15	0.011067415315827141
16	0.015309924520227548
17	0.014203182988644831
18	0.010514044550035784
19	0.018076778349184332
20	0.013465355300923023
21	0.011251872237757594
22	0.012727527613201213
23	0.013834269144783926
24	0.012358613769340308
25	0.011436329159688046
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	542132.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.73285637730065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.32930865635474	78.04133145236258
2	4.106738062618773	6.795243406276658
3	0.6608571962309241	1.6402381050503587
4	0.25838249241651984	0.8550688654201964
5	0.1509015909663169	0.6242259826261232
6	0.08687354398007163	0.43123778622542264
7	0.06974663150470886	0.4039236632955714
8	0.04823427212789489	0.31924472867366105
9	0.03708418117675671	0.27612722116497673
>10	0.2047392373980128	3.2716887974066617
>50	0.022561849451431033	1.3010796991968576
>100	0.021668288364294654	3.707150089440988
>500	0.0017870753290053331	0.9650898732246761
>1k	0.0011169220806283332	1.3683503296352935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1921	0.354341747028399	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1529	0.28203463363166165	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1504	0.27742321058340036	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1359	0.2506769569034848	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1091	0.20124250182612355	No Hit
GTATCAACGCAGAGTACTTTTTTTT	787	0.14516759755926603	No Hit
CTGTAGGACCTGGAATATGGCGAGA	738	0.13612920838467385	No Hit
GTACATGGGAAGCAGTGGTATCAAC	725	0.13373126839957797	No Hit
CTGAAGGACCTGGAATATGGCGAGA	708	0.13059550072676027	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	613	0.1130720931433673	No Hit
CCCATGTACTCTGCGTTGATACCAC	610	0.11251872237757593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGA	25	0.0059033507	19.068356	2
GCCTCGA	35	0.0021949352	16.255354	16
GTGTAGA	85	2.575889E-7	13.458767	1
GGGCTCG	50	0.001518553	13.275206	16
TAGGACC	250	0.0	12.952066	4
GTATTAC	60	3.9655456E-4	12.711057	1
TAGCCCT	85	3.8224443E-6	12.324631	4
TCCAACG	110	3.8928192E-8	12.068369	18
TAGGACT	95	1.0050444E-6	12.029783	4
GTCCTAT	80	2.757708E-5	11.9166155	1
GGACCTG	515	0.0	11.829646	6
GTACTAC	65	7.776882E-4	11.733283	1
CTCCTAC	65	7.776882E-4	11.733283	1
TAAAGGT	65	7.8437215E-4	11.721326	4
GGCTCGG	65	8.134985E-4	11.670509	17
GTGTAAG	90	7.1511913E-6	11.651803	1
AGGACCT	585	0.0	11.553179	5
GTACAAG	75	2.0028766E-4	11.439951	1
ATGTAGG	85	5.1218667E-5	11.215639	1
GTCCTAC	980	0.0	11.187027	1
>>END_MODULE