FastQCFastQC Report
Thu 26 May 2016
SRR1926870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473064
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5700.12049109634214399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA508.407494E-515.25971
TTGGACA456.647966E-414.8074874
ACCGCTT400.00523884814.2635148
TCTAACA500.001461438613.3423092
CTAGGAC500.001462609413.3408923
GTCTTAG801.9052295E-613.1138051
TTACACA801.9465933E-613.0887594
GTGTAGG905.128477E-712.7164171
TGTAAGG603.9746764E-412.7069612
GTTCTAG701.0486953E-412.26225951
GCAGGAC550.002993361612.1280833
TACATTG550.003022067712.1126455
GGACGTG550.003024470512.1113616
CTGGGCG550.0030973212.0729459
TAAGGAC657.8016636E-411.7282573
CTAGCAC657.8016636E-411.7282573
CAAGGCT657.8714895E-411.7158144
TAGACTG657.8855205E-411.7133275
TAGGACA1104.812937E-711.2498434
ACTTACA600.00572830511.118592
Produced by FastQC (version 0.11.5)