##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926870_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 473064 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.844619332690716 31.0 28.0 31.0 16.0 34.0 2 27.828287504439146 31.0 28.0 31.0 16.0 34.0 3 27.49947575803697 31.0 27.0 31.0 12.0 34.0 4 31.28517917237414 35.0 32.0 37.0 17.0 37.0 5 31.525114995011247 35.0 32.0 37.0 17.0 37.0 6 30.97505411529941 35.0 32.0 37.0 11.0 37.0 7 30.753819779141935 35.0 31.0 37.0 11.0 37.0 8 30.64006772867942 35.0 31.0 37.0 10.0 37.0 9 32.658183670708404 37.0 32.0 39.0 17.0 39.0 10 32.85051494089595 37.0 33.0 39.0 16.0 39.0 11 33.029359663808705 37.0 33.0 39.0 17.0 39.0 12 32.9041461620415 37.0 33.0 39.0 13.0 39.0 13 32.99641697529298 38.0 33.0 39.0 15.0 39.0 14 33.969016877209 39.0 33.0 40.0 15.0 41.0 15 33.54055053861634 39.0 33.0 40.0 10.0 41.0 16 33.61182419292104 39.0 33.0 40.0 10.0 41.0 17 33.83416408773443 39.0 33.0 40.0 10.0 41.0 18 33.865589011212016 39.0 33.0 41.0 10.0 41.0 19 33.23373370199381 39.0 32.0 40.0 8.0 41.0 20 33.529387144234185 39.0 33.0 40.0 2.0 41.0 21 33.50419816346203 39.0 33.0 40.0 2.0 41.0 22 33.516528418987704 39.0 33.0 40.0 2.0 41.0 23 33.49251264099572 39.0 34.0 41.0 2.0 41.0 24 32.17673295790844 38.0 31.0 40.0 2.0 41.0 25 32.724413609997804 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.20172701204818821 1101 2 -0.37033620097900766 1101 3 -1.604384344589807 1101 4 -0.7266898825480332 1101 5 -0.3468993681370556 1101 6 -2.0395384288258285 1101 7 -0.7250470092238572 1101 8 0.028265441673553937 1101 9 -0.03845163827121212 1101 10 -8.827664367363742 1101 11 -3.7647841297425977 1101 12 -1.3592498556777066 1101 13 -0.3699417922177517 1101 14 -0.4523145717723338 1101 15 -1.724383088279037 1101 16 -0.13069422603033587 1101 17 -0.20436929827195627 1101 18 0.08897834872146859 1101 19 -1.0716920180868073 1101 20 -0.05478708656222864 1101 21 -0.056007444772916415 1101 22 -0.05030148821192171 1101 23 0.2070196269641258 1101 24 0.18475789132768483 1101 25 0.09328808837782532 1102 1 -0.3891586749414948 1102 2 -0.6440768675020578 1102 3 -1.0880606675352205 1102 4 -0.4868911645419942 1102 5 -0.12817794778204572 1102 6 -1.0417719598371562 1102 7 -0.34207468549802655 1102 8 0.2749476624415301 1102 9 0.13177838087333527 1102 10 0.07707421398841063 1102 11 -0.27122864915745026 1102 12 -0.07601007728300146 1102 13 -0.2385028617112539 1102 14 -0.4659657344685044 1102 15 0.052960899884674006 1102 16 -0.017788115626025558 1102 17 -0.23820963027343822 1102 18 -0.29063250067066093 1102 19 0.1324982405475481 1102 20 -0.09191166434560927 1102 21 -0.13332541910864393 1102 22 -0.021913898433730594 1102 23 -0.4025798820248738 1102 24 -2.841776213207254 1102 25 -1.4445559343354972 1103 1 -0.26901917696713795 1103 2 -0.7849781747734923 1103 3 -0.4378873392137912 1103 4 -0.47789897475792387 1103 5 -0.6243814310548181 1103 6 0.18408946103741997 1103 7 0.15754942175670195 1103 8 0.19191295501138228 1103 9 -0.3405141472142077 1103 10 -0.39704765879277915 1103 11 -0.6593713213372112 1103 12 -0.663810269405964 1103 13 -0.4294438465502495 1103 14 -0.4772425168266068 1103 15 -0.2808026218126898 1103 16 -0.02549809349012122 1103 17 -0.4966609521629266 1103 18 -0.6824756280983024 1103 19 -2.040633781337764 1103 20 -1.0516493747411317 1103 21 -0.5686175713034416 1103 22 -0.4173238303553788 1103 23 -0.5163793223623401 1103 24 1.050083620564621 1103 25 0.17174110446498503 1104 1 -0.49199905393513177 1104 2 -0.32637114735195283 1104 3 -1.4987151986653622 1104 4 -0.42041800301358023 1104 5 -0.4855950688402437 1104 6 -1.4589821686153783 1104 7 -0.48083030924111014 1104 8 -0.1868171044193332 1104 9 -0.15423534416968465 1104 10 0.061298707592371215 1104 11 0.1405765464555131 1104 12 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23 0.3142128568653959 1105 24 -5.205933222917729 1105 25 -2.6076992176870526 1106 1 -0.24872932189467534 1106 2 -0.9661375950633477 1106 3 -0.7929598029819083 1106 4 -0.9344207138883576 1106 5 -0.3272799817794585 1106 6 0.14785757697944746 1106 7 -2.6250592738954737 1106 8 -0.3254783493364428 1106 9 -0.6071808138808734 1106 10 -0.08690273125655068 1106 11 -0.4724147995980772 1106 12 -0.04931751578277499 1106 13 -0.35408152770966694 1106 14 -0.6960830965367464 1106 15 0.23803795789745408 1106 16 0.20493668911857554 1106 17 -0.37057399564118754 1106 18 -0.47667852664902455 1106 19 0.11298940706803506 1106 20 0.02806077018640707 1106 21 -0.27151612202808195 1106 22 -0.8622513665872589 1106 23 -0.607683670188429 1106 24 -3.3832497127687127 1106 25 -1.6717371564045784 1107 1 0.3504650753201233 1107 2 0.17536511726763848 1107 3 0.5140234372415549 1107 4 0.2454756507784701 1107 5 0.033962022916519174 1107 6 0.06975863762231072 1107 7 -1.7129873768830848 1107 8 -1.6068725684280132 1107 9 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1108 21 0.5952604896660745 1108 22 0.5769890132228355 1108 23 1.0129959899814907 1108 24 0.4386093576960519 1108 25 0.8648945277533429 1109 1 0.023264410968621974 1109 2 -0.008871633449558658 1109 3 -1.1794462894683946 1109 4 0.0700642058139529 1109 5 0.29124724109558997 1109 6 0.37415996632531545 1109 7 0.5943884346826707 1109 8 0.9241103698174946 1109 9 0.5319692057039305 1109 10 0.4603945660407476 1109 11 0.1604093288782238 1109 12 -0.02912558472127813 1109 13 -0.049831625633679266 1109 14 0.13198192500653505 1109 15 -1.2809397154280475 1109 16 -0.0648439610968552 1109 17 -0.14995119893143283 1109 18 0.06582049411086643 1109 19 -1.422498254506582 1109 20 -0.2317512157125421 1109 21 -0.22110484118201867 1109 22 0.11602908778014864 1109 23 0.2264722248370319 1109 24 -0.6781380793962093 1109 25 -0.3612005328244763 1110 1 0.5534445911488035 1110 2 0.3977374346349194 1110 3 0.7338161533022465 1110 4 0.6176938026238759 1110 5 0.4901469604871167 1110 6 0.539482666074246 1110 7 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-3.6650801892901193 2216 11 -2.7069054679329767 2216 12 -2.3081747777992483 2216 13 -2.1902717060018198 2216 14 -2.1849893628280626 2216 15 -3.4897380821400823 2216 16 -2.6122773657245055 2216 17 -2.588809841746908 2216 18 -2.330187024198814 2216 19 -1.7102615573778017 2216 20 -2.5311614172810124 2216 21 -4.908738518024329 2216 22 -3.4390004021414278 2216 23 -3.4418406923967453 2216 24 -3.789516004083488 2216 25 -3.0723418900089996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30917.0 3 0.0 4 235.0 5 1382.0 6 1730.0 7 1369.0 8 1131.0 9 1042.0 10 963.0 11 932.0 12 942.0 13 916.0 14 911.0 15 1025.0 16 1026.0 17 1135.0 18 1307.0 19 1592.0 20 2070.0 21 2684.0 22 3201.0 23 3904.0 24 4615.0 25 5376.0 26 5997.0 27 6597.0 28 7280.0 29 8570.0 30 10217.0 31 12215.0 32 15884.0 33 20739.0 34 27520.0 35 37974.0 36 54678.0 37 85049.0 38 109939.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.2253790567065 17.21130359068443 11.647850652610973 25.915466699998092 2 15.7895516638516 18.634672288330393 40.4173492670248 25.158426780793214 3 20.102277754376754 22.166247917720593 28.343553347627946 29.3879209802747 4 12.164494838382128 15.605855474699611 36.921433406667795 35.30821628025046 5 13.319082445824426 37.35910333584944 35.79467727237167 13.52713694595446 6 34.5792614080862 35.593736388739714 17.00059625248967 12.826405950684421 7 30.26941295709663 30.48060082524416 20.649975191786574 18.600011025872636 8 26.079185501174596 36.88651802600222 19.138474977313784 17.895821495509402 9 26.10308031570646 15.727602749428613 18.891855948884814 39.27746098598011 10 14.868091315703214 29.003203874255867 33.65802078817421 22.470684021866706 11 36.30695524767379 22.19920039071337 22.249942386692066 19.243901974920767 12 23.328977914219386 24.32924431364092 30.594282138130215 21.747495634009482 13 29.468351861170195 20.79867732810128 24.90137045384391 24.831600356884618 14 21.40375877692807 20.76418738490358 26.771718470187515 31.060335367980834 15 24.28903364391532 29.128319622481147 22.462540936989278 24.120105796614254 16 23.39015231603251 27.917346774807385 25.635989208480986 23.05651170067912 17 22.834102941425215 27.880956005480286 26.205155528492412 23.079785524602087 18 22.495052686771647 27.894812509514068 26.88716743061076 22.72296737310353 19 24.22126086809432 26.87590919855205 26.058053384755915 22.84477654859772 20 23.71732121148052 26.846239230403295 26.902690417041068 22.53374914107511 21 23.92813272358626 26.355492692126354 26.3529556030555 23.363418981231884 22 23.520432326375968 26.499653259362 26.721017556916205 23.258896857345825 23 23.484688311358813 26.633923361213775 26.422494354845533 23.458893972581887 24 23.817708994037805 26.54530846970274 26.492029261279548 23.144953274979915 25 23.56797784284914 26.367643794200664 26.658350687653943 23.406027675296258 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 119.0 1 119.0 2 143.5 3 168.0 4 168.0 5 168.0 6 255.0 7 342.0 8 342.0 9 342.0 10 565.0 11 788.0 12 788.0 13 788.0 14 1291.0 15 1794.0 16 1794.0 17 1794.0 18 3022.5 19 4251.0 20 4251.0 21 4251.0 22 6554.5 23 8858.0 24 8858.0 25 8858.0 26 12400.5 27 15943.0 28 15943.0 29 15943.0 30 21020.5 31 26098.0 32 26098.0 33 26098.0 34 31207.0 35 36316.0 36 36316.0 37 36316.0 38 41584.0 39 46852.0 40 46852.0 41 46852.0 42 51459.0 43 56066.0 44 56066.0 45 56066.0 46 59730.5 47 63395.0 48 63395.0 49 63395.0 50 63424.0 51 63453.0 52 63453.0 53 63453.0 54 58878.5 55 54304.0 56 54304.0 57 54304.0 58 47484.0 59 40664.0 60 40664.0 61 40664.0 62 33825.0 63 26986.0 64 26986.0 65 26986.0 66 21214.5 67 15443.0 68 15443.0 69 15443.0 70 11253.5 71 7064.0 72 7064.0 73 7064.0 74 4914.5 75 2765.0 76 2765.0 77 2765.0 78 1878.0 79 991.0 80 991.0 81 991.0 82 643.5 83 296.0 84 296.0 85 296.0 86 189.0 87 82.0 88 82.0 89 82.0 90 49.5 91 17.0 92 17.0 93 17.0 94 12.0 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21920924018737423 2 0.1281010603216478 3 0.13211743019971928 4 0.07271743358192549 5 0.023464055603470143 6 0.023252667715150593 7 0.3056668865100705 8 0.2984796983072058 9 0.019659073613718228 10 0.04206618977559062 11 0.017545194730522722 12 0.019024909948759577 13 0.01733380684220317 14 0.019236297837079128 15 0.01712241895388362 16 0.021138788831955083 17 0.020293237278676882 18 0.016911031065564068 19 0.0253665465983461 20 0.018813522060440024 21 0.01712241895388362 22 0.018602134172120473 23 0.019447685725398678 24 0.017756582618842272 25 0.016699643177244518 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 473064.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.08990156612052 #Duplication Level Percentage of deduplicated Percentage of total 1 93.4640809793092 83.26705774414575 2 5.023481899450738 8.950830158825086 3 0.8137823298437498 2.1749936298608366 4 0.2719189473330256 0.9690092900744941 5 0.126972269927455 0.5655973514731926 6 0.07218975022735906 0.3858826645102946 7 0.048419138273892465 0.30195593839162205 8 0.034779235142464164 0.24787829083096738 9 0.02409315021176271 0.1931810742705326 >10 0.10865911101361364 1.6291518162947785 >50 0.007353447044263536 0.47485476211163696 >100 0.0040325343212642304 0.7191501563439309 >500 2.37207901250746E-4 0.12045712286686949 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 570 0.12049109634214399 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAA 50 8.407494E-5 15.2597 1 TTGGACA 45 6.647966E-4 14.807487 4 ACCGCTT 40 0.005238848 14.263514 8 TCTAACA 50 0.0014614386 13.342309 2 CTAGGAC 50 0.0014626094 13.340892 3 GTCTTAG 80 1.9052295E-6 13.113805 1 TTACACA 80 1.9465933E-6 13.088759 4 GTGTAGG 90 5.128477E-7 12.716417 1 TGTAAGG 60 3.9746764E-4 12.706961 2 GTTCTAG 70 1.0486953E-4 12.2622595 1 GCAGGAC 55 0.0029933616 12.128083 3 TACATTG 55 0.0030220677 12.112645 5 GGACGTG 55 0.0030244705 12.111361 6 CTGGGCG 55 0.00309732 12.072945 9 TAAGGAC 65 7.8016636E-4 11.728257 3 CTAGCAC 65 7.8016636E-4 11.728257 3 CAAGGCT 65 7.8714895E-4 11.715814 4 TAGACTG 65 7.8855205E-4 11.713327 5 TAGGACA 110 4.812937E-7 11.249843 4 ACTTACA 60 0.005728305 11.11859 2 >>END_MODULE