FastQCFastQC Report
Thu 26 May 2016
SRR1926870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473064
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10060.21265621564946816No Hit
GGTATCAACGCAGAGTACTTTTTTT7170.15156511592511795No Hit
TATCAACGCAGAGTACTTTTTTTTT5090.10759643515465137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG307.6084357E-419.0381724
GTAGGAC855.4569682E-1116.8001693
GTCCTAA653.2476291E-614.6634051
TACACTC653.2908465E-614.6447485
AGTCCCA653.3974302E-614.599853510
TTATTCC400.00520215914.2786284
TAGGACC759.416108E-713.9613264
TTAAGCA500.001474378413.326724
CTACACT1500.013.326724
GTCCTAC1301.2732926E-1113.1970651
TTGCACA550.003017299612.11524
CGGCTCT550.00308750912.0780619
TGGAATA1103.8506187E-812.07678511
TCCTACA1459.094947E-1111.8305962
GGAGTGT657.8715914E-411.7157986
CTAAATT752.0905795E-411.386682519
ACTGTTC855.362254E-511.1645958
TGAGTTA855.3680327E-511.16341412
TGTAACC600.005728399411.1185682
ATAGTGG600.005778402611.10566
Produced by FastQC (version 0.11.5)