FastQCFastQC Report
Thu 26 May 2016
SRR1926869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633739
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8040.12686610734071913No Hit
GTCCTACAGTGTGCATTTCTCATTT7340.11582055073145253No Hit
GTATCAACGCAGAGTACTTTTTTTT7240.11424261407298587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTT250.006089903418.9665411
CCCGTTT307.807838E-418.96653712
TAGGACT1152.3646862E-1114.0731684
TAGGACC1900.014.0295964
GTAGGAC4150.013.5506823
GTCTAGA500.001453177713.353511
ATAGCCC500.001461022413.3439773
ATCCCGT500.001517864513.27657710
GTCCTAC4450.013.0748631
TCCAACG604.147291E-412.64435918
AGGACCT2900.012.4706415
GTCCTAT1154.991307E-912.4411571
GGACCTG2900.012.1424676
TGTAGGA4800.012.1137952
AGGACTG1603.6379788E-1211.8963365
GTATAGC657.746414E-411.7393481
CTGTAGG4800.011.7240621
TCTATAC1156.727714E-811.60345653
GGCGAGA2300.011.54576119
AGAACAG2150.011.5090135
Produced by FastQC (version 0.11.5)