##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926869_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633739
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.83571628067706	31.0	28.0	31.0	16.0	34.0
2	27.80854736729158	31.0	28.0	31.0	16.0	34.0
3	27.468292151816442	31.0	27.0	31.0	12.0	34.0
4	31.235131497351432	35.0	32.0	37.0	17.0	37.0
5	31.49437071097092	35.0	32.0	37.0	17.0	37.0
6	30.93077276291975	35.0	32.0	37.0	11.0	37.0
7	30.702278067153827	35.0	31.0	37.0	11.0	37.0
8	30.62324237580455	35.0	31.0	37.0	10.0	37.0
9	32.63753374812028	37.0	32.0	39.0	17.0	39.0
10	32.84734567384996	37.0	33.0	39.0	17.0	39.0
11	33.013114231568515	37.0	33.0	39.0	17.0	39.0
12	32.89037127271637	37.0	33.0	39.0	13.0	39.0
13	32.97688480589012	37.0	33.0	39.0	15.0	39.0
14	33.934548765343465	39.0	33.0	40.0	15.0	41.0
15	33.52829950500127	39.0	33.0	40.0	10.0	41.0
16	33.597769744326925	38.0	33.0	40.0	10.0	41.0
17	33.80392243494562	39.0	33.0	40.0	10.0	41.0
18	33.828118515666546	39.0	33.0	40.0	10.0	41.0
19	33.21285260967054	39.0	32.0	40.0	8.0	41.0
20	33.51817388546389	39.0	33.0	40.0	6.0	41.0
21	33.48771339620885	39.0	33.0	40.0	2.0	41.0
22	33.50420599016314	39.0	33.0	40.0	2.0	41.0
23	33.49244405031093	39.0	34.0	40.0	2.0	41.0
24	32.179955786214826	38.0	31.0	40.0	2.0	41.0
25	32.738472778225734	39.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.2590352683001953
1101	2	-0.291788159999399
1101	3	-1.2304836736345912
1101	4	-0.6049451059818942
1101	5	-0.24454230508197128
1101	6	-2.2294075633475288
1101	7	-0.8184735800138121
1101	8	-0.27041884117491577
1101	9	-0.2137438154334319
1101	10	-9.00166769805044
1101	11	-4.102019275494786
1101	12	-1.6747756932201163
1101	13	-0.8637331404365867
1101	14	-0.8705365911126535
1101	15	-2.2144659944507765
1101	16	-0.5366092846377697
1101	17	-0.24627633813169325
1101	18	-0.2042006063604802
1101	19	-1.0217636849689171
1101	20	-0.02813332907825128
1101	21	-0.42212157306182974
1101	22	0.02026021200693151
1101	23	-0.29957162653719394
1101	24	-0.3502664794136727
1101	25	-0.21068047076474983
1102	1	-0.04016329439108901
1102	2	-0.3683015752802312
1102	3	-0.7069167497155853
1102	4	-0.3892935277604188
1102	5	-0.015687104541218133
1102	6	-0.7481108932139122
1102	7	-0.15264368518305815
1102	8	0.552342187257878
1102	9	0.07737198908513676
1102	10	0.05035250282031711
1102	11	-0.21207828953024688
1102	12	-0.04780561900308555
1102	13	-0.28365680681725536
1102	14	-0.5658575723888433
1102	15	0.35841622555118136
1102	16	0.07152545762816231
1102	17	-0.1209119099624445
1102	18	-0.3410788025747493
1102	19	0.11403573558744995
1102	20	-0.2093005006149582
1102	21	-0.22240355682557578
1102	22	-0.4276979660911948
1102	23	-0.8857302975948329
1102	24	-3.3397279336669854
1102	25	-1.6704653528846123
1103	1	-0.02518406900030712
1103	2	-0.3686699356419112
1103	3	-0.07577130914744146
1103	4	-0.10602898553396756
1103	5	-0.17773134439185867
1103	6	0.457466219939068
1103	7	0.18285252596750823
1103	8	0.5290645579427995
1103	9	0.162401457914072
1103	10	0.18495046379700142
1103	11	0.14542116136113492
1103	12	0.01294867319591475
1103	13	0.016229710735920833
1103	14	-0.1486471356644543
1103	15	0.12836030799068965
1103	16	-0.0022198291895705324
1103	17	-0.300465850731932
1103	18	-0.1384188289319681
1103	19	-2.310923996447549
1103	20	-0.7053995415052512
1103	21	-0.6769716829009127
1103	22	-0.3148561459791779
1103	23	-0.7350411662846597
1103	24	1.164646631622027
1103	25	0.38112111341842336
1104	1	-0.33405242938143687
1104	2	-0.39393718649297327
1104	3	-1.4476169836012325
1104	4	-0.6902130036178669
1104	5	-0.347930960739415
1104	6	-1.6880110040743332
1104	7	-0.30114844667248875
1104	8	0.10762550104335844
1104	9	-0.004041561592764253
1104	10	-0.009459332849196755
1104	11	-0.11866748396749216
1104	12	0.13493619098261433
1104	13	-0.19094260865023926
1104	14	-0.28420095507762966
1104	15	0.05503968802274528
1104	16	-0.5124415550852106
1104	17	-0.37108807015231093
1104	18	-0.37215300101719606
1104	19	-1.3398802996950856
1104	20	-0.47525648237233753
1104	21	-0.24180872067177006
1104	22	-0.16545684123665438
1104	23	-0.1194793766270621
1104	24	1.2762210475877325
1104	25	0.09565014852351794
1105	1	0.4888166372143843
1105	2	0.2599494146405732
1105	3	0.6358635778582062
1105	4	0.4434413534490851
1105	5	0.754048316625088
1105	6	0.3065304854757933
1105	7	0.6519909440466023
1105	8	0.8360737387337593
1105	9	-0.05080475677519303
1105	10	0.3471150118195965
1105	11	0.22393457944023254
1105	12	0.3068222607665376
1105	13	0.32948889152687855
1105	14	0.014859220267744888
1105	15	0.6672197712675256
1105	16	0.1477448600759601
1105	17	0.20137030746015938
1105	18	0.34182481513582985
1105	19	-0.2407968778034828
1105	20	0.1553525771388209
1105	21	0.1925374978900436
1105	22	0.4371035997835335
1105	23	0.17853581111647543
1105	24	-4.39190350962091
1105	25	-1.6969862312143462
1106	1	-0.30443037732351996
1106	2	-0.795200507477805
1106	3	-0.6520160950389879
1106	4	-1.005085385907094
1106	5	-0.2985347571028427
1106	6	0.08570688734495135
1106	7	-2.643920270906669
1106	8	-0.4280455499474449
1106	9	-1.0744158678863016
1106	10	-0.3408879736425021
1106	11	-0.7674757268941406
1106	12	-0.5412274536675241
1106	13	-0.702692442844878
1106	14	-1.0318016052774226
1106	15	0.10582633928413543
1106	16	-0.3645409654692102
1106	17	-0.8101042512106602
1106	18	-0.788493850492415
1106	19	0.24210810662019355
1106	20	-0.2487459431104
1106	21	-0.47215292267070197
1106	22	-0.8244131447647547
1106	23	-1.055092823359125
1106	24	-3.9997143870871597
1106	25	-2.471791527164502
1107	1	-0.45623223109837596
1107	2	-0.5512002767174486
1107	3	-0.0975854962158671
1107	4	-0.5243827206249883
1107	5	-0.49137349407449804
1107	6	-0.6313424569110389
1107	7	-2.6760440353772665
1107	8	-2.409180890895872
1107	9	-1.3635105180920633
1107	10	-0.8794153379746383
1107	11	-1.0259753999836363
1107	12	-0.6537615869700915
1107	13	-0.9297317874854656
1107	14	-0.887134092489454
1107	15	-2.1974793028065527
1107	16	-1.2599969094713614
1107	17	-1.13422640035877
1107	18	-1.2785326951522364
1107	19	-0.4093668366512162
1107	20	-0.37611604426035683
1107	21	-0.6391368848260015
1107	22	-0.9830224290230518
1107	23	-1.187655032504928
1107	24	-4.79960669892132
1107	25	-2.4928195645476805
1108	1	0.45210320184023445
1108	2	0.20818495885826138
1108	3	0.9424749724160293
1108	4	0.7956097207960227
1108	5	0.8323646431556995
1108	6	-2.583626827449379
1108	7	-0.9147607566336688
1108	8	0.5248705074412428
1108	9	0.4067632704356896
1108	10	0.8689049778059967
1108	11	0.5618384910048562
1108	12	0.8512312743719761
1108	13	0.8421377010506887
1108	14	0.7377546284310128
1108	15	-0.10639842601138838
1108	16	0.8431105063344688
1108	17	0.6336832326302257
1108	18	0.49558757023787336
1108	19	0.52170312219652
1108	20	-0.9602724228611805
1108	21	-0.03193103952492038
1108	22	0.3412490079468
1108	23	0.5527917634974244
1108	24	-0.32300470009710125
1108	25	0.2306498232074219
1109	1	0.618864389073746
1109	2	0.47906917461352805
1109	3	-0.6491030380985201
1109	4	0.5603404406701813
1109	5	1.0800878703776249
1109	6	1.2845625172540203
1109	7	1.1638148115246274
1109	8	1.4875210849136131
1109	9	1.1053226942051992
1109	10	1.0877442376478612
1109	11	0.9142891264314414
1109	12	0.7796184913277244
1109	13	1.0043515521083535
1109	14	0.976896492073017
1109	15	-0.505589136778454
1109	16	0.708389721468798
1109	17	0.6015393405906551
1109	18	0.9206217218295407
1109	19	-0.6471609751665639
1109	20	0.5725876177067661
1109	21	0.5818144586743585
1109	22	0.7934740358890124
1109	23	0.7107894453287784
1109	24	-0.15571985512463016
1109	25	0.42959422855576435
1110	1	0.7034534700441917
1110	2	0.865988563683878
1110	3	1.3614331901442114
1110	4	1.0856933675477762
1110	5	1.00680511420817
1110	6	1.329506945697343
1110	7	1.320080067913672
1110	8	1.4787568958334596
1110	9	1.1545200166387062
1110	10	1.296746809402677
1110	11	0.908718693236807
1110	12	0.8794399596587823
1110	13	0.936843498777634
1110	14	0.8182045123120574
1110	15	1.0712070572695396
1110	16	1.0554550816268176
1110	17	0.678157820048277
1110	18	0.7391403992244534
1110	19	-1.6794549845507092
1110	20	0.26450099103610114
1110	21	0.4694099802666827
1110	22	0.7782447125730627
1110	23	0.5352043660006629
1110	24	-0.31289104738526063
1110	25	-0.015729937155938956
1111	1	0.6016230097634008
1111	2	0.36406934651162715
1111	3	1.0523443438934343
1111	4	1.1626026196504782
1111	5	1.1906011581006624
1111	6	1.382939799201285
1111	7	1.35707984401337
1111	8	1.666277917863038
1111	9	1.0489916872261347
1111	10	0.9255411916135472
1111	11	0.7409470835944347
1111	12	0.8695040071939175
1111	13	0.8158309071466903
1111	14	0.9071230537672506
1111	15	-0.04854647167032766
1111	16	-0.0141839368665444
1111	17	0.44492298276757225
1111	18	0.6428000145376274
1111	19	1.185461846025035
1111	20	0.6012430307779013
1111	21	0.768753641127006
1111	22	0.898005269773563
1111	23	0.7593506582417646
1111	24	1.0325429426888277
1111	25	0.8334516218929977
1112	1	-0.27861890271448786
1112	2	-5.632348906239315E-4
1112	3	-1.7136989423487101
1112	4	-0.3894454069776856
1112	5	-0.4254739918133197
1112	6	0.8269404838592394
1112	7	0.5571517911206421
1112	8	1.0796469281938315
1112	9	-0.11978713634195515
1112	10	0.4587127728929872
1112	11	0.10537755551523986
1112	12	0.290429397850275
1112	13	0.03710791027889826
1112	14	0.01927544238219525
1112	15	0.6151172413612827
1112	16	0.4647449409036355
1112	17	-0.4923089683238686
1112	18	-0.08180212472837667
1112	19	0.8008010853391028
1112	20	0.49113397869071207
1112	21	0.3509991451580703
1112	22	0.1323236531298022
1112	23	0.3657680694417209
1112	24	1.6556706497712668
1112	25	0.8362299019896611
1113	1	-0.4810776567100845
1113	2	0.3111758103712816
1113	3	-4.2766312907789015
1113	4	-1.1914416514770245
1113	5	0.002632060467455233
1113	6	0.6799551570685658
1113	7	1.1695812560334673
1113	8	1.3575587551541197
1113	9	0.8330827637502622
1113	10	0.7835425569755898
1113	11	0.7792558027571488
1113	12	0.5744895431967478
1113	13	0.128344597602414
1113	14	-0.2105564201263448
1113	15	0.6215044592806578
1113	16	0.9952920686145532
1113	17	0.6595804880841314
1113	18	0.5712737067614455
1113	19	1.4426390827876503
1113	20	0.569776838222559
1113	21	0.7733821202217399
1113	22	0.7316079101283606
1113	23	0.5535306797535853
1113	24	1.9858622949767906
1113	25	1.277870090624731
1114	1	0.7943070466276652
1114	2	0.6171081025838383
1114	3	0.7542126268588518
1114	4	0.6256101155380591
1114	5	0.8964806183658993
1114	6	1.392497200498358
1114	7	1.684155676477296
1114	8	1.397753703272766
1114	9	0.5431426964807713
1114	10	0.6193687996983854
1114	11	0.3719419134183113
1114	12	-0.05640106289561686
1114	13	0.400634523377299
1114	14	0.6218406032464685
1114	15	-1.6493562280877327
1114	16	0.09986848999214715
1114	17	0.17019687096757252
1114	18	0.609468673936604
1114	19	1.1459777837052272
1114	20	1.0876966293631867
1114	21	0.9974476365102944
1114	22	1.009570959551425
1114	23	0.2830349245916537
1114	24	1.5149892208755418
1114	25	1.0811649615645038
1115	1	-3.4255263115035675
1115	2	-1.1438166110004495
1115	3	0.020398834268466004
1115	4	0.6006528919106238
1115	5	0.7117406243174003
1115	6	1.4417067675270765
1115	7	1.2128883799440402
1115	8	1.6169230977081206
1115	9	0.9450285765581441
1115	10	-0.18902280869322396
1115	11	0.4571877845684398
1115	12	-0.08059761102833818
1115	13	0.4185113274243193
1115	14	0.5684649894985156
1115	15	0.8493511815239358
1115	16	0.8379692190503221
1115	17	0.6952805638704191
1115	18	0.6329305843665978
1115	19	-1.4244507239573245
1115	20	0.07241988483112749
1115	21	0.25896377994133246
1115	22	0.5411901382450708
1115	23	0.4882313239369864
1115	24	-0.5216310737234728
1115	25	0.38410351237539686
1116	1	0.43438732386201906
1116	2	-0.4225602983311987
1116	3	0.1430759549593823
1116	4	0.16886357038533717
1116	5	0.6738033118677151
1116	6	1.0050923709816608
1116	7	1.1617950988198338
1116	8	-1.6851966783293548
1116	9	-0.4196954957539418
1116	10	-0.6287066263028223
1116	11	0.07637372469871906
1116	12	0.24271169146745564
1116	13	0.09062213764169513
1116	14	0.21941737916403525
1116	15	-0.40876837494244
1116	16	0.06333517089105811
1116	17	-0.02271786253666619
1116	18	0.050605143394633956
1116	19	0.47389151420413356
1116	20	0.09434222985051832
1116	21	0.12916432155004998
1116	22	0.11439605934585728
1116	23	0.19452752535688234
1116	24	1.2623018107089337
1116	25	0.4684078379261507
1201	1	0.1704617521569105
1201	2	0.08663763303137983
1201	3	0.2818046698122316
1201	4	0.3557258362077036
1201	5	0.17409142007336698
1201	6	0.5877445659355622
1201	7	0.14365761071326943
1201	8	0.7211671295383582
1201	9	0.2601147834546964
1201	10	0.4428405865322418
1201	11	0.4693727978310349
1201	12	0.5742432377780275
1201	13	0.6576857306073407
1201	14	0.4106566340608495
1201	15	1.025660863221546
1201	16	0.5613942853633205
1201	17	0.19009157182797765
1201	18	0.23977740134272807
1201	19	-0.26708998125175754
1201	20	0.5302448185181277
1201	21	0.64767399214292
1201	22	0.6863492894387093
1201	23	0.7034998915762429
1201	24	-0.4115514981266557
1201	25	0.2808873320040419
1202	1	0.20388203635138424
1202	2	0.07570251604294853
1202	3	-0.21795586658623733
1202	4	0.025149821625998214
1202	5	0.18124626700265267
1202	6	0.9895772761985526
1202	7	0.3829375459883515
1202	8	1.1141919965546876
1202	9	0.8932216725667743
1202	10	0.7667650927257483
1202	11	0.5659080152546849
1202	12	0.7473730967967427
1202	13	0.5699385948710045
1202	14	0.6624084381749711
1202	15	1.240942128333323
1202	16	1.007123231165501
1202	17	0.6776917745583262
1202	18	0.5774943135177111
1202	19	0.12337518260644487
1202	20	0.653319810148524
1202	21	0.7352188625070895
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2211	3	0.37268740826756286
2211	4	0.47415529107091814
2211	5	0.36708437900325563
2211	6	-2.009618393666507
2211	7	0.15182037874445697
2211	8	0.6122616042308344
2211	9	0.5308472900084311
2211	10	0.74766934905157
2211	11	0.6059825813895543
2211	12	0.3440725044312529
2211	13	0.26438777066918107
2211	14	0.4987225243613125
2211	15	1.0437487673688892
2211	16	0.09385840783424726
2211	17	0.3845087090196131
2211	18	0.3877007897946996
2211	19	0.5572781709294645
2211	20	0.5245228987762474
2211	21	0.09201971036568324
2211	22	-2.6179585347193886
2211	23	-1.2470550758230985
2211	24	1.047588449443417
2211	25	0.4813519754133324
2212	1	-0.6786908684655906
2212	2	-0.37826159639480395
2212	3	-3.3184643594997922
2212	4	-1.9169590847203395
2212	5	-0.7583543123914644
2212	6	-0.058208032862410164
2212	7	0.11402031762660769
2212	8	-0.0977920921652995
2212	9	-0.03698213425003871
2212	10	-0.10099943965719405
2212	11	-0.257332663338083
2212	12	-0.5510211748063298
2212	13	-0.7387914963342013
2212	14	-0.4462258576290381
2212	15	-1.3044341154575676
2212	16	-0.4871768374248475
2212	17	-0.29046675842754155
2212	18	-0.13441362205988128
2212	19	0.6061724503367358
2212	20	-0.6586171161425867
2212	21	-0.6320294040898276
2212	22	-0.3510566971979614
2212	23	-0.04670051637459949
2212	24	0.8672442914177125
2212	25	0.44121159416702227
2213	1	-0.6829572300995324
2213	2	-0.4522370740325634
2213	3	0.28118113449251325
2213	4	-6.676036801890721
2213	5	-1.6798109066758826
2213	6	-3.3403040614497748
2213	7	-1.2571919038498365
2213	8	-3.099292361589864
2213	9	-0.9707481224709866
2213	10	-0.2798368490218266
2213	11	-0.05955045292222394
2213	12	-0.17538849975126425
2213	13	-0.2609136165637338
2213	14	-0.2622949059458435
2213	15	-2.673081604137007
2213	16	-0.8526394441311567
2213	17	-0.3725779126045623
2213	18	-0.2325380489418407
2213	19	0.3794701124877804
2213	20	-1.2466801898514746
2213	21	-0.28700335971512914
2213	22	-0.3748199439057842
2213	23	-0.18098077464404128
2213	24	-0.6522696099445326
2213	25	-0.4224312150331144
2214	1	0.03914740340711731
2214	2	0.20453373533567643
2214	3	0.04892143725789211
2214	4	0.23950177998936795
2214	5	0.13017390904215276
2214	6	0.12294537489127322
2214	7	0.7283140088175344
2214	8	-3.1057755898602366
2214	9	-1.270729717280723
2214	10	-0.909110304136167
2214	11	-0.46806285341916976
2214	12	-1.8005483948416803
2214	13	-0.9846056977306219
2214	14	-0.6637397133815455
2214	15	-2.266138246110046
2214	16	-0.8738882362905471
2214	17	-0.8389930416709603
2214	18	-0.6219044502670101
2214	19	0.21340928333395937
2214	20	-1.6152487261787165
2214	21	-0.914951641130493
2214	22	-0.5742263765197819
2214	23	-0.7985623326433995
2214	24	-1.8492741652291258
2214	25	-1.2205446830310933
2215	1	0.3613672073487173
2215	2	-0.08203844071712751
2215	3	0.23929707520278853
2215	4	0.7209659551360588
2215	5	0.4281462547694197
2215	6	0.909815591692599
2215	7	0.44455967256893913
2215	8	-0.9840186152181296
2215	9	-0.4153509923265801
2215	10	-0.6533584730288879
2215	11	-0.5539190209971281
2215	12	-0.7793666255190033
2215	13	-0.3887231906349413
2215	14	-0.1207165763583049
2215	15	-0.46696973982276546
2215	16	-0.09419320615334215
2215	17	-0.44228792434240205
2215	18	-1.3308292382849771
2215	19	-0.07740653103684281
2215	20	-4.111350211052372
2215	21	-2.496111557092771
2215	22	-1.2960881762239609
2215	23	-1.3376554181868698
2215	24	-1.4179705589804854
2215	25	-1.213297540180296
2216	1	-0.2961491588055196
2216	2	-0.6972178739166388
2216	3	0.25198609029601826
2216	4	-0.09489819878972128
2216	5	-0.5228173550167021
2216	6	-1.6807674000963466
2216	7	-9.501864777346434
2216	8	-10.971093549823454
2216	9	-5.6663520204567845
2216	10	-3.218572177235128
2216	11	-2.2585125598632487
2216	12	-2.087845027790678
2216	13	-1.6499731731497356
2216	14	-1.5901314513740417
2216	15	-2.658542044652876
2216	16	-1.6395996672872215
2216	17	-1.8050351651766547
2216	18	-1.6251061550134196
2216	19	-0.9148640419825824
2216	20	-1.7850858556970017
2216	21	-3.7648163578313465
2216	22	-2.6590251315597477
2216	23	-2.40363147744074
2216	24	-3.01660811161096
2216	25	-2.3995359824581293
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41384.0
3	2.0
4	275.0
5	1862.0
6	2376.0
7	1824.0
8	1480.0
9	1353.0
10	1340.0
11	1255.0
12	1223.0
13	1279.0
14	1236.0
15	1262.0
16	1390.0
17	1520.0
18	1798.0
19	2144.0
20	2695.0
21	3566.0
22	4610.0
23	5384.0
24	6140.0
25	7051.0
26	8004.0
27	8763.0
28	9714.0
29	11348.0
30	13976.0
31	17007.0
32	21492.0
33	27855.0
34	37613.0
35	51421.0
36	73366.0
37	114288.0
38	144443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70446512584111	18.28384821576829	12.356071448339119	25.655615210051476
2	14.872204826167598	19.49899362682988	41.649788456252	23.97901309075053
3	19.296080841530504	23.688584420057445	28.81259262302075	28.2027421153913
4	11.757906870253114	16.687088471680536	38.20893401335839	33.346070644707964
5	12.784195382796678	37.80838306341512	36.323188449257955	13.084233104530238
6	32.67133741273917	36.845020983634505	17.96827280776655	12.515368795859777
7	28.941591687834833	30.989562313542084	21.700733565985328	18.36811243263775
8	24.864018838751743	37.93023774745248	20.22206431825843	16.983679095537347
9	25.882676018326645	15.833008476783148	19.90568375333208	38.37863175155813
10	14.434957212742884	29.46503694653211	34.512844070006615	21.587161770718392
11	34.79781448362283	22.881163336100478	23.590098891143988	18.730923289132704
12	22.90054891117361	25.026908957763194	31.633097544577332	20.439444586485862
13	28.97509102358328	21.94855964155568	25.36949972144495	23.706849613416097
14	20.718858109643694	21.959199262644884	27.524557460670184	29.797385167041234
15	23.2955773944141	30.62476425553674	23.060582578819535	23.019075771229627
16	22.21429586022948	28.53711273496315	27.383721847824372	21.864869556983002
17	21.85973545533027	28.676156100609674	27.41656237127476	22.047546072785295
18	21.679652668508957	28.13845913473299	28.90120590690796	21.280682289850088
19	23.73638688089902	27.153319233561668	26.947504656081318	22.162789229458
20	23.2133556811833	27.58980963293341	27.886044904027347	21.310789781855945
21	22.990109354212006	26.983139976232152	26.975722386443802	23.05102828311204
22	22.796944540892017	27.93046305356523	27.251191565922795	22.02140083961996
23	22.531561262545196	27.717724969184466	27.254979017027853	22.495734751242484
24	23.09930952850661	27.304478200828562	27.677096074176365	21.91911619648846
25	22.879900761648038	27.30317253395536	27.578413764586678	22.238512939809922
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	191.0
1	191.0
2	247.0
3	303.0
4	303.0
5	303.0
6	432.5
7	562.0
8	562.0
9	562.0
10	878.5
11	1195.0
12	1195.0
13	1195.0
14	2033.5
15	2872.0
16	2872.0
17	2872.0
18	4916.5
19	6961.0
20	6961.0
21	6961.0
22	10873.0
23	14785.0
24	14785.0
25	14785.0
26	21158.5
27	27532.0
28	27532.0
29	27532.0
30	35942.5
31	44353.0
32	44353.0
33	44353.0
34	51539.0
35	58725.0
36	58725.0
37	58725.0
38	64834.0
39	70943.0
40	70943.0
41	70943.0
42	75068.5
43	79194.0
44	79194.0
45	79194.0
46	81788.0
47	84382.0
48	84382.0
49	84382.0
50	81637.0
51	78892.0
52	78892.0
53	78892.0
54	70911.5
55	62931.0
56	62931.0
57	62931.0
58	53709.0
59	44487.0
60	44487.0
61	44487.0
62	36455.5
63	28424.0
64	28424.0
65	28424.0
66	21869.0
67	15314.0
68	15314.0
69	15314.0
70	11245.0
71	7176.0
72	7176.0
73	7176.0
74	5026.0
75	2876.0
76	2876.0
77	2876.0
78	2009.5
79	1143.0
80	1143.0
81	1143.0
82	748.5
83	354.0
84	354.0
85	354.0
86	234.0
87	114.0
88	114.0
89	114.0
90	69.0
91	24.0
92	24.0
93	24.0
94	14.5
95	5.0
96	5.0
97	5.0
98	3.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2199643701902518
2	0.12197450369947249
3	0.12576155167979247
4	0.06769348264821953
5	0.023984637208693167
6	0.02366904987699984
7	0.2988612031135846
8	0.29191828181633134
9	0.019882001896679866
10	0.04039517845674639
11	0.01783068424067321
12	0.019093033567446533
13	0.017672890574826544
14	0.019408620899139868
15	0.017041715911439884
16	0.02035538289421986
17	0.019250827233293202
18	0.017041715911439884
19	0.025089192869619827
20	0.019093033567446533
21	0.017357303243133212
22	0.018777446235753205
23	0.020513176560066525
24	0.017988477906519876
25	0.01783068424067321
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	633739.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.16792506537638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.8701096830639	82.76332796415039
2	4.669328736892666	8.233700523599222
3	0.7565277385635099	2.001044428906386
4	0.26645311785036574	0.939704741122678
5	0.11983154726799233	0.5282649439996229
6	0.07660586575649103	0.405250813895195
7	0.04960561623343471	0.30615369784239027
8	0.03379012457750192	0.23833641373591377
9	0.02307406525280944	0.18309532105469772
>10	0.1169424256926776	1.8840427799572865
>50	0.008238754936805434	0.5233174245980453
>100	0.008417721205418366	1.3738096184214483
>500	0.0010746027063549286	0.6199513287167093
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	804	0.12686610734071913	No Hit
GTCCTACAGTGTGCATTTCTCATTT	734	0.11582055073145253	No Hit
GTATCAACGCAGAGTACTTTTTTTT	724	0.11424261407298587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.5779366584666558E-4	0.0
10	0.0	0.0	0.0	3.1558733169333115E-4	0.0
11	0.0	0.0	0.0	3.1558733169333115E-4	0.0
12	0.0	0.0	0.0	3.1558733169333115E-4	1.5779366584666558E-4
13	0.0	0.0	0.0	3.1558733169333115E-4	1.5779366584666558E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCGTT	25	0.0060899034	18.96654	11
CCCGTTT	30	7.807838E-4	18.966537	12
TAGGACT	115	2.3646862E-11	14.073168	4
TAGGACC	190	0.0	14.029596	4
GTAGGAC	415	0.0	13.550682	3
GTCTAGA	50	0.0014531777	13.35351	1
ATAGCCC	50	0.0014610224	13.343977	3
ATCCCGT	50	0.0015178645	13.276577	10
GTCCTAC	445	0.0	13.074863	1
TCCAACG	60	4.147291E-4	12.644359	18
AGGACCT	290	0.0	12.470641	5
GTCCTAT	115	4.991307E-9	12.441157	1
GGACCTG	290	0.0	12.142467	6
TGTAGGA	480	0.0	12.113795	2
AGGACTG	160	3.6379788E-12	11.896336	5
GTATAGC	65	7.746414E-4	11.739348	1
CTGTAGG	480	0.0	11.724062	1
TCTATAC	115	6.727714E-8	11.6034565	3
GGCGAGA	230	0.0	11.545761	19
AGAACAG	215	0.0	11.509013	5
>>END_MODULE