FastQCFastQC Report
Thu 26 May 2016
SRR1926869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633739
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13420.2117590995662252No Hit
GGTATCAACGCAGAGTACTTTTTTT8660.1366493146232124No Hit
GTCCTACAGTGGACATTTCTAAATT8190.1292330123284191No Hit
CTGTAGGACGTGGAATATGGCAAGA7560.11929201138007918No Hit
GTCCTACAGTGTGCATTTCTCATTT7440.1173984873899192No Hit
TATCAACGCAGAGTACTTTTTTTTT6900.10887762943419925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG456.580099E-414.8288361
TACTAGA602.4851675E-514.29696752
TAGGACC2300.014.0752614
GTAAGAC500.001471649713.3311563
GTAGGAC4300.013.2868663
TCCTACA4350.013.1466372
TGTAGGA4650.012.913392
GACGTGG1550.012.8563197
GACTGTC905.4629527E-712.652257
TCCCGTT604.1282707E-412.65125211
TAGGACG3100.012.5929514
GTCCTAC4550.012.5707561
CTGTAGG4750.012.44284251
GTGTAAG1001.3739918E-712.3926711
AGGACGT3000.012.3779515
CTAGACA1001.3954377E-712.377954
GGACGTG2950.012.2649846
TCCAACG701.10138244E-412.19942218
CCTACAG4450.012.1970453
ATTAATC550.003011733512.1192323
Produced by FastQC (version 0.11.5)