##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926869_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 633739 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.92753168102326 31.0 30.0 33.0 26.0 34.0 2 30.234694408897038 31.0 30.0 34.0 26.0 34.0 3 30.298921164706606 31.0 30.0 34.0 26.0 34.0 4 33.89930870594993 35.0 35.0 37.0 30.0 37.0 5 33.86986125202962 37.0 35.0 37.0 30.0 37.0 6 33.84049900668887 37.0 35.0 37.0 30.0 37.0 7 33.830988782448294 37.0 35.0 37.0 29.0 37.0 8 33.85605588420469 37.0 35.0 37.0 30.0 37.0 9 35.4133152606988 39.0 35.0 39.0 30.0 39.0 10 35.295730576783185 39.0 35.0 39.0 30.0 39.0 11 35.33340381450408 39.0 35.0 39.0 30.0 39.0 12 35.22614199220815 39.0 35.0 39.0 29.0 39.0 13 35.23729800438351 39.0 35.0 39.0 30.0 39.0 14 36.339114367271065 40.0 36.0 41.0 29.0 41.0 15 36.32564194408108 40.0 36.0 41.0 29.0 41.0 16 36.3056573763016 40.0 36.0 41.0 29.0 41.0 17 36.281109415705835 40.0 36.0 41.0 29.0 41.0 18 36.25015818815001 40.0 36.0 41.0 29.0 41.0 19 36.32420286584856 40.0 37.0 41.0 29.0 41.0 20 36.2805902745452 40.0 37.0 41.0 29.0 41.0 21 36.235552806439244 40.0 37.0 41.0 29.0 41.0 22 36.14836076050235 40.0 37.0 41.0 29.0 41.0 23 36.08557939467194 40.0 37.0 41.0 29.0 41.0 24 36.00799382711179 40.0 36.0 41.0 29.0 41.0 25 35.88922726863898 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08624343958571146 1101 2 -0.005289581695585355 1101 3 0.24384304279615776 1101 4 -0.02110696814624191 1101 5 -0.04446955476048231 1101 6 0.1495573302173625 1101 7 0.06922179495396108 1101 8 0.30972745592411144 1101 9 0.11059974950552487 1101 10 0.1928974789596083 1101 11 0.0611845001713931 1101 12 0.1020145142238249 1101 13 0.1638008021354267 1101 14 0.057604025681115445 1101 15 0.3551763151146332 1101 16 0.21115505870794493 1101 17 0.21705146885346238 1101 18 0.06552388250999996 1101 19 -0.09961495786679819 1101 20 0.3355311058851953 1101 21 0.09338362968451008 1101 22 -0.050684649251813596 1101 23 0.010910175780040277 1101 24 0.025190940476790047 1101 25 -0.009240040470068323 1102 1 -0.4611031873706928 1102 2 -0.28538776393584087 1102 3 -0.1416110102368151 1102 4 -0.15944629088451023 1102 5 -0.16417496252174857 1102 6 -0.21821527115682215 1102 7 4.9288712311579275E-5 1102 8 -0.0882770366985639 1102 9 -0.07187606338834485 1102 10 -0.07804590352553475 1102 11 -0.22797289410745947 1102 12 -0.2818213753222949 1102 13 -0.08226473917553534 1102 14 -0.2738270362301378 1102 15 -0.26906381943646096 1102 16 -0.21322140213409568 1102 17 -0.1862240035660676 1102 18 -0.2691070920067702 1102 19 -0.32716379294839726 1102 20 -0.3039450279103022 1102 21 -0.2403768138424951 1102 22 -0.05300834112253483 1102 23 -0.0534135297460665 1102 24 -0.39046427918150783 1102 25 -0.2730518965331754 1103 1 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0.01052393889195713 2214 8 -0.003335433922522668 2214 9 0.1382566994503378 2214 10 -0.08063564895253705 2214 11 -0.09519163035263034 2214 12 -0.036629805813731764 2214 13 -0.0130049150325533 2214 14 0.0866972592669768 2214 15 0.024449533618515318 2214 16 -0.04649604821927511 2214 17 -0.10114728363731018 2214 18 0.02357201499168582 2214 19 0.07216676297679214 2214 20 -0.03906525146776829 2214 21 -0.2804226717852387 2214 22 -0.16813429692285098 2214 23 -0.278199338350575 2214 24 -0.39364055588505664 2214 25 -0.2426331357212419 2215 1 -0.05331181817788533 2215 2 0.0033803248701005373 2215 3 -0.021313492519958288 2215 4 0.04348131745602046 2215 5 -0.07833245785089105 2215 6 0.11849814790836177 2215 7 -0.19631989397425542 2215 8 -0.1378437555222618 2215 9 -0.35948023406125174 2215 10 -0.2240194552571424 2215 11 -0.4461032974708772 2215 12 -0.3110094057906352 2215 13 -0.24086528449648625 2215 14 -0.2774756665515241 2215 15 -0.2790492249151768 2215 16 -0.5232173334811421 2215 17 -0.31641887382461675 2215 18 -0.5272206134967448 2215 19 -0.35173157447995607 2215 20 -0.5228577866796655 2215 21 -0.4778100403652559 2215 22 -0.18878394580330848 2215 23 -0.16363354063629743 2215 24 -0.2460362083100236 2215 25 -0.29320589808371267 2216 1 0.11248204521368521 2216 2 0.4482966756373514 2216 3 -1.3368900147821243 2216 4 -1.1035788946045741 2216 5 -0.18726617492625053 2216 6 0.21670381591322752 2216 7 0.17214238693609474 2216 8 0.2919190401525995 2216 9 0.21510291859159736 2216 10 0.15460697678135205 2216 11 0.29153203803528527 2216 12 0.164398524100676 2216 13 0.07000509416317158 2216 14 0.022818784343023424 2216 15 -0.02259368630084424 2216 16 0.13188957107760046 2216 17 0.22870257842677688 2216 18 0.16739761448238255 2216 19 0.07488038680079967 2216 20 0.045705209025967974 2216 21 0.25219475058160157 2216 22 0.2044120353965937 2216 23 0.372539856669583 2216 24 -0.09626841186581458 2216 25 0.22577954023546454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14367.0 3 16.0 4 224.0 5 1540.0 6 2110.0 7 1564.0 8 1214.0 9 1058.0 10 929.0 11 826.0 12 829.0 13 787.0 14 762.0 15 758.0 16 786.0 17 805.0 18 938.0 19 1120.0 20 1428.0 21 1919.0 22 2406.0 23 2990.0 24 3595.0 25 4172.0 26 4925.0 27 5731.0 28 6253.0 29 7789.0 30 10231.0 31 13147.0 32 17065.0 33 22466.0 34 30427.0 35 42774.0 36 62695.0 37 107959.0 38 255134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29652494526292 18.980894939544346 10.474293314966527 28.248286800226214 2 14.462676759379779 20.295262406328586 39.95346442065889 25.288596413632746 3 18.803095079152403 23.957825364435628 29.17063178311089 28.068447773301074 4 12.070742056272376 16.93126034534722 37.53453708861219 33.46346050976822 5 13.022071923509667 38.324698872181045 35.261740158213414 13.391489046095872 6 32.347614071752 37.785377283809886 17.20259016497261 12.6644184794655 7 28.709953867739458 31.68984853530467 20.929021342680784 18.671176254275085 8 25.383629695558042 37.87834314895121 19.307257244935965 17.430769910554787 9 25.94144966599703 15.879067726933439 19.465279629842964 38.71420297722657 10 14.548765458398622 29.699850248612552 33.914191981594314 21.837192311394517 11 34.809661171005125 22.848707299214784 23.528511374252027 18.81312015552806 12 22.990524430558516 25.080988110172232 31.353641506307838 20.57484595296141 13 28.99204084981759 21.86036456821137 25.414999305704583 23.73259527626646 14 20.76185937744087 22.131981777987466 27.115105745425165 29.99105309914649 15 23.027568827389885 30.663081504064078 23.03072478038512 23.278624888160916 16 22.364710801833574 28.653301458811647 27.001349144358443 21.980638594996332 17 21.719385613613195 28.729695868008548 27.307972695340975 22.24294582303728 18 21.5498251637906 28.191391999192096 28.773495588068215 21.48528724894909 19 23.187536785764443 27.262886654874187 27.193930605282635 22.355645954078742 20 23.09549432411706 27.716991671584605 27.839598317275073 21.34791568702326 21 22.89056803870882 26.989492688438798 27.152673225451114 22.967266047401264 22 22.84694487793871 27.920800203238244 27.14761124058958 22.08464367823347 23 22.3800687350293 27.649754314874603 27.336217806096524 22.633959143999572 24 22.711242325310575 27.10942517345469 28.121829333526893 22.057503167707843 25 22.64199475148453 27.365185268177676 27.766792961371493 22.2260270189663 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 185.5 3 190.0 4 190.0 5 190.0 6 316.0 7 442.0 8 442.0 9 442.0 10 782.5 11 1123.0 12 1123.0 13 1123.0 14 1871.0 15 2619.0 16 2619.0 17 2619.0 18 4672.5 19 6726.0 20 6726.0 21 6726.0 22 10563.5 23 14401.0 24 14401.0 25 14401.0 26 20834.0 27 27267.0 28 27267.0 29 27267.0 30 35772.0 31 44277.0 32 44277.0 33 44277.0 34 51018.5 35 57760.0 36 57760.0 37 57760.0 38 63847.5 39 69935.0 40 69935.0 41 69935.0 42 73863.5 43 77792.0 44 77792.0 45 77792.0 46 81384.0 47 84976.0 48 84976.0 49 84976.0 50 82661.5 51 80347.0 52 80347.0 53 80347.0 54 71968.5 55 63590.0 56 63590.0 57 63590.0 58 54507.5 59 45425.0 60 45425.0 61 45425.0 62 37220.0 63 29015.0 64 29015.0 65 29015.0 66 22521.5 67 16028.0 68 16028.0 69 16028.0 70 11589.5 71 7151.0 72 7151.0 73 7151.0 74 5057.5 75 2964.0 76 2964.0 77 2964.0 78 2026.5 79 1089.0 80 1089.0 81 1089.0 82 713.0 83 337.0 84 337.0 85 337.0 86 213.0 87 89.0 88 89.0 89 89.0 90 51.0 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11250688374867257 2 0.10651072444649928 3 0.013885842594506571 4 0.0 5 0.005996159302173292 6 0.33436477792908437 7 0.019882001896679866 8 0.008678651621566606 9 0.009783207282493265 10 0.003471460648626643 11 0.004260428977859971 12 6.311746633866623E-4 13 4.733809975399968E-4 14 0.0 15 0.0025246986535466492 16 6.311746633866623E-4 17 1.5779366584666558E-4 18 4.733809975399968E-4 19 3.1558733169333115E-4 20 7.889683292333279E-4 21 0.013412461596966574 22 0.0 23 1.5779366584666558E-4 24 0.0 25 0.005680571970479961 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 633739.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.20314623246273 #Duplication Level Percentage of deduplicated Percentage of total 1 92.10943921699521 78.48014018995781 2 6.0222070375382 10.26221973723067 3 0.9543985104785755 2.4395326755705207 4 0.3403659962215716 1.160010149944977 5 0.17109671600520165 0.7288989256842672 6 0.09867316447705825 0.5044358437295184 7 0.059321384878619765 0.3538058041367671 8 0.043817194876741766 0.2986690290063477 9 0.029432879532477793 0.22569965449633486 >10 0.15028559593806295 2.3353439341357767 >50 0.00871893289275144 0.5084759417185409 >100 0.01075932529800832 1.6980022138871045 >500 0.0012985392591268918 0.7926539233938167 >1k 1.8550560844669885E-4 0.21211197710757768 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1342 0.2117590995662252 No Hit GGTATCAACGCAGAGTACTTTTTTT 866 0.1366493146232124 No Hit GTCCTACAGTGGACATTTCTAAATT 819 0.1292330123284191 No Hit CTGTAGGACGTGGAATATGGCAAGA 756 0.11929201138007918 No Hit GTCCTACAGTGTGCATTTCTCATTT 744 0.1173984873899192 No Hit TATCAACGCAGAGTACTTTTTTTTT 690 0.10887762943419925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.5779366584666558E-4 0.0 11 0.0 0.0 0.0 1.5779366584666558E-4 0.0 12 0.0 0.0 0.0 1.5779366584666558E-4 0.0 13 0.0 0.0 0.0 1.5779366584666558E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 45 6.580099E-4 14.828836 1 TACTAGA 60 2.4851675E-5 14.2969675 2 TAGGACC 230 0.0 14.075261 4 GTAAGAC 50 0.0014716497 13.331156 3 GTAGGAC 430 0.0 13.286866 3 TCCTACA 435 0.0 13.146637 2 TGTAGGA 465 0.0 12.91339 2 GACGTGG 155 0.0 12.856319 7 GACTGTC 90 5.4629527E-7 12.65225 7 TCCCGTT 60 4.1282707E-4 12.651252 11 TAGGACG 310 0.0 12.592951 4 GTCCTAC 455 0.0 12.570756 1 CTGTAGG 475 0.0 12.4428425 1 GTGTAAG 100 1.3739918E-7 12.392671 1 AGGACGT 300 0.0 12.377951 5 CTAGACA 100 1.3954377E-7 12.37795 4 GGACGTG 295 0.0 12.264984 6 TCCAACG 70 1.10138244E-4 12.199422 18 CCTACAG 445 0.0 12.197045 3 ATTAATC 55 0.0030117335 12.119232 3 >>END_MODULE