FastQCFastQC Report
Thu 26 May 2016
SRR1926868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593300
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8580.14461486600370807No Hit
GTATCAACGCAGAGTACTTTTTTTT7720.13011966964436203No Hit
GTCCTACAGTGTGCATTTCTCATTT7050.11882690038766223No Hit
CTGTAGGACGTGGAATATGGCAAGA6950.11714141243890105No Hit
GTCCTAAAGTGTGTATTTCTCATTT6820.11495027810551155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACT651.8604078E-716.1122254
GTATTAA508.415617E-515.2592021
GTGCAAC500.001517818813.276411
GTCTAGG603.9535368E-412.7160011
ATAAGAC603.9648652E-412.711693
CCCTTAT550.00297662512.13800051
CAGGACC1103.6814527E-812.1174584
CAGTAGT550.00301464512.1174584
GTATTGG802.7477803E-511.921251
AGGACGT4200.011.7827455
CTAGGAC657.7755365E-411.7338693
AGGACCA907.2934326E-611.6316855
GTCCTAC5050.011.3310911
GGACGTG4100.011.1416596
CTCCAAT600.005700957511.12651
GCATAGG600.005700957511.12651
ATAGTTC600.00571536911.1227293
TAAGCCA600.005773325511.107674
CTATTCA600.00594276211.0645989
TGGAGTG951.3297864E-511.0194915
Produced by FastQC (version 0.11.5)