##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926868_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 593300 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.479396595314345 31.0 28.0 31.0 12.0 34.0 2 27.42991909657846 31.0 28.0 31.0 15.0 34.0 3 27.09541547277937 31.0 27.0 31.0 10.0 34.0 4 30.807655486263272 35.0 32.0 37.0 12.0 37.0 5 31.076248103826057 35.0 32.0 37.0 12.0 37.0 6 30.50700825889095 35.0 31.0 37.0 10.0 37.0 7 30.26497893140064 35.0 30.0 37.0 10.0 37.0 8 30.20159110062363 35.0 30.0 37.0 10.0 37.0 9 32.16158604415978 37.0 32.0 39.0 10.0 39.0 10 32.365922804651944 37.0 32.0 39.0 10.0 39.0 11 32.53082588909489 37.0 32.0 39.0 10.0 39.0 12 32.407989212877126 37.0 32.0 39.0 10.0 39.0 13 32.49843586718355 37.0 33.0 39.0 10.0 39.0 14 33.433000168548794 38.0 33.0 40.0 8.0 41.0 15 33.02780886566661 38.0 32.0 40.0 2.0 41.0 16 33.08484914882859 38.0 32.0 40.0 2.0 41.0 17 33.30142929378055 38.0 33.0 40.0 2.0 41.0 18 33.33835496376201 38.0 33.0 40.0 2.0 41.0 19 32.7095921119164 38.0 32.0 40.0 2.0 41.0 20 32.99802123714815 39.0 32.0 40.0 2.0 41.0 21 32.96570200573066 39.0 32.0 40.0 2.0 41.0 22 32.97494353615372 39.0 33.0 40.0 2.0 41.0 23 32.951270857913364 39.0 33.0 40.0 2.0 41.0 24 31.649991572560257 38.0 31.0 40.0 2.0 41.0 25 32.193495702005734 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.09611284098645001 1101 2 -0.41798974845763937 1101 3 -0.9556251754730098 1101 4 -0.4759647720213813 1101 5 0.17997679178128223 1101 6 -2.093166947381185 1101 7 -0.6822227004841324 1101 8 -0.05713433408855906 1101 9 -0.24760566099835302 1101 10 -8.721050319205101 1101 11 -3.7126652478156963 1101 12 -1.275872096285358 1101 13 -0.6296094476407639 1101 14 -0.40377187513990265 1101 15 -1.7299386910092487 1101 16 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7 0.35718726039140947 2212 8 0.4291462575954377 2212 9 0.1600982150252861 2212 10 0.3883512998190213 2212 11 0.1957142006821826 2212 12 -0.04897608745004334 2212 13 -0.22202776211681652 2212 14 -0.22716665619567067 2212 15 -1.125155637891627 2212 16 -0.3962966413012907 2212 17 -0.1354366139621348 2212 18 -0.13263996843021886 2212 19 0.7777424297436681 2212 20 -0.6766842347786195 2212 21 -0.7183713187603118 2212 22 -0.03910937369465728 2212 23 0.017528036552725723 2212 24 1.309144844802919 2212 25 0.7822366924167667 2213 1 -0.23705934304406284 2213 2 -0.21493692238894013 2213 3 0.4456723805864513 2213 4 -7.275893385442057 2213 5 -1.4215391232266974 2213 6 -3.444220949228839 2213 7 -1.2883787431481117 2213 8 -2.6208153620553034 2213 9 -0.6559788868318961 2213 10 -0.15531167806963708 2213 11 -0.20050853327803253 2213 12 -0.06811615440243202 2213 13 -0.01839335626594618 2213 14 -0.4118763514983428 2213 15 -3.016660787255155 2213 16 -1.1686745171621808 2213 17 -0.5553325605708039 2213 18 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0.3334990134928262 2215 5 0.2659578508715832 2215 6 0.8817897019452126 2215 7 0.43730511064031674 2215 8 -1.047177017828723 2215 9 -0.5835266960790513 2215 10 -0.5496350864453312 2215 11 -0.6202616196977822 2215 12 -0.8660948520543315 2215 13 -0.43010964772079774 2215 14 -0.6429342067392056 2215 15 -0.7118314481120862 2215 16 -0.2976021375930671 2215 17 -0.6098956203626216 2215 18 -1.4223280716507816 2215 19 -0.1628295503437016 2215 20 -4.071538445893118 2215 21 -2.694466005339603 2215 22 -1.8488015407169058 2215 23 -1.7304197435956041 2215 24 -1.7152278058117325 2215 25 -1.5015182603415376 2216 1 -1.2559568955673477 2216 2 -1.8259938857850848 2216 3 -0.9528006538514937 2216 4 -1.2541801517405204 2216 5 -1.2380325172054718 2216 6 -2.718858159538563 2216 7 -10.28523817568006 2216 8 -11.67101029382918 2216 9 -6.89195462865559 2216 10 -4.464874164532706 2216 11 -3.5807879354872583 2216 12 -3.0554997368152534 2216 13 -2.911126732917449 2216 14 -3.0092339430079846 2216 15 -4.045250780757428 2216 16 -2.9113104279267468 2216 17 -3.2396513823165236 2216 18 -3.05841338296829 2216 19 -2.285103372153131 2216 20 -3.197928768103875 2216 21 -5.685545010040901 2216 22 -4.379142097992446 2216 23 -4.442729111787326 2216 24 -4.910870507336785 2216 25 -4.152819716596554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44297.0 3 3.0 4 309.0 5 1842.0 6 2450.0 7 1944.0 8 1528.0 9 1402.0 10 1334.0 11 1286.0 12 1337.0 13 1224.0 14 1235.0 15 1293.0 16 1404.0 17 1465.0 18 1698.0 19 2175.0 20 2707.0 21 3696.0 22 4266.0 23 5192.0 24 5976.0 25 6923.0 26 7662.0 27 8574.0 28 9289.0 29 11221.0 30 13161.0 31 15644.0 32 20224.0 33 26270.0 34 34944.0 35 47711.0 36 68453.0 37 105398.0 38 127763.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26232269159106 18.76931029414994 12.392542277824983 25.575824736434015 2 14.544580021264746 20.000506303478303 41.91905895060166 23.53585472465529 3 18.888320683736453 24.618191453631926 29.160063391442154 27.333424471189467 4 11.589507292037368 17.281293693614682 38.85128696406014 32.277912050287824 5 12.499536386104396 37.95885131649488 36.836399928519164 12.705212368881561 6 31.940084630038605 37.43597956740901 18.368595849419222 12.255339953133165 7 28.199900252749387 31.186400561289613 22.605093872306615 18.008605313654385 8 24.387976239176957 38.1949820982416 20.81523153955122 16.601810123030212 9 26.168725313517278 15.578002963642279 20.18951801196583 38.06375371087461 10 14.148850070816755 29.795137249612193 35.07705537195657 20.978957307614486 11 34.19745718996227 22.88057019362713 24.374327800648008 18.54764481576259 12 22.748429273917434 25.212197634175492 32.154410760674786 19.884962331232288 13 28.780124340872003 22.28678642230044 25.67750266348397 23.255586573343585 14 20.47326923952238 22.516280536014417 27.81775914415713 29.192691080306073 15 22.98550436352541 31.06422145165396 23.329731479249443 22.620542705571186 16 21.691934475090992 28.641101589907937 28.13619497655851 21.53076895844256 17 21.422815623999057 28.861747500800757 28.055091960417407 21.660344914782783 18 21.29158090460224 28.2381816925175 29.56283683208841 20.907400570791843 19 23.607746347088547 27.25838184494156 27.304912527374707 21.82895928059519 20 23.084988915955122 27.871610515934897 28.152125355068737 20.891275213041244 21 22.722192534553358 27.211979508203633 27.151293043139646 22.914534914103367 22 22.416760648618762 28.449474796821978 27.532076082393935 21.60168847216533 23 21.955174942472546 28.067836040425153 27.68280245113327 22.29418656596903 24 22.97271783253092 27.500472009817805 28.058960769241143 21.467849388410137 25 22.5875784501636 27.641598014871605 27.835963931889328 21.934859603075466 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 193.0 2 235.5 3 278.0 4 278.0 5 278.0 6 463.0 7 648.0 8 648.0 9 648.0 10 944.0 11 1240.0 12 1240.0 13 1240.0 14 2110.0 15 2980.0 16 2980.0 17 2980.0 18 5241.5 19 7503.0 20 7503.0 21 7503.0 22 11387.5 23 15272.0 24 15272.0 25 15272.0 26 21856.0 27 28440.0 28 28440.0 29 28440.0 30 36914.5 31 45389.0 32 45389.0 33 45389.0 34 51898.5 35 58408.0 36 58408.0 37 58408.0 38 63316.5 39 68225.0 40 68225.0 41 68225.0 42 71524.0 43 74823.0 44 74823.0 45 74823.0 46 76641.0 47 78459.0 48 78459.0 49 78459.0 50 74964.5 51 71470.0 52 71470.0 53 71470.0 54 63283.5 55 55097.0 56 55097.0 57 55097.0 58 46921.0 59 38745.0 60 38745.0 61 38745.0 62 31201.0 63 23657.0 64 23657.0 65 23657.0 66 18311.0 67 12965.0 68 12965.0 69 12965.0 70 9428.0 71 5891.0 72 5891.0 73 5891.0 74 4101.5 75 2312.0 76 2312.0 77 2312.0 78 1606.0 79 900.0 80 900.0 81 900.0 82 580.5 83 261.0 84 261.0 85 261.0 86 182.0 87 103.0 88 103.0 89 103.0 90 68.0 91 33.0 92 33.0 93 33.0 94 19.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2229900556211023 2 0.1297825720546098 3 0.13332209674700826 4 0.06623967638631384 5 0.02258553851339963 6 0.021911343333895165 7 0.30423057475139054 8 0.29293780549469073 9 0.018708916231248946 10 0.03708073487274566 11 0.01651778189785943 12 0.01736052587224001 13 0.015506489128602731 14 0.01803472105174448 15 0.014663745154222148 16 0.020394404180010112 17 0.01854036743637283 18 0.01500084274397438 19 0.02410247766728468 20 0.017697623461992246 21 0.014663745154222148 22 0.01702342828248778 23 0.017697623461992246 24 0.015506489128602731 25 0.01500084274397438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 593300.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.09572788751741 #Duplication Level Percentage of deduplicated Percentage of total 1 94.18237550658091 82.97064924427737 2 4.538146236652814 7.995825919558551 3 0.6697467667730309 1.7700548675754455 4 0.21262103778240715 0.7492402035056199 5 0.11020528961798316 0.4854307602975446 6 0.059445021540580435 0.31421114651439513 7 0.04132899137514884 0.2548635304635466 8 0.027680759760805397 0.19508453436861234 9 0.021163642368154656 0.16779838312563183 >10 0.11470847712119801 1.9079690252719474 >50 0.011480537578490367 0.7008889863980665 >100 0.009758351297920909 1.6825914827767088 >500 0.001339381550533385 0.8053919158665536 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 858 0.14461486600370807 No Hit GTATCAACGCAGAGTACTTTTTTTT 772 0.13011966964436203 No Hit GTCCTACAGTGTGCATTTCTCATTT 705 0.11882690038766223 No Hit CTGTAGGACGTGGAATATGGCAAGA 695 0.11714141243890105 No Hit GTCCTAAAGTGTGTATTTCTCATTT 682 0.11495027810551155 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.6854879487611664E-4 0.0 11 0.0 0.0 0.0 1.6854879487611664E-4 0.0 12 0.0 0.0 0.0 1.6854879487611664E-4 1.6854879487611664E-4 13 0.0 0.0 0.0 1.6854879487611664E-4 1.6854879487611664E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGACT 65 1.8604078E-7 16.112225 4 GTATTAA 50 8.415617E-5 15.259202 1 GTGCAAC 50 0.0015178188 13.2764 11 GTCTAGG 60 3.9535368E-4 12.716001 1 ATAAGAC 60 3.9648652E-4 12.71169 3 CCCTTAT 55 0.002976625 12.1380005 1 CAGGACC 110 3.6814527E-8 12.117458 4 CAGTAGT 55 0.003014645 12.117458 4 GTATTGG 80 2.7477803E-5 11.92125 1 AGGACGT 420 0.0 11.782745 5 CTAGGAC 65 7.7755365E-4 11.733869 3 AGGACCA 90 7.2934326E-6 11.631685 5 GTCCTAC 505 0.0 11.331091 1 GGACGTG 410 0.0 11.141659 6 CTCCAAT 60 0.0057009575 11.1265 1 GCATAGG 60 0.0057009575 11.1265 1 ATAGTTC 60 0.005715369 11.122729 3 TAAGCCA 60 0.0057733255 11.10767 4 CTATTCA 60 0.005942762 11.064598 9 TGGAGTG 95 1.3297864E-5 11.019491 5 >>END_MODULE