FastQCFastQC Report
Thu 26 May 2016
SRR1926868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593300
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13000.21911343333895164No Hit
CTGTAGGACGTGGAATATGGCAAGA8740.14731164672172595No Hit
GTCCTACAGTGGACATTTCTAAATT8710.1468060003370976No Hit
GGTATCAACGCAGAGTACTTTTTTT8330.14040114613180515No Hit
GTCCTACAGTGTGCATTTCTCATTT7230.12186077869543234No Hit
GTCCTAAAGTGTGTATTTCTCATTT7170.12084948592617561No Hit
CTTTAGGACGTGAAATATGGCGAGG7150.1205123883364234No Hit
TATCAACGCAGAGTACTTTTTTTTT6580.11090510702848475No Hit
GTACATGGGAAGCAGTGGTATCAAC6500.10955671666947582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2250.014.3842394
AGGTGTC500.001509459813.2861337
TAAGGCT655.3403957E-513.1801294
GGCGAGG2450.012.78150119
TGTAGGA5900.012.4378022
TAAGACT853.844889E-612.3186834
GACGTGG1850.012.3114757
CTGTAGG5750.012.2650361
GTAGGAC5600.012.2386913
TAACCCT550.00301910812.11506754
CTAAACT550.00301910812.11506754
TAAGACC550.00301910812.11506754
AGGACGT4800.011.7004165
TAAGGGA752.0316588E-411.4227774
AATGCCG855.371098E-511.16387718
ATGCCGG855.371098E-511.16387719
GTATAAG600.00573104611.1186411
GGACGTG5000.011.0420196
ACTGTTC2150.011.0349948
TTTAGAA1657.4578566E-1110.9742422
Produced by FastQC (version 0.11.5)