##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 593300 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.830497218944885 31.0 30.0 33.0 26.0 34.0 2 30.15499410079218 31.0 30.0 34.0 26.0 34.0 3 30.201488285858755 31.0 30.0 34.0 26.0 34.0 4 33.7704045171077 35.0 35.0 37.0 28.0 37.0 5 33.74347884712624 37.0 35.0 37.0 28.0 37.0 6 33.67321422551829 37.0 35.0 37.0 29.0 37.0 7 33.65604078880836 37.0 35.0 37.0 28.0 37.0 8 33.676716669475816 37.0 35.0 37.0 29.0 37.0 9 35.21995786280128 39.0 35.0 39.0 29.0 39.0 10 35.11941850665768 39.0 35.0 39.0 29.0 39.0 11 35.13319905612675 39.0 35.0 39.0 29.0 39.0 12 35.027625147480194 39.0 35.0 39.0 28.0 39.0 13 35.04795887409405 39.0 35.0 39.0 29.0 39.0 14 36.137540873082756 40.0 36.0 41.0 28.0 41.0 15 36.117006573403 40.0 36.0 41.0 28.0 41.0 16 36.079187594808694 40.0 36.0 41.0 28.0 41.0 17 36.061208494859265 40.0 36.0 41.0 28.0 41.0 18 36.03098601045003 40.0 36.0 41.0 28.0 41.0 19 36.105112084948594 40.0 36.0 41.0 28.0 41.0 20 36.06338951626496 40.0 36.0 41.0 27.0 41.0 21 36.00949098263948 40.0 36.0 41.0 27.0 41.0 22 35.91803977751559 40.0 36.0 41.0 27.0 41.0 23 35.8529310635429 40.0 36.0 41.0 27.0 41.0 24 35.7832664756447 40.0 36.0 41.0 27.0 41.0 25 35.666836339120174 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1848047490899276 1101 2 -0.12704950873365561 1101 3 0.07349934696615534 1101 4 0.040831973271870936 1101 5 -0.00660544868265589 1101 6 -0.018488688008993392 1101 7 0.16252237960089388 1101 8 0.03390176165081016 1101 9 0.03494429271842847 1101 10 0.21150084269899594 1101 11 0.10878617526189771 1101 12 0.013186019254241899 1101 13 0.29779284348193613 1101 14 0.32402679221547714 1101 15 0.166588978437737 1101 16 0.24329292531626834 1101 17 0.24444381757483313 1101 18 0.08050109379044557 1101 19 0.013728632779525185 1101 20 0.22369441040508775 1101 21 0.26703380763892426 1101 22 0.17743227155651908 1101 23 -0.08746752708805872 1101 24 0.31047110892689034 1101 25 0.16256783191813895 1102 1 -0.8037645577666268 1102 2 -0.6327029470262104 1102 3 -0.44797287138207764 1102 4 -0.652487723627388 1102 5 -0.6524713880728328 1102 6 -0.8151870180623533 1102 7 -0.5934190250551197 1102 8 -0.6200556451539825 1102 9 -0.5094619283880846 1102 10 -0.5159822538046015 1102 11 -0.4459790869553615 1102 12 -0.4833093138828133 1102 13 -0.406844060634306 1102 14 -0.6293949713266898 1102 15 -0.49386639581523184 1102 16 -0.47391377513156385 1102 17 -0.4545654748285344 1102 18 -0.413287613795859 1102 19 -0.5838235239981486 1102 20 -0.33884206963871577 1102 21 -0.3228065324454761 1102 22 -0.44956735137542125 1102 23 -0.49626612625437616 1102 24 -0.3803098351895571 1102 25 -0.42006172711884204 1103 1 -0.3110992531458443 1103 2 -0.3056501586156024 1103 3 -0.09078286076229602 1103 4 -0.19817496971150916 1103 5 -0.0597648567757858 1103 6 -0.007466409681853747 1103 7 -0.06727400630742153 1103 8 0.24393285312519453 1103 9 0.14980742009996817 1103 10 0.29152047941966686 1103 11 0.225694794379649 1103 12 0.09013157410848294 1103 13 0.10992319390388872 1103 14 0.09294466864439954 1103 15 0.04805565445522575 1103 16 -0.036308262237788824 1103 17 0.09880480595644059 1103 18 0.13550728870827555 1103 19 0.28266988625686196 1103 20 0.3570955371788216 1103 21 0.3867593610905473 1103 22 0.1340327811761739 1103 23 0.07395099220968859 1103 24 0.10326864031057426 1103 25 0.009950771348201215 1104 1 -0.637428936019603 1104 2 -0.8050932752598747 1104 3 -0.45787006354314386 1104 4 -0.5667286980554849 1104 5 -0.6963558586505627 1104 6 -0.7931475711701026 1104 7 -0.9097810515754929 1104 8 -0.9227357953327697 1104 9 -0.7161600705955209 1104 10 -0.5511728542307566 1104 11 -0.6992468418665183 1104 12 -0.8584528323648399 1104 13 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-0.6066767080030928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14993.0 3 19.0 4 251.0 5 1684.0 6 2184.0 7 1519.0 8 1281.0 9 1072.0 10 972.0 11 921.0 12 845.0 13 810.0 14 756.0 15 780.0 16 749.0 17 862.0 18 900.0 19 1134.0 20 1479.0 21 1821.0 22 2336.0 23 2844.0 24 3442.0 25 3989.0 26 4721.0 27 5434.0 28 6041.0 29 7672.0 30 9590.0 31 12233.0 32 15839.0 33 21008.0 34 28578.0 35 40051.0 36 58280.0 37 100316.0 38 235894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92396023698514 19.334943158328144 10.259175074374252 28.481921530312466 2 14.053388228941685 21.01916846652268 40.189828563714904 24.737614740820735 3 18.402593396145306 25.03712919273296 29.25578094366309 27.304496467458645 4 11.944715995280633 17.622956345862125 38.23023765380078 32.20209000505647 5 12.811683025126797 38.78261367907752 35.42748592127375 12.97821737452193 6 31.290061798759965 38.3531154760737 17.777883011936936 12.578939713229401 7 27.728363925715133 32.089632756551175 21.708396833317597 18.47360648441609 8 24.750983171873443 38.395200536719976 19.733730254385716 17.12008603702087 9 26.086121014437786 15.86538218412602 19.729449543604137 38.319047257832054 10 14.292432555434756 30.078126975297714 34.31788492494922 21.311555544318306 11 34.10994093691847 23.157818441705547 24.153159470320126 18.579081151055853 12 22.812298792841325 25.442192235215593 31.743958307348464 20.00155066459462 13 28.788233767240694 22.327997559376428 25.591825933648078 23.2919427397348 14 20.52287295276075 22.71013435047084 27.506198392378884 29.260794304389528 15 22.813656168620476 31.121804866126734 23.288301575128312 22.776237390124475 16 21.73671941762137 28.75014116175705 27.83692993825964 21.676209482361944 17 21.201318730216517 29.11723956595168 27.78131731440187 21.90012438942993 18 21.087255516676443 28.369841494576026 29.48446970463111 21.058433284116422 19 23.0374178324625 27.49688184729479 27.41631552334401 22.049384796898703 20 22.942950231674615 27.956021433062133 28.15440704277342 20.94662129248983 21 22.548720481566203 27.21637945420924 27.272175594330033 22.96272446989453 22 22.507668970166865 28.46570032024271 27.291589415135682 21.735041294454742 23 21.95422873496961 28.12836045292585 27.52883036855004 22.388580443554503 24 22.428825937680664 27.38804548802543 28.479569323393434 21.703559250900472 25 22.34169331308374 27.627544613434512 28.048437202387472 21.98232487109427 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 195.0 2 220.0 3 245.0 4 245.0 5 245.0 6 388.0 7 531.0 8 531.0 9 531.0 10 836.5 11 1142.0 12 1142.0 13 1142.0 14 1914.0 15 2686.0 16 2686.0 17 2686.0 18 4872.0 19 7058.0 20 7058.0 21 7058.0 22 11012.0 23 14966.0 24 14966.0 25 14966.0 26 21358.5 27 27751.0 28 27751.0 29 27751.0 30 36429.0 31 45107.0 32 45107.0 33 45107.0 34 51506.5 35 57906.0 36 57906.0 37 57906.0 38 62785.5 39 67665.0 40 67665.0 41 67665.0 42 71077.5 43 74490.0 44 74490.0 45 74490.0 46 76883.5 47 79277.0 48 79277.0 49 79277.0 50 76058.0 51 72839.0 52 72839.0 53 72839.0 54 64042.0 55 55245.0 56 55245.0 57 55245.0 58 47370.0 59 39495.0 60 39495.0 61 39495.0 62 31817.5 63 24140.0 64 24140.0 65 24140.0 66 18652.0 67 13164.0 68 13164.0 69 13164.0 70 9523.0 71 5882.0 72 5882.0 73 5882.0 74 4094.5 75 2307.0 76 2307.0 77 2307.0 78 1580.5 79 854.0 80 854.0 81 854.0 82 564.0 83 274.0 84 274.0 85 274.0 86 166.5 87 59.0 88 59.0 89 59.0 90 39.0 91 19.0 92 19.0 93 19.0 94 10.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11613011966964436 2 0.11124220461823699 3 0.01702342828248778 4 0.0 5 0.004887915051407382 6 0.3414798584190123 7 0.020900050564638464 8 0.011292769256699815 9 0.010955671666947582 10 0.005899207820664082 11 0.006067756615540198 12 0.0010112927692566998 13 0.0015169391538850496 14 1.6854879487611664E-4 15 0.0025282319231417494 16 0.0015169391538850496 17 3.370975897522333E-4 18 0.0013483903590089331 19 0.0 20 0.0018540367436372831 21 0.011629866846452048 22 0.0 23 3.370975897522333E-4 24 1.6854879487611664E-4 25 0.006236305410416315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 593300.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.08867683226742 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2259811311969 78.47386704011208 2 6.0808341470594165 10.348202632195106 3 0.9269042396899914 2.366071659163207 4 0.27631875399322114 0.9404638864489602 5 0.1300900972023335 0.5534597119963807 6 0.08639264933452653 0.44106217319453594 7 0.04741858475785532 0.28243492440132434 8 0.03414172306242282 0.23240592321242143 9 0.028160043810507056 0.2156490780637255 >10 0.1382125141175417 2.2339259432770167 >50 0.012476047725818885 0.7346732482903762 >100 0.010891723374667872 1.7473682483292137 >500 0.0019803133407718817 1.2113626168722038 >1k 1.980313340771882E-4 0.21905291444343652 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1300 0.21911343333895164 No Hit CTGTAGGACGTGGAATATGGCAAGA 874 0.14731164672172595 No Hit GTCCTACAGTGGACATTTCTAAATT 871 0.1468060003370976 No Hit GGTATCAACGCAGAGTACTTTTTTT 833 0.14040114613180515 No Hit GTCCTACAGTGTGCATTTCTCATTT 723 0.12186077869543234 No Hit GTCCTAAAGTGTGTATTTCTCATTT 717 0.12084948592617561 No Hit CTTTAGGACGTGAAATATGGCGAGG 715 0.1205123883364234 No Hit TATCAACGCAGAGTACTTTTTTTTT 658 0.11090510702848475 No Hit GTACATGGGAAGCAGTGGTATCAAC 650 0.10955671666947582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 5.056463846283499E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 225 0.0 14.384239 4 AGGTGTC 50 0.0015094598 13.286133 7 TAAGGCT 65 5.3403957E-5 13.180129 4 GGCGAGG 245 0.0 12.781501 19 TGTAGGA 590 0.0 12.437802 2 TAAGACT 85 3.844889E-6 12.318683 4 GACGTGG 185 0.0 12.311475 7 CTGTAGG 575 0.0 12.265036 1 GTAGGAC 560 0.0 12.238691 3 TAACCCT 55 0.003019108 12.1150675 4 CTAAACT 55 0.003019108 12.1150675 4 TAAGACC 55 0.003019108 12.1150675 4 AGGACGT 480 0.0 11.700416 5 TAAGGGA 75 2.0316588E-4 11.422777 4 AATGCCG 85 5.371098E-5 11.163877 18 ATGCCGG 85 5.371098E-5 11.163877 19 GTATAAG 60 0.005731046 11.118641 1 GGACGTG 500 0.0 11.042019 6 ACTGTTC 215 0.0 11.034994 8 TTTAGAA 165 7.4578566E-11 10.974242 2 >>END_MODULE